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Mouse Anti-ADORA3 Recombinant Antibody (V2-180014) (CBMAB-A1401-YC)

Provided herein is a Mouse monoclonal antibody against Human Adenosine A3 Receptor. The antibody can be used for immunoassay techniques, such as ELISA.
See all ADORA3 antibodies
Published Data
Fig. 1 Flow cytometric analysis of HT-29 cells revealed expression of hA3AR protein.
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Fig. 2 Immunofluorescent staining of HT-29 cells with anti-human A3AR.
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Fig. 3 Two-color immunofluorescence detecting ADORA3 protein expression in combination with cell specific marker CD3 in rheumatoid synovia.
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Summary

Host Animal
Mouse
Specificity
Human
Clone
V2-180014
Antibody Isotype
IgG2b, κ
Application
ELISA

Basic Information

Immunogen
ADORA3 (AAH29831, 121 a.a. ~ 225 a.a) partial recombinant protein with GST tag.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
PBS, pH 7.2
Buffer
PBS, pH7.4
Preservative
None
Concentration
Batch dependent
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
adenosine A3 receptor
Introduction
ADORA3 is a protein that belongs to the family of adenosine receptors, which are G-protein-coupled receptors that are involved in a variety of intracellular signaling pathways and physiological functions. The receptor encoded by this gene mediates a susta
Entrez Gene ID
140
UniProt ID
P0DMS8
Alternative Names
Adenosine A3 Receptor; Adenosine Receptor A3; A3AR;
Function
Isoform 2: Receptor for adenosine. The activity of this receptor is mediated by G proteins which inhibits adenylyl cyclase.
Biological Process
Activation of adenylate cyclase activity
G protein-coupled receptor signaling pathway
Inflammatory response
Negative regulation of cell migration
Negative regulation of cell population proliferation
Negative regulation of NF-kappaB transcription factor activity
Regulation of heart contraction
Response to wounding
Signal transduction
Cellular Location
Cell membrane
Topology
Extracellular: 1-14 aa
Helical: 15-37 aa
Cytoplasmic: 38-48 aa
Helical: 49-72 aa
Extracellular: 73-84 aa
Helical: 85-106 aa
Cytoplasmic: 107-126 aa
Helical: 127-148 aa
Extracellular: 149-177 aa
Helical: 178-198 aa
Cytoplasmic: 199-231 aa
Helical: 232-255 aa
Extracellular: 256-261 aa
Helical: 262-284 aa
Cytoplasmic: 285-318 aa

Bi, J., Zheng, C., & Zheng, X. (2021). Increased expression of adenosine A3 receptor in tumor-infiltrating natural killer cells. Cellular & Molecular Immunology, 18(2), 496-497.

Durante, M., Squillace, S., Lauro, F., Giancotti, L. A., Coppi, E., Cherchi, F., ... & Salvemini, D. (2021). Adenosine A 3 agonists reverse neuropathic pain via T cell–mediated production of IL-10. The Journal of Clinical Investigation, 131(7).

Zhou, Z., Gao, Z., Yan, W., Zhang, Y., Huang, J., & Xiong, K. (2021). Adenosine A3 receptor activated in H2O2 oxidative stress of primary open-angle glaucoma. Annals of Translational Medicine, 9(7).

Ren, T. H., Lv, M. M., An, X. M., Leung, W. K., & Seto, W. K. (2020). Activation of adenosine A3 receptor inhibits inflammatory cytokine production in colonic mucosa of patients with ulcerative colitis by down‐regulating the nuclear factor‐kappa B signaling. Journal of digestive diseases, 21(1), 38-45.

Mailavaram, R. P., Al-Attraqchi, O. H., Kar, S., & Ghosh, S. (2019). Current status in the design and development of agonists and antagonists of adenosine A3 receptor as potential therapeutic agents. Current pharmaceutical design, 25(25), 2772-2787.

Garrido, W., Jara, C., Torres, A., Suarez, R., Cappelli, C., Oyarzún, C., ... & San Martín, R. (2019). Blockade of the adenosine A3 receptor attenuates caspase 1 activation in renal tubule epithelial cells and decreases interleukins IL-1β and IL-18 in diabetic rats. International journal of molecular sciences, 20(18), 4531.

Jacobson, K. A., Merighi, S., Varani, K., Borea, P. A., Baraldi, S., Aghazadeh Tabrizi, M., ... & Gessi, S. (2018). A3 adenosine receptors as modulators of inflammation: from medicinal chemistry to therapy. Medicinal research reviews, 38(4), 1031-1072.

Wu, W., He, Y., Feng, X., Ye, S., Wang, H., Tan, W., ... & Zhou, Y. (2017). MicroRNA-206 is involved in the pathogenesis of ulcerative colitis via regulation of adenosine A3 receptor. Oncotarget, 8(1), 705.

Arruda, M. A., Stoddart, L. A., Gherbi, K., Briddon, S. J., Kellam, B., & Hill, S. J. (2017). A non-imaging high throughput approach to chemical library screening at the unmodified adenosine-A3 receptor in living cells. Frontiers in pharmacology, 8, 908.

Salvemini, D., & Jacobson, K. A. (2017). Highly selective A3 adenosine receptor agonists relieve chronic neuropathic pain. Expert opinion on therapeutic patents, 27(8), 967-967.

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For research use only. Not intended for any clinical use.

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