Has this antibody been validated in applications other than ELISA?

This antibody is currently validated for use in ELISA. While it may be suitable for other applications, such as Western Blotting or Immunohistochemistry, these have not been validated by Creative Biolabs. If you are interested in using this antibody in a different application, we recommend performing a thorough validation in your own laboratory.

How should I store this antibody to ensure its stability?

For long-term storage, we recommend aliquoting the antibody and storing it at -20°C or -80°C. Avoid repeated freeze-thaw cycles, as this can degrade the antibody and reduce its activity. For short-term use, the antibody can be stored at 4°C for a few weeks. Please refer to the product datasheet for detailed storage instructions.

Mouse Anti-ADORA3 Recombinant Antibody (V2-180014) (CBMAB-A1401-YC)

See all ADORA3 antibodies

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

V2-180014

Application

ELISA

Immunogen

ADORA3 (AAH29831, 121 a.a. ~ 225 a.a) partial recombinant protein with GST tag.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

PBS, pH 7.2

Buffer

PBS, pH7.4

Preservative

None

Concentration

Batch dependent

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

adenosine A3 receptor

Introduction

ADORA3 is a protein that belongs to the family of adenosine receptors, which are G-protein-coupled receptors that are involved in a variety of intracellular signaling pathways and physiological functions. The receptor encoded by this gene mediates a susta

Entrez Gene ID

140

UniProt ID

P0DMS8

Alternative Names

Adenosine A3 Receptor; Adenosine Receptor A3; A3AR;

Function

Isoform 2: Receptor for adenosine. The activity of this receptor is mediated by G proteins which inhibits adenylyl cyclase.

Biological Process

Activation of adenylate cyclase activity
G protein-coupled receptor signaling pathway
Inflammatory response
Negative regulation of cell migration
Negative regulation of cell population proliferation
Negative regulation of NF-kappaB transcription factor activity
Regulation of heart contraction
Response to wounding
Signal transduction

Cellular Location

Cell membrane

Topology

Extracellular: 1-14 aa
Helical: 15-37 aa
Cytoplasmic: 38-48 aa
Helical: 49-72 aa
Extracellular: 73-84 aa
Helical: 85-106 aa
Cytoplasmic: 107-126 aa
Helical: 127-148 aa
Extracellular: 149-177 aa
Helical: 178-198 aa
Cytoplasmic: 199-231 aa
Helical: 232-255 aa
Extracellular: 256-261 aa
Helical: 262-284 aa
Cytoplasmic: 285-318 aa

Bi, J., Zheng, C., & Zheng, X. (2021). Increased expression of adenosine A3 receptor in tumor-infiltrating natural killer cells. Cellular & Molecular Immunology, 18(2), 496-497.

Durante, M., Squillace, S., Lauro, F., Giancotti, L. A., Coppi, E., Cherchi, F., ... & Salvemini, D. (2021). Adenosine A 3 agonists reverse neuropathic pain via T cell–mediated production of IL-10. The Journal of Clinical Investigation, 131(7).

Zhou, Z., Gao, Z., Yan, W., Zhang, Y., Huang, J., & Xiong, K. (2021). Adenosine A3 receptor activated in H2O2 oxidative stress of primary open-angle glaucoma. Annals of Translational Medicine, 9(7).

Ren, T. H., Lv, M. M., An, X. M., Leung, W. K., & Seto, W. K. (2020). Activation of adenosine A3 receptor inhibits inflammatory cytokine production in colonic mucosa of patients with ulcerative colitis by down‐regulating the nuclear factor‐kappa B signaling. Journal of digestive diseases, 21(1), 38-45.

Mailavaram, R. P., Al-Attraqchi, O. H., Kar, S., & Ghosh, S. (2019). Current status in the design and development of agonists and antagonists of adenosine A3 receptor as potential therapeutic agents. Current pharmaceutical design, 25(25), 2772-2787.

Garrido, W., Jara, C., Torres, A., Suarez, R., Cappelli, C., Oyarzún, C., ... & San Martín, R. (2019). Blockade of the adenosine A3 receptor attenuates caspase 1 activation in renal tubule epithelial cells and decreases interleukins IL-1β and IL-18 in diabetic rats. International journal of molecular sciences, 20(18), 4531.

Jacobson, K. A., Merighi, S., Varani, K., Borea, P. A., Baraldi, S., Aghazadeh Tabrizi, M., ... & Gessi, S. (2018). A3 adenosine receptors as modulators of inflammation: from medicinal chemistry to therapy. Medicinal research reviews, 38(4), 1031-1072.

Wu, W., He, Y., Feng, X., Ye, S., Wang, H., Tan, W., ... & Zhou, Y. (2017). MicroRNA-206 is involved in the pathogenesis of ulcerative colitis via regulation of adenosine A3 receptor. Oncotarget, 8(1), 705.

Arruda, M. A., Stoddart, L. A., Gherbi, K., Briddon, S. J., Kellam, B., & Hill, S. J. (2017). A non-imaging high throughput approach to chemical library screening at the unmodified adenosine-A3 receptor in living cells. Frontiers in pharmacology, 8, 908.

Salvemini, D., & Jacobson, K. A. (2017). Highly selective A3 adenosine receptor agonists relieve chronic neuropathic pain. Expert opinion on therapeutic patents, 27(8), 967-967.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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