Mouse Anti-AFF2 Recombinant Antibody (V2-611023) (CBMAB-A0176-LY)

The product is antibody recognizes AFF2. The antibody 4D11 immunoassay techniques such as: WB, ELISA.
See all AFF2 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

V2-611023

Antibody Isotype

IgG2a, κ

Application

WB, ELISA

Basic Information

Immunogen

AFF2 (NP_002016.1, 109 a.a. ~ 219 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH7.4

Preservative

None

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

AF4/FMR2 family, member 2

Entrez Gene ID

2334

Alternative Names

FMR2; FRAXE; MRX2; OX19

Function

RNA-binding protein. Might be involved in alternative splicing regulation through an interaction with G-quartet RNA structure.

Biological Process

Brain development
Learning or memory
mRNA processing
Negative regulation of gene expression
Nuclear speck organization
Regulation of gene expression
Regulation of RNA splicing
RNA splicing

Cellular Location

Nucleus speckle. When splicing is inhibited, accumulates in enlarged speckles.

Involvement in disease

Mental retardation, X-linked, associated with fragile site FRAXE (MRFRAXE): A form of mild to moderate mental retardation associated with learning difficulties, communication deficits, attention problems, hyperactivity, and autistic behavior. It is associated with a fragile site on chromosome Xq28. Mental retardation is characterized by significantly below average general intellectual functioning associated with impairments in adaptive behavior and manifested during the developmental period.

References

Silva, C., Maia, N., Santos, F., Rodrigues, B., Marques, I., Santos, R., & Jorge, P. (2021). Development and validation in 500 female samples of a TP-PCR assay to identify AFF2 GCC expansions. Scientific reports, 11(1), 1-6.

Kuo, Y. J., Lewis, J. S., Zhai, C., Chen, Y. A., Chernock, R. D., Hsieh, M. S., ... & Hang, J. F. (2021). DEK-AFF2 fusion-associated papillary squamous cell carcinoma of the sinonasal tract: clinicopathologic characterization of seven cases with deceptively bland morphology. Modern Pathology, 1-11.

Yuva-Aydemir, Y., Almeida, S., Krishnan, G., Gendron, T. F., & Gao, F. B. (2019). Transcription elongation factor AFF2/FMR2 regulates expression of expanded GGGGCC repeat-containing C9ORF72 allele in ALS/FTD. Nature communications, 10(1), 1-14.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Documents

Datasheet
SDS
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Second antibody and isotype control

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