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Mouse Anti-AFF4 Recombinant Antibody (V2-180054) (CBMAB-A1462-YC)

Provided herein is a Mouse monoclonal antibody against Human AF4/FMR2 Family Member 4. The antibody can be used for immunoassay techniques, such as ELISA, WB.
See all AFF4 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
V2-180054
Antibody Isotype
IgG1, κ
Application
ELISA, WB

Basic Information

Immunogen
AFF4 (NP_055238, 1 a.a. ~ 108 a.a) partial recombinant protein with GST tag.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
IHC-P1 μg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
PBS, pH 7.2
Buffer
PBS, pH7.4
Preservative
None
Concentration
Batch dependent
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
AF4/FMR2 Family Member 4
Introduction
AFF4 belongs to the AF4 family of transcription factors involved in leukemia. It is a component of the positive transcription elongation factor b (P-TEFb) complex. A chromosomal translocation involving this gene and MLL gene on chromosome 11 is found in i
Entrez Gene ID
27125
UniProt ID
Q9UHB7
Alternative Names
AF4/FMR2 Family Member 4; Major CDK9 Elongation Factor-Associated Protein; ALL1-Fused Gene From Chromosome 5q31 Protein; AF5Q31; MCEF; ALL1 Fused Gene From 5q31; AF4/FMR2 Family, Member 4; Protein AF-5q31; CHOPS;
Function
Key component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. In the SEC complex, AFF4 acts as a central scaffold that recruits other factors through direct interactions with ELL proteins (ELL, ELL2 or ELL3) and the P-TEFb complex. In case of infection by HIV-1 virus, the SEC complex is recruited by the viral Tat protein to stimulate viral gene expression.
Biological Process
Regulation of gene expression
Spermatid development
Transcription by RNA polymerase II
Transcription elongation from RNA polymerase II promoter
Cellular Location
Nucleus. Associates to transcriptionally active chromatin but not at snRNA genes.
Involvement in disease
CHOPS syndrome (CHOPS): A syndrome characterized by cognitive impairment, coarse facies, heart defects, obesity, pulmonary involvement, short stature, and skeletal dysplasia.

Hu, H., Zhang, Y., Zhao, L., Zhao, W., Wang, X., Ye, E., ... & Huang, Y. (2021). AFF4 facilitates melanoma cell progression by regulating c-Jun activity. Experimental Cell Research, 399(2), 112445.

Li, J., Ge, J., Yang, Y., Liu, B., Zheng, M., & Shi, R. (2020). Long noncoding RNA ZFPM2‐AS1 is involved in lung adenocarcinoma via miR‐511‐3p/AFF4 pathway. Journal of cellular biochemistry, 121(3), 2534-2542.

Xiao, Q., Zhang, Y., Qi, X., Chen, Y., Sheng, R., Xu, R., ... & Zhou, C. (2020). AFF4 regulates osteogenic differentiation of human dental follicle cells. International journal of oral science, 12(1), 1-11.

Gao, Q., Zheng, J., Ni, Z., Sun, P., Yang, C., Cheng, M., ... & Li, Y. (2020). The m6A Methylation-Regulated AFF4 Promotes Self-Renewal of Bladder Cancer Stem Cells. Stem cells international, 2020.

Walton, S. J., Wang, H., Quintero-Cadena, P., Bateman, A., & Sternberg, P. W. (2020). Caenorhabditis elegans AF4/FMR2 family homolog affl-2 regulates heat-shock-induced gene expression. Genetics, 215(4), 1039-1054.

Cheng, M., Sheng, L., Gao, Q., Xiong, Q., Zhang, H., Wu, M., ... & Li, Y. (2019). The m 6 A methyltransferase METTL3 promotes bladder cancer progression via AFF4/NF-κB/MYC signaling network. Oncogene, 38(19), 3667-3680.

Chen, Y., & Cramer, P. (2019). Structure of the super-elongation complex subunit AFF4 C-terminal homology domain reveals requirements for AFF homo-and heterodimerization. Journal of Biological Chemistry, 294(27), 10663-10673.

Deng, P., Wang, J., Zhang, X., Wu, X., Ji, N., Li, J., ... & Chen, Q. (2018). AFF4 promotes tumorigenesis and tumor-initiation capacity of head and neck squamous cell carcinoma cells by regulating SOX2. Carcinogenesis, 39(7), 937-947.

Zhou, C. C., Xiong, Q. C., Zhu, X. X., Du, W., Deng, P., Li, X. B., ... & Yuan, Q. (2017). AFF1 and AFF4 differentially regulate the osteogenic differentiation of human MSCs. Bone research, 5(1), 1-10.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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