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Mouse Anti-AFG1L Recombinant Antibody (V2-58426) (CBMAB-L0071-YJ)

Provided herein is a Mouse monoclonal antibody, which binds to AFG1 Like ATPase (AFG1L). The antibody can be used for immunoassay techniques, such as WB, IP, IF, ELISA.
See all AFG1L antibodies

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
V2-58426
Antibody Isotype
IgM, κ
Application
WB, IP, IF, ELISA

Basic Information

Immunogen
Epitope mapping between amino acids 424-442 near the C-terminus of LACE1 of human origin.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgM, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate).
IF(ICC)1:50-1:500
ELISA1:100-1:2,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
AFG1 Like ATPase
Introduction
AFG1L is a mitochondrial integral membrane protein that participate in mitochondrial protein homeostasis. AFG1L contains a P-loop motif and a five-domain structure that is conserved in fly, yeast, and bacteria. It functions to mediate the degradation of nuclear-encoded complex IV subunits. Two conserved estrogen receptor binding sites are located within 2.5 kb of this gene. Alternative splicing results in multiple transcript variants.
Entrez Gene ID
Human246269
Mouse215951
Rat502479
UniProt ID
HumanQ8WV93
MouseQ3V384
RatQ32PX9
Alternative Names
AFG1 Like ATPase; Lactation Elevated Protein 1; Lactation Elevated 1; LACE1; AFG1; ATPase Family Gene 1 Homolog (S. Cerevisiae); ATPase Family Gene 1 Homolog
Function
Putative mitochondrial ATPase. Plays a role in mitochondrial morphology and mitochondrial protein metabolism. Promotes degradation of excess nuclear-encoded complex IV subunits (COX4I1, COX5A and COX6A1) and normal activity of complexes III and IV of the respiratory chain. Mediates mitochondrial translocation of TP53 and its transcription-independent apoptosis in response to genotoxic stress.
Biological Process
Mitochondrial electron transport, cytochrome c to oxygen
Mitochondrial protein catabolic process
Mitochondrion organization
Cellular Location
Mitochondrion membrane
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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