Mouse Anti-AFP Monoclonal Antibody (V2-632687) (CBMAB-R0433-FY)

Name: Mouse Anti-AFP Monoclonal Antibody (V2-632687)
SKU: CBMAB-R0433-FY
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-AFP Monoclonal Antibody (CBFYR0433) (CBMAB-R0433-FY) in FC

The in vitro differentiation capacity of hiPSCs into three germ layers; the endoderm, ectoderm and mesoderm, was confirmed through embryoid body (EB) formation and positive ICC staining of the endodermal marker alpha-fetoprotein (AFP), ectodermal marker orthodenticle homolog 2 (OTX2) and mesodermal marker smooth muscle actin (SMA). ...View More

Lotila, J., Hyvärinen, T., Skottman, H., Airas, L., Narkilahti, S., & Hagman, S. (2022). Establishment of a human induced pluripotent stem cell line (TAUi008-A) derived from a multiple sclerosis patient. Stem Cell Research, 63, 102865.

Mouse Anti-AFP Monoclonal Antibody (CBFYR0433) (CBMAB-R0433-FY) in ICC

Immunofluorescence stainings showing protein expression of three germ lineages. ...View More

Banal, C., & Lefort, N. (2022). Generation and characterization of IMAGINi013-A, an induced pluripotent stem cell line generated from a healthy donor. Stem Cell Research, 61, 102755.

Mouse Anti-AFP Monoclonal Antibody (CBFYR0433) (CBMAB-R0433-FY) in ICC

hPSCs cultured for over 40 passages using the defined, xeno-free hPSC medium differentiate readily to early ectoderm, mesoderm and endoderm (EB formation).
A) Phase contrast images of the hPSC lines just prior to differentiation. B) Embryoid body formation in cultures differentiated for 14 days in culture. C) Antibodies detecting Beta-III-Tubulin (TUJ1) are shown in red; Smooth Muscle Actin (SMA) and Alpha-Feto Protein (AFP) antigens are shown in green. Nuclei were visualized using DAPI (blue). Scale bar: 200 μm. ...View More

Ahmadian Baghbaderani, B., Tian, X., Scotty Cadet, J., Shah, K., Walde, A., Tran, H., ... & Fellner, T. (2016). A newly defined and xeno-free culture medium supports every-other-day medium replacement in the generation and long-term cultivation of human pluripotent stem cells. PLoS One, 11(9), e0161229.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

V2-632687

Application

FC, IF

Immunogen

Human umbilical cord serum-derived alpha -Fetoprotein/AFP.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
IF(ICC)	8-25 μg/ml
FC	0.25 μg/10^6 cells

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Lyophilized

Buffer

PBS, Trehalose

Preservative

None

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Alpha Fetoprotein

Introduction

This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.

Entrez Gene ID

174

UniProt ID

P02771

Alternative Names

Alpha Fetoprotein; Alpha-1-Fetoprotein; Alpha-Fetoglobulin; HPAFP; Alpha-Fetoprotein; AFPD; FETA

Function

Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.

Biological Process

Cellular protein metabolic process
Post-translational protein modification

Cellular Location

Secreted

Involvement in disease

Alpha-fetoprotein deficiency (AFPD): A benign condition characterized by undetectable AFP levels in the amniotic fluid. Affected individuals are asymptomatic and present normal development.
Alpha-fetoprotein, hereditary persistence (HPAFP): A benign autosomal dominant condition characterized by continued expression of alpha-fetoprotein in adult life.

PTM

Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.
Sulfated.

Zhu, A. X., Finn, R. S., Kang, Y. K., Yen, C. J., Galle, P. R., Llovet, J. M., ... & Kudo, M. (2021). Serum alpha-fetoprotein and clinical outcomes in patients with advanced hepatocellular carcinoma treated with ramucirumab. British journal of cancer, 124(8), 1388-1397.

Zhang, J., Chen, G., Zhang, P., Zhang, J., Li, X., Gan, D. N., ... & Ye, Y. A. (2020). The threshold of alpha-fetoprotein (AFP) for the diagnosis of hepatocellular carcinoma: A systematic review and meta-analysis. PLoS One, 15(2), e0228857.

Zheng, Y., Zhu, M., & Li, M. (2020). Effects of alpha-fetoprotein on the occurrence and progression of hepatocellular carcinoma. Journal of Cancer Research and Clinical Oncology, 1-8.

He, C., Peng, W., Liu, X., Li, C., Li, X., & Wen, T. F. (2019). Post-treatment alpha-fetoprotein response predicts prognosis of patients with hepatocellular carcinoma: a meta-analysis. Medicine, 98(31).

Galle, P. R., Foerster, F., Kudo, M., Chan, S. L., Llovet, J. M., Qin, S., ... & Zhu, A. X. (2019). Biology and significance of alpha‐fetoprotein in hepatocellular carcinoma. Liver international, 39(12), 2214-2229.

Mehta, N., Dodge, J. L., Roberts, J. P., Hirose, R., & Yao, F. Y. (2019). Alpha‐fetoprotein decrease from> 1,000 to< 500 ng/mL in patients with hepatocellular carcinoma leads to improved posttransplant outcomes. Hepatology, 69(3), 1193-1205.

Chan, M. Y., She, W. H., Dai, W. C., Tsang, S. H. Y., Chok, K. S. H., Chan, A. C. Y., ... & Cheung, T. T. (2019). Prognostic value of preoperative alpha-fetoprotein (AFP) level in patients receiving curative hepatectomy-an analysis of 1,182 patients in Hong Kong. Translational gastroenterology and hepatology, 4.

Tzartzeva, K., Obi, J., Rich, N. E., Parikh, N. D., Marrero, J. A., Yopp, A., ... & Singal, A. G. (2018). Surveillance imaging and alpha fetoprotein for early detection of hepatocellular carcinoma in patients with cirrhosis: a meta-analysis. Gastroenterology, 154(6), 1706-1718.

Carr, B. I., Akkiz, H., Üsküdar, O., Yalçın, K., Guerra, V., Kuran, S., ... & Tokat, Y. (2018). HCC with low-and normal-serum alpha-fetoprotein levels. Clinical practice (London, England), 15(1), 453.

Li, G., Li, S., Wang, Z., Xue, Y., Dong, C., Zeng, J., ... & Zhou, Z. (2018). Label-free electrochemical aptasensor for detection of alpha-fetoprotein based on AFP-aptamer and thionin/reduced graphene oxide/gold nanoparticles. Analytical biochemistry, 547, 37-44.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Antibody Pairs

AFP Matched Antibody Pair (030) (CAT#: APMAB-030LY)

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Isotype control

For research use only. Not intended for any clinical use.

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