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Mouse Anti-AGO2 Recombinant Antibody (V2-613109) (CBMAB-A2522-LY)

The product is antibody recognizes EIF2C2. The antibody 2E12-1C9 immunoassay techniques such as: WB, ELISA.
See all AGO2 antibodies
Published Data
Mouse Anti-AGO2 Recombinant Antibody (2E12-1C9) (CBMAB-A2522-LY) in WB
Efficient AGO2 depletion in PCNs. Ago2 expression levels in Ago2-depleted and control scrambled shRNA samples were quantified by RT-qPCR, normalized to Gapdh, and plotted on y as individual biological triplicates (colored dots, n = 3). P value was computed by two-sided t-test. Western blotting against AGO2 and ACTB (loading control) is shown below the plot. b, Ago2 knockdown shifts N-zip reporters with let-7 sites toward soma. Box plots showing changes in neurite/soma enrichment between Ago2-depleted and scrambled samples (y) as a function of the number of let-7 sites in the N-zip reporter tiles (x). P values were computed using two-sided Wilcoxon rank-sum test (n = 3 independent biological replicates). ...View More
Mendonsa, S., von Kügelgen, N., Dantsuji, S., Ron, M., Breimann, L., Baranovskii, A., ... & Chekulaeva, M. (2023). Massively parallel identification of mRNA localization elements in primary cortical neurons. Nature Neuroscience, 1-12.
Mouse Anti-AGO2 Recombinant Antibody (2E12-1C9) (CBMAB-A2522-LY) in IP
HBV-miR-6 interacts with RICS. RNA immunoprecipitation using either an AGO2, NRNP70 (positive control), or IgG control antibodies was performed in HepG2.2.15 cells (black bars) and HepG2 cells (grey bars). The binding of HBV-miR-6 (A), HBV-miR-7 (B), and HBV-miR-8 (C) to AGO2, and U1 snRNA to SNRNP70, were measured using RT-qPCR. The graphs represent averages of results from two independent experiments. ...View More
Loukachov, V., van Dort, K. A., Jansen, L., Reesink, H. W., & Kootstra, N. A. (2022). Identification of a novel HBV encoded miRNA using next generation sequencing. Viruses, 14(6), 1223.
Mouse Anti-AGO2 Recombinant Antibody (2E12-1C9) (CBMAB-A2522-LY) in WB
Cellular levels of miRNP-associated proteins in anti-miR-146a- and control anti-miR-122-transfected LPS-treated macrophages. miRNP-associated proteins and processing enzymes Dicer1, Ago2, TRBP2, and Drosha were Western blotted in miR-146a-5p-inactivated LPS-treated RAW264.7 cells. β-Actin served as an endogenous control. 18s rRNA or GAPDH and U6 snRNA were used as endogenous targets in qRT-PCR of mRNA and miRNA quantification, respectively. ...View More
Chatterjee, S., Mukherjee, I., Bhattacharjee, S., Bose, M., Chakrabarti, S., & Bhattacharyya, S. N. (2022). Target-Dependent Coordinated Biogenesis of Secondary MicroRNAs by miR-146a Balances Macrophage Activation Processes. Molecular and Cellular Biology, 42(4), e00452-21.
Mouse Anti-AGO2 Recombinant Antibody (2E12-1C9) (CBMAB-A2522-LY) in WB and IP
The lysates of HeLa cells that were transiently transfected with pCK-FLAG-Ago2 or empty vector were subjected to IP using anti-FLAG antibodies in the presence (+) or absence (−) of RNaseA. Western blotting was performed to detect proteins in IP inputs and eluates. ...View More
Park, J., Seo, J. W., Ahn, N., Park, S., Hwang, J., & Nam, J. W. (2019). UPF1/SMG7-dependent microRNA-mediated gene regulation. Nature communications, 10(1), 4181.

Summary

Host Animal
Mouse
Specificity
Human
Clone
V2-613109
Antibody Isotype
IgG1, κ
Application
WB, ELISA, IHC-P, IF

Basic Information

Immunogen
EIF2C2 (AAH07633.1, 1 a.a. ~ 377 a.a) full-length recombinant protein with GST tag.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
IHC-P3 μg/ml
IF(ICC)10 μg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.4
Preservative
None
Concentration
Batch dependent
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Argonaute 2, RISC Catalytic Component
Introduction
This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
Entrez Gene ID
27161
UniProt ID
Q9UKV8
Alternative Names
AGO2; MGC3183; Q10
Function
Required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). The 'minimal RISC' appears to include AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA). These guide RNAs direct RISC to complementary mRNAs that are targets for RISC-mediated gene silencing. The precise mechanism of gene silencing depends on the degree of complementarity between the miRNA or siRNA and its target. Binding of RISC to a perfectly complementary mRNA generally results in silencing due to endonucleolytic cleavage of the mRNA specifically by AGO2. Binding of RISC to a partially complementary mRNA results in silencing through inhibition of translation, and this is independent of endonuclease activity. May inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the translation initiation factor eIF4-E. May also inhibit translation initiation via interaction with EIF6, which itself binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit. The inhibition of translational initiation leads to the accumulation of the affected mRNA in cytoplasmic processing bodies (P-bodies), where mRNA degradation may subsequently occur. In some cases RISC-mediated translational repression is also observed for miRNAs that perfectly match the 3' untranslated region (3'-UTR). Can also up-regulate the translation of specific mRNAs under certain growth conditions. Binds to the AU element of the 3'-UTR of the TNF (TNF-alpha) mRNA and up-regulates translation under conditions of serum starvation. Also required for transcriptional gene silencing (TGS), in which short RNAs known as antigene RNAs or agRNAs direct the transcriptional repression of complementary promoter regions.
Biological Process
Gene silencing by RNA
Intracellular receptor signaling pathway
miRNA loading onto RISC involved in gene silencing by miRNA
miRNA mediated inhibition of translation
miRNA metabolic process
mRNA cleavage involved in gene silencing by miRNA
mRNA cleavage involved in gene silencing by siRNA
Negative regulation of amyloid precursor protein biosynthetic process
Negative regulation of gene expression
Negative regulation of translational initiation
Positive regulation of angiogenesis
Positive regulation of gene expression
Positive regulation of miRNA mediated inhibition of translation
Positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay
Positive regulation of nuclear-transcribed mRNA poly(A) tail shortening
Positive regulation of transcription by RNA polymerase II
Positive regulation of translation, ncRNA-mediated
Positive regulation of trophoblast cell migration
Post-embryonic development
Post-transcriptional gene silencing by RNA
Pre-miRNA processing
Production of miRNAs involved in gene silencing by miRNA
Production of siRNA involved in RNA interference
Regulation of gene silencing by miRNA
RNA secondary structure unwinding
siRNA loading onto RISC involved in RNA interference
Translation
Wnt signaling pathway, calcium modulating pathway
Cellular Location
Nucleus; P-body. Translational repression of mRNAs results in their recruitment to P-bodies. Translocation to the nucleus requires IMP8.
PTM
Hydroxylated. 4-hydroxylation appears to enhance protein stability but is not required for miRNA-binding or endonuclease activity.
Ubiquitinated on surface-exposed lysines by a SCF-like E3 ubiquitin-protein ligase complex containing ZSWIM8 during target-directed microRNA degradation (TDMD), a process that mediates degradation of microRNAs (miRNAs). Ubiquitination by the SCF-like E3 ubiquitin-protein ligase complex containing ZSWIM8 leads to its subsequent degradation, thereby exposing miRNAs for degradation. ZSWIM8 recognizes and binds AGO2 when it is engaged with a TDMD target.
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For research use only. Not intended for any clinical use.

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