Mouse Anti-AGO4 Recombinant Antibody (V2-613112) (CBMAB-A2525-LY)

Name: Mouse Anti-AGO4 Recombinant Antibody (V2-613112)
SKU: CBMAB-A2525-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

V2-613112

Application

SELISA, ELISA

Immunogen

EIF2C4 (NP_060099.2, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4

Preservative

None

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Argonaute 4, RISC Catalytic Component

Introduction

This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic containing PAZ and PIWI domains, and it may play a role in short-interfering-RNA-mediated gene silencing. This gene is located on chromosome 1 in a cluster of closely related family members including argonaute 3, and eukaryotic translation initiation factor 2C, 1. [provided by RefSeq]

Entrez Gene ID

192670

UniProt ID

Q9HCK5

Alternative Names

AGO4

Function

Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Also required for RNA-directed transcription and replication of the human hapatitis delta virus (HDV).

Biological Process

Intracellular receptor signaling pathway
Male gonad development
Male meiotic nuclear division
miRNA loading onto RISC involved in gene silencing by miRNA
miRNA mediated inhibition of translation
miRNA metabolic process
mRNA catabolic process
Negative regulation of apoptotic process
Negative regulation of gene expression
Positive regulation of gene expression
Post-transcriptional gene silencing by RNA
Pre-miRNA processing
Production of miRNAs involved in gene silencing by miRNA
Regulation of cell morphogenesis
Regulation of megakaryocyte differentiation
Regulation of transcription initiation from RNA polymerase II promoter
RNA secondary structure unwinding
Synaptonemal complex assembly
Wnt signaling pathway, calcium modulating pathway

Cellular Location

P-body

PTM

Ubiquitinated on surface-exposed lysines by a SCF-like E3 ubiquitin-protein ligase complex containing ZSWIM8 during target-directed microRNA degradation (TDMD), a process that mediates degradation of microRNAs (miRNAs). Ubiquitination by the SCF-like E3 ubiquitin-protein ligase complex containing ZSWIM8 leads to its subsequent degradation, thereby exposing miRNAs for degradation. ZSWIM8 recognizes and binds AGO4 when it is engaged with a TDMD target.

Nowak, I., & Sarshad, A. A. (2021). Argonaute Proteins Take Center Stage in Cancers. Cancers, 13(4), 788.

Kobayashi, H., Shoji, K., Kiyokawa, K., Negishi, L., & Tomari, Y. (2019). VCP machinery mediates autophagic degradation of empty Argonaute. Cell reports, 28(5), 1144-1153.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Mouse Anti-AGO4 Recombinant Antibody (V2-180181) (CAT#: CBMAB-A1602-YC)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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