Sign in or Register   Sign in or Register
  |  

Rabbit Anti-AGO4 Recombinant Antibody (V2-180180) (CBMAB-A1601-YC)

Provided herein is a Rabbit monoclonal antibody against Human Argonaute 4, RISC Catalytic Component. The antibody can be used for immunoassay techniques, such as WB, IP.
See all AGO4 antibodies
Published Data
Rabbit Anti-AGO4 Recombinant Antibody (D10F10) (CBMAB-A1601-YC) in IP
AGO2 binds to 32P-labeled miR-9-5p and miR-9-3p. Representative gel image of 32P-labeled miR-9-5p, miR-9-3p, and an equimolar equivalent mixture of both following immunoprecipitation with AGO4 and β-actin. ...View More
Kim, C. K., Asimes, A., Zhang, M., Son, B. T., Kirk, J. A., & Pak, T. R. (2020). Differential stability of miR-9-5p and miR-9-3p in the brain is determined by their unique cis-and trans-acting elements. Eneuro, 7(3).
Rabbit Anti-AGO4 Recombinant Antibody (D10F10) (CBMAB-A1601-YC) in WB
Western blot and densitometric analyses showing α1A-Q33-FLAG and α1ACT-Q33-FLAG expression in HEK293 cells treated with four siRNAs or si-C in the presence of miR-3191-5p or NC (n = 6). ...View More
Miyazaki, Y., Du, X., Muramatsu, S. I., & Gomez, C. M. (2016). An miRNA-mediated therapy for SCA6 blocks IRES-driven translation of the CACNA1A second cistron. Science translational medicine, 8(347), 347ra94-347ra94.

Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat, Monkey
Clone
V2-180180
Antibody Isotype
IgG
Application
WB, IP

Basic Information

Immunogen
Synthetic peptide corresponding to residues near the amino terminus of human Argonaute 4 protein.
Host Species
Rabbit
Specificity
Human, Mouse, Rat, Monkey
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:1,000
IP1:50

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
HEPES, pH 7.5, 100 µg/ml BSA, 50% glycerol
Preservative
0.02% sodium azide
Concentration
Batch dependent
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Argonaute 4, RISC Catalytic Component
Introduction
AGO4 is a member of the Argonaute family of proteins which contain PAZ and PIWI domains and play an integral role in RNA interference and short-interfering-RNA-mediated gene silencing. This gene is located on chromosome 1 in a cluster of related family me
Entrez Gene ID
Human192670
Mouse76850
Rat298533
Monkey713134
UniProt ID
HumanQ9HCK5
MouseQ8CJF8
RatF1LUQ5
MonkeyF7EGT1
Alternative Names
Argonaute 4, RISC Catalytic Component; Eukaryotic Translation Initiation Factor 2C, 4; Argonaute RISC Catalytic Component 4; EIF2C4; Eukaryotic Translation Initiation Factor 2C 4; Protein Argonaute-4; Argonaute 4;
Function
Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Also required for RNA-directed transcription and replication of the human hapatitis delta virus (HDV).
Biological Process
Intracellular receptor signaling pathway
Male gonad development
Male meiotic nuclear division
miRNA loading onto RISC involved in gene silencing by miRNA
miRNA mediated inhibition of translation
miRNA metabolic process
mRNA catabolic process
Negative regulation of apoptotic process
Negative regulation of gene expression
Positive regulation of gene expression
Post-transcriptional gene silencing by RNA
Pre-miRNA processing
Production of miRNAs involved in gene silencing by miRNA
Regulation of cell morphogenesis
Regulation of megakaryocyte differentiation
Regulation of transcription initiation from RNA polymerase II promoter
RNA secondary structure unwinding
Synaptonemal complex assembly
Wnt signaling pathway, calcium modulating pathway
Cellular Location
P-body
PTM
Ubiquitinated on surface-exposed lysines by a SCF-like E3 ubiquitin-protein ligase complex containing ZSWIM8 during target-directed microRNA degradation (TDMD), a process that mediates degradation of microRNAs (miRNAs). Ubiquitination by the SCF-like E3 ubiquitin-protein ligase complex containing ZSWIM8 leads to its subsequent degradation, thereby exposing miRNAs for degradation. ZSWIM8 recognizes and binds AGO4 when it is engaged with a TDMD target.
Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Rabbit Anti-AGO4 Recombinant Antibody (V2-180180)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Go to
Compare