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Mouse Anti-AGTRAP Recombinant Antibody (V2-180252) (CBMAB-A1685-YC)

Provided herein is a Mouse monoclonal antibody against Human Angiotensin II Receptor Associated Protein. The antibody can be used for immunoassay techniques, such as ELISA, IHC, WB.
See all AGTRAP antibodies
Published Data
Mouse Anti-AGTRAP Recombinant Antibody (CBYC-A435) (CBMAB-A1685-YC) in WB
Western blot and subsequent quantification of ATRAP, p-AKT, AKT, TGF-β, p-Smad2/3, Smad2, Smad3, and GAPDH in the atrial tissues of saline- or Ang II-infused mice with or without melatonin (n = 4 mice per group). *P < 0.05, **P < 0.01 versus saline treatment; #P < 0.05, ##P < 0.01 versus Ang II treatment. ...View More
Xie, X., Shen, T. T., Bi, H. L., Su, Z. L., Liao, Z. Q., Zhang, Y., ... & Xia, Y. L. (2022). Melatonin inhibits angiotensin II-induced atrial fibrillation through preventing degradation of Ang II Type I Receptor-Associated Protein (ATRAP). Biochemical Pharmacology, 202, 115146.
Mouse Anti-AGTRAP Recombinant Antibody (CBYC-A435) (CBMAB-A1685-YC) in IP
The lysates from atria were immunoprecipitated with anti-ATRAP, and ubiquitinantion of ATRAP was evaluated by anti-ubiquitin and anti-ATRAP (n = 3). ...View More
Xie, X., Shen, T. T., Bi, H. L., Su, Z. L., Liao, Z. Q., Zhang, Y., ... & Xia, Y. L. (2022). Melatonin inhibits angiotensin II-induced atrial fibrillation through preventing degradation of Ang II Type I Receptor-Associated Protein (ATRAP). Biochemical Pharmacology, 202, 115146.
Mouse Anti-AGTRAP Recombinant Antibody (CBYC-A435) (CBMAB-A1685-YC) in WB
Oxidative stress induced by H2O2 damage induced the upregulation of vascular disease-associated protein expression in HUVECs compared with the control group. Compared with the control group, the relative expression of angiotensinogen, endothelin-1, ATRAP and PDGF in the H2O2 group was 2.10±0.097/1.48±0.112, 1.66±0.063/0.78±0.065, 1.28±0.068/0.80±0.023 and 1.33±0.031/0.63±0.020, respectively (*P<0.001). ...View More
Wang, Z., Wang, Y., Chen, Y., & Lv, J. (2016). The IL-24 gene protects human umbilical vein endothelial cells against H2O2-induced injury and may be useful as a treatment for cardiovascular disease. International Journal of Molecular Medicine, 37(3), 581-592.

Summary

Host Animal
Mouse
Specificity
Human
Clone
V2-180252
Antibody Isotype
IgG1, κ
Application
ELISA, IHC, WB, IP

Basic Information

Immunogen
Amino acids 1-159 representing full length AGTRAP of human origin.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate).
IF(ICC)1:50-1:500
ELISA1:100-1:2,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid in PBS, pH 7.2
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Angiotensin II Receptor Associated Protein
Introduction
AGTRAP is a transmembrane protein localized to the plasma membrane and perinuclear vesicular structures. The gene product interacts with the angiotensin II type I receptor and negatively regulates angiotensin II signaling.
Entrez Gene ID
57085
UniProt ID
Q6RW13
Alternative Names
Angiotensin II Receptor Associated Protein; AT1 Receptor-Associated Protein; ATRAP; Angiotensin II, Type I Receptor-Associated Protein; Type-1 Angiotensin II Receptor-Associated Protein; ATI Receptor-Associated Protein;
Function
Appears to be a negative regulator of type-1 angiotensin II receptor-mediated signaling by regulating receptor internalisation as well as mechanism of receptor desensitization such as phosphorylation. Induces also a decrease in cell proliferation and angiotensin II-stimulated transcriptional activity.
Biological Process
regulation of blood pressure Source: GO_Central
response to hypoxia
Cellular Location
Golgi apparatus membrane; Endoplasmic reticulum membrane; Cytoplasmic vesicle membrane. Present in perinuclear vesicular membranes, Endoplasmic reticulum, Golgi and endocytic vesicles.
Topology
Extracellular: 1-23 aa
Helical: 24-44 aa
Cytoplasmic: 45-55 aa
Helical: 56-76 aa
Extracellular: 77-86 aa
Helical: 87-107 aa
Cytoplasmic: 108-159 aa

Polidoro, J. Z., Rebouças, N. A., & Girardi, A. C. C. (2021). The Angiotensin II Type 1 Receptor-Associated Protein Attenuates Angiotensin II-Mediated Inhibition of the Renal Outer Medullary Potassium Channel in Collecting Duct Cells. Frontiers in physiology, 12.

Yamaji, T., Yamashita, A., Wakui, H., Azushima, K., Uneda, K., Fujikawa, Y., ... & Tamura, K. (2019). Angiotensin II type 1 receptor-associated protein deficiency attenuates sirtuin1 expression in an immortalised human renal proximal tubule cell line. Scientific reports, 9(1), 1-11.

Kinguchi, S., Wakui, H., Azushima, K., Haruhara, K., Koguchi, T., Ohki, K., ... & Tamura, K. (2019). Effects of ATRAP in Renal Proximal Tubules on Angiotensin‐Dependent Hypertension. Journal of the American Heart Association, 8(8), e012395.

Xian, Y., Dong, L., Jia, Y., Lin, Y., Jiao, W., & Wang, Y. (2018). miR‐370 promotes high glucose‐induced podocyte injuries by inhibiting angiotensin II type 1 receptor‐associated protein. Cell biology international, 42(11), 1545-1555.

Uneda, K., Wakui, H., Maeda, A., Azushima, K., Kobayashi, R., Haku, S., ... & Tamura, K. (2017). Angiotensin II Type 1 receptor‐associated protein regulates kidney aging and lifespan independent of angiotensin. Journal of the American Heart Association, 6(8), e006120.

Ohki, K., Wakui, H., Kishio, N., Azushima, K., Uneda, K., Haku, S., ... & Tamura, K. (2018). Angiotensin II type 1 receptor-associated protein inhibits angiotensin II-induced insulin resistance with suppression of oxidative stress in skeletal muscle tissue. Scientific reports, 8(1), 1-12.

Azushima, K., Ohki, K., Wakui, H., Uneda, K., Haku, S., Kobayashi, R., ... & Tamura, K. (2017). Adipocyte‐specific enhancement of angiotensin II type 1 receptor‐associated protein ameliorates diet‐induced visceral obesity and insulin resistance. Journal of the American Heart Association, 6(3), e004488.

Luo, C., Lv, N., Chang, Z., Qu, Q., & Huang, J. (2017). Adipose angiotensin II type 1 receptor-associated protein ameliorates metabolic disorders via promoting adipose tissue adipogenesis and browning. European journal of cell biology, 96(6), 567-578.

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For research use only. Not intended for any clinical use.

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