Sign in or Register   Sign in or Register
  |  

Mouse Anti-AICDA Recombinant Antibody (10B92) (CBMAB-A1774-YC)

Provided herein is a Mouse monoclonal antibody against Human Activation Induced Cytidine Deaminase. The antibody can be used for immunoassay techniques, such as ELISA, WB.
See all AICDA antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
V2-180317
Antibody Isotype
IgG1
Application
WB, IHC-P

Basic Information

Immunogen
A recombinant protein of full length human AID.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB0.1-1 μg/ml
IHC-P2-10 μg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid in PBS, pH 7.4, 0.09% sodium azide
Buffer
PBS
Preservative
0.01% sodium azide
Concentration
0.5 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Activation Induced Cytidine Deaminase
Introduction
AICDA is a RNA-editing deaminase that is a member of the cytidine deaminase family. The protein is involved in somatic hypermutation, gene conversion, and class-switch recombination of immunoglobulin genes. Defects in this gene are the cause of autosomal
Entrez Gene ID
57379
UniProt ID
Q9GZX7
Alternative Names
Activation Induced Cytidine Deaminase; Activation-Induced Cytidine Deaminase; Cytidine Aminohydrolase; AID; Integrated Into Burkitts Lymphoma Cell Line Ramos; Single-Stranded DNA Cytosine Deaminase; Epididymis Secretory Protein Li 284;
Function
Single-stranded DNA-specific cytidine deaminase. Involved in somatic hypermutation (SHM), gene conversion, and class-switch recombination (CSR) in B-lymphocytes by deaminating C to U during transcription of Ig-variable (V) and Ig-switch (S) region DNA. Required for several crucial steps of B-cell terminal differentiation necessary for efficient antibody responses (PubMed:18722174, PubMed:21385873, PubMed:21518874, PubMed:27716525). May also play a role in the epigenetic regulation of gene expression by participating in DNA demethylation (PubMed:21496894).
Biological Process
B cell differentiation Source: UniProtKB
Cytidine to uridine editing Source: GO_Central
Defense response to virus Source: GO_Central
DNA cytosine deamination Source: GO_Central
DNA demethylation Source: UniProtKB
mRNA processing Source: UniProtKB-KW
Negative regulation of DNA methylation-dependent heterochromatin assembly Source: UniProtKB
Negative regulation of single stranded viral RNA replication via double stranded DNA intermediate Source: GO_Central
Negative regulation of transposition Source: GO_Central
Regulation of nuclear cell cycle DNA replication Source: UniProtKB
Somatic diversification of immunoglobulins Source: UniProtKB
Somatic hypermutation of immunoglobulin genes
Cellular Location
Cytoplasm; Nucleus. Predominantly cytoplasmic (PubMed:21385873). In the presence of MCM3AP/GANP, relocalizes to the nucleus (By similarity).
Involvement in disease
Immunodeficiency with hyper-IgM 2 (HIGM2): A rare immunodeficiency syndrome characterized by normal or elevated serum IgM levels with absence of IgG, IgA, and IgE. It results in a profound susceptibility to bacterial infections.
PTM
Ser-38 is the major site whereas Thr-27 is the minor site of phosphorylation. Phosphorylation regulates its class-switch recombination activity.2 Publications
Probably monoubiquitinated on several residues by RNF126.

Akbay, B., Germini, D., Bissenbaev, A. K., Musinova, Y. R., Sheval, E. V., Vassetzky, Y., & Dokudovskaya, S. (2021). HIV-1 Tat Activates Akt/mTORC1 Pathway and AICDA Expression by Downregulating Its Transcriptional Inhibitors in B Cells. International journal of molecular sciences, 22(4), 1588.

Mdletshe, N., Nel, A., Shires, K., & Mowla, S. (2020). HIV Nef enhances the expression of oncogenic c-MYC and activation-induced cytidine deaminase in Burkitt lymphoma cells, promoting genomic instability. Infectious Agents and Cancer, 15(1), 1-10.

Hale, L. P. (2020). Deficiency of activation-induced cytidine deaminase in a murine model of ulcerative colitis. Plos one, 15(9), e0239295.

Lio, C. W. J., Shukla, V., Samaniego-Castruita, D., González-Avalos, E., Chakraborty, A., Yue, X., ... & Rao, A. (2019). TET enzymes augment activation-induced deaminase (AID) expression via 5-hydroxymethylcytosine modifications at the Aicda superenhancer. Science immunology, 4(34).

Abdullah, A. M., Elnasri, H. A., Albkrye, A. M., Khaeir, M. A., & Mohmed, H. O. (2019). IN–SILICO ANALYSIS OF NON-SYNONYMOUS SINGLE NUCLEOTIDE POLYMORPHISMS OF ACTIVATION-INDUCED CYTIDINE DEAMINASE (AICDA) GENE. European Journal of Biomedical, 6(11), 30-37.

Kumar, R., & Evans, T. (2019). Activation‐Induced Cytidine Deaminase Regulates Fibroblast Growth Factor/Extracellular Signal‐Regulated Kinases Signaling to Achieve the Naïve Pluripotent State During Reprogramming. Stem Cells, 37(8), 1003-1017.

Burack, R., Li, H., Adlowitz, D. G., Spence, J. M., Leblanc, M. L., Rimsza, L. M., ... & Friedberg, J. W. (2019). Intraclonal Heterogeneity Caused By Activation-Induced Cytidine Deaminase Is Not a Prognostic Biomarker in Untreated Advanced Stage Follicular Lymphoma: An Analysis of SWOG S0016.

Li, Y., Wang, Y., Sun, N., Yang, H., Zhang, Q., Zhang, X., ... & Jia, X. (2019). Activation-induced cytidine deaminase plays crucial role in ovalbumin-induced food allergy and promoted by IL-21. Molecular immunology, 114, 369-377.

Sall, F. B., El Amine, R., Markozashvili, D., Tsfasman, T., Oksenhendler, E., Lipinski, M., ... & Germini, D. (2019). HIV‐1 Tat protein induces aberrant activation of AICDA in human B‐lymphocytes from peripheral blood. Journal of cellular physiology, 234(9), 15678-15685.

El Kadi, N., Wang, L., Davis, A., Korkaya, H., Cooke, A., Vadnala, V., ... & Hassan, K. A. (2018). The EGFR T790M mutation is acquired through AICDA-mediated deamination of 5-methylcytosine following TKI treatment in lung cancer. Cancer research, 78(24), 6728-6735.

Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-AICDA Recombinant Antibody (10B92)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Go to
Compare