Mouse Anti-AICDA Recombinant Antibody (V2-180331) (CBMAB-A1788-YC)

Name: Mouse Anti-AICDA Recombinant Antibody (V2-180331)
SKU: CBMAB-A1788-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

V2-180331

Application

ELISA, IF, IHC-P, IP, WB

Immunogen

Synthetic peptide derived from the C-terminal region of mouse and rat AID

Host Species

Mouse

Specificity

Human, Mouse

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	2 μg/ml
IHC-P	1:20

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

PBS, pH 7.4, 50% glycerol, 0.02% sodium azide

Buffer

PBS, pH 7.4

Preservative

0.1% sodium azide

Concentration

0.5 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Activation Induced Cytidine Deaminase

Introduction

AICDA is a RNA-editing deaminase that is a member of the cytidine deaminase family. The protein is involved in somatic hypermutation, gene conversion, and class-switch recombination of immunoglobulin genes. Defects in this gene are the cause of autosomal

Entrez Gene ID

Human	57379
Mouse	11628

UniProt ID

Human	Q9GZX7
Mouse	Q9WVE0

Alternative Names

CDA2; HIGM2; Arp2; Aid; AID; HEL-S-284; ARP2

Function

Single-stranded DNA-specific cytidine deaminase. Involved in somatic hypermutation (SHM), gene conversion, and class-switch recombination (CSR) in B-lymphocytes by deaminating C to U during transcription of Ig-variable (V) and Ig-switch (S) region DNA. Required for several crucial steps of B-cell terminal differentiation necessary for efficient antibody responses (PubMed:18722174, PubMed:21385873, PubMed:21518874, PubMed:27716525). May also play a role in the epigenetic regulation of gene expression by participating in DNA demethylation (PubMed:21496894).

Biological Process

B cell differentiation Source: UniProtKB
Cytidine to uridine editing Source: GO_Central
Defense response to virus Source: GO_Central
DNA cytosine deamination Source: GO_Central
DNA demethylation Source: UniProtKB
mRNA processing Source: UniProtKB-KW
Negative regulation of DNA methylation-dependent heterochromatin assembly Source: UniProtKB
Negative regulation of single stranded viral RNA replication via double stranded DNA intermediate Source: GO_Central
Negative regulation of transposition Source: GO_Central
Regulation of nuclear cell cycle DNA replication Source: UniProtKB
Somatic diversification of immunoglobulins Source: UniProtKB
Somatic hypermutation of immunoglobulin genes

Cellular Location

Cytoplasm; Nucleus. Predominantly cytoplasmic (PubMed:21385873). In the presence of MCM3AP/GANP, relocalizes to the nucleus (By similarity).

Involvement in disease

Immunodeficiency with hyper-IgM 2 (HIGM2): A rare immunodeficiency syndrome characterized by normal or elevated serum IgM levels with absence of IgG, IgA, and IgE. It results in a profound susceptibility to bacterial infections.

PTM

Ser-38 is the major site whereas Thr-27 is the minor site of phosphorylation. Phosphorylation regulates its class-switch recombination activity.2 Publications
Probably monoubiquitinated on several residues by RNF126.

Akbay, B., Germini, D., Bissenbaev, A. K., Musinova, Y. R., Sheval, E. V., Vassetzky, Y., & Dokudovskaya, S. (2021). HIV-1 Tat Activates Akt/mTORC1 Pathway and AICDA Expression by Downregulating Its Transcriptional Inhibitors in B Cells. International journal of molecular sciences, 22(4), 1588.

Mdletshe, N., Nel, A., Shires, K., & Mowla, S. (2020). HIV Nef enhances the expression of oncogenic c-MYC and activation-induced cytidine deaminase in Burkitt lymphoma cells, promoting genomic instability. Infectious Agents and Cancer, 15(1), 1-10.

Hale, L. P. (2020). Deficiency of activation-induced cytidine deaminase in a murine model of ulcerative colitis. Plos one, 15(9), e0239295.

Lio, C. W. J., Shukla, V., Samaniego-Castruita, D., González-Avalos, E., Chakraborty, A., Yue, X., ... & Rao, A. (2019). TET enzymes augment activation-induced deaminase (AID) expression via 5-hydroxymethylcytosine modifications at the Aicda superenhancer. Science immunology, 4(34).

Abdullah, A. M., Elnasri, H. A., Albkrye, A. M., Khaeir, M. A., & Mohmed, H. O. (2019). IN–SILICO ANALYSIS OF NON-SYNONYMOUS SINGLE NUCLEOTIDE POLYMORPHISMS OF ACTIVATION-INDUCED CYTIDINE DEAMINASE (AICDA) GENE. European Journal of Biomedical, 6(11), 30-37.

Kumar, R., & Evans, T. (2019). Activation‐Induced Cytidine Deaminase Regulates Fibroblast Growth Factor/Extracellular Signal‐Regulated Kinases Signaling to Achieve the Naïve Pluripotent State During Reprogramming. Stem Cells, 37(8), 1003-1017.

Burack, R., Li, H., Adlowitz, D. G., Spence, J. M., Leblanc, M. L., Rimsza, L. M., ... & Friedberg, J. W. (2019). Intraclonal Heterogeneity Caused By Activation-Induced Cytidine Deaminase Is Not a Prognostic Biomarker in Untreated Advanced Stage Follicular Lymphoma: An Analysis of SWOG S0016.

Li, Y., Wang, Y., Sun, N., Yang, H., Zhang, Q., Zhang, X., ... & Jia, X. (2019). Activation-induced cytidine deaminase plays crucial role in ovalbumin-induced food allergy and promoted by IL-21. Molecular immunology, 114, 369-377.

Sall, F. B., El Amine, R., Markozashvili, D., Tsfasman, T., Oksenhendler, E., Lipinski, M., ... & Germini, D. (2019). HIV‐1 Tat protein induces aberrant activation of AICDA in human B‐lymphocytes from peripheral blood. Journal of cellular physiology, 234(9), 15678-15685.

El Kadi, N., Wang, L., Davis, A., Korkaya, H., Cooke, A., Vadnala, V., ... & Hassan, K. A. (2018). The EGFR T790M mutation is acquired through AICDA-mediated deamination of 5-methylcytosine following TKI treatment in lung cancer. Cancer research, 78(24), 6728-6735.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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