Mouse Anti-AIFM2 Recombinant Antibody (V2-356448) (CBMAB-P0032-YC)

Name: Mouse Anti-AIFM2 Recombinant Antibody (V2-356448)
SKU: CBMAB-P0032-YC
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-AIFM2 Recombinant Antibody (Pab122) in IHC

Phase-contrast microscope images of cell morphology of FSP1 DNA vector and insert transfected ARPE-19 cells. Scale bar = 100 µm. RFP red fluorescence protein, indicating successfully transfected cells. ...View More

Yang, M., Tsui, M. G., Tsang, J. K. W., Goit, R. K., Yao, K. M., So, K. F., ... & Lo, A. C. Y. (2022). Involvement of FSP1-CoQ10-NADH and GSH-GPx-4 pathways in retinal pigment epithelium ferroptosis. Cell death & disease, 13(5), 468.

Mouse Anti-AIFM2 Recombinant Antibody (Pab122) in WB

Western blot assay of the protein expression level of FSP1 in FSP1 DNA vector and insert transfected ARPE-19 cells. n = 3 (independent experiments). ...View More

Mouse Anti-AIFM2 Recombinant Antibody (Pab122) in WB

Western blot analyses and relative quantifications of FSP1 protein levels in a panel of melanoma cell lines, normalized to protein level in melanocytes (HEMa-LP). 1-way ANOVA with Dunnett's multiple comparisons test (HEMa-LP as a control) was used to quantify statistical significance. Error bars represent SEM, n ≥ 4. ...View More

Arslanbaeva, L., Tosi, G., Ravazzolo, M., Simonato, M., Tucci, F. A., Pece, S., ... & Santoro, M. M. (2022). UBIAD1 and CoQ10 protect melanoma cells from lipid peroxidation-mediated cell death. Redox Biology, 51, 102272.

Mouse Anti-AIFM2 Recombinant Antibody (Pab122) in WB

Western blot of GPD2, DHODH, FSP1, and GPX4 protein levels in HCT116 cells with the indicated genotypes. ...View More

Ma, P. W., Wang, W. L., Chen, J. W., Yuan, H., Lu, P. H., Gao, W., ... & Lu, L. J. (2022). Treatment with the ferroptosis inhibitor ferrostatin-1 attenuates noise-induced hearing loss by suppressing ferroptosis and apoptosis. Oxidative Medicine and Cellular Longevity, 2022.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

V2-356448

Application

IP, WB, IF, ELISA

Immunogen

Amino acids 1-300 mapping at the N-terminus of AMID of human origin.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:30-1:3,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Apoptosis Inducing Factor, Mitochondria Associated 2

Introduction

AIFM2 is a flavoprotein oxidoreductase that binds single stranded DNA and is thought to contribute to apoptosis in the presence of bacterial and viral DNA. The expression of this gene is also found to be induced by tumor suppressor protein p53 in colon cancer cells.

Entrez Gene ID

Human	84883
Mouse	71361
Rat	361843
Monkey	711037
Hamster	100763473

UniProt ID

Human	Q9BRQ8
Mouse	Q8BUE4
Rat	D4AA14
Monkey	H2Q210
Hamster	A0A3L7IM23

Alternative Names

AMID; PRG3

Function

A NAD(P)H-dependent oxidoreductase involved in cellular oxidative stress response. At the plasma membrane, catalyzes reduction of coenzyme Q/ubiquinone-10 to ubiquinol-10, a lipophilic radical-trapping antioxidant that prevents lipid oxidative damage and consequently ferroptosis. Cooperates with GPX4 to suppress phospholipid peroxidation and ferroptosis. This anti-ferroptotic function is independent of cellular glutathione levels (PubMed:31634899, PubMed:31634900). May play a role in mitochondrial stress signaling. Upon oxidative stress, associates with the lipid peroxidation end product 4-hydroxy-2-nonenal (HNE) forming a lipid adduct devoid of oxidoreductase activity, which then translocates from mitochondria into the nucleus triggering DNA damage and cell death (PubMed:26689472). Capable of DNA binding in a non-sequence specific way (PubMed:15958387).

Biological Process

Apoptotic mitochondrial changes Source: MGI
Negative regulation of ferroptosis Source: UniProtKB
Positive regulation of apoptotic process Source: UniProtKB
Regulation of apoptotic process Source: Reactome
Regulation of cellular response to oxidative stress Source: UniProtKB
Ubiquinone metabolic process Source: UniProtKB

Cellular Location

Cytoplasm; Cell membrane; Nucleus; Mitochondrion membrane; Lipid droplet

Topology

Helical: 7-27 aa

PTM

N-myristoylation at Gly-2 mediates the recruitment to lipid droplets and plasma membrane.

Liu, L., Wang, M., Li, X., Wang, B., & Yin, S. (2021). Novel Role of IMPA2 in AIFM2-Mediated Apoptosis of Cervical Cancer by Targeting p53.

Tan, J. H., Cao, R. C., Zhou, L., Zhou, Z. T., Chen, H. J., Xu, J., ... & Zhang, G. W. (2020). ATF6 aggravates acinar cell apoptosis and injury by regulating p53/AIFM2 transcription in severe acute pancreatitis. Theranostics, 10(18), 8298.

Dai, E., Zhang, W., Cong, D., Kang, R., Wang, J., & Tang, D. (2020). AIFM2 blocks ferroptosis independent of ubiquinol metabolism. Biochemical and biophysical research communications, 523(4), 966-971.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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