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Mouse Anti-AIFM2 Recombinant Antibody (V2-356448) (CBMAB-P0032-YC)

Provided herein is a Mouse monoclonal antibody against Human Apoptosis inducing factor, mitochondria associated 2. The antibody can be used for immunoassay techniques, such as IP, FC, IF.
See all AIFM2 antibodies
Published Data
Mouse Anti-AIFM2 Recombinant Antibody (Pab122) in IHC
Phase-contrast microscope images of cell morphology of FSP1 DNA vector and insert transfected ARPE-19 cells. Scale bar = 100 µm. RFP red fluorescence protein, indicating successfully transfected cells. ...View More
Yang, M., Tsui, M. G., Tsang, J. K. W., Goit, R. K., Yao, K. M., So, K. F., ... & Lo, A. C. Y. (2022). Involvement of FSP1-CoQ10-NADH and GSH-GPx-4 pathways in retinal pigment epithelium ferroptosis. Cell death & disease, 13(5), 468.
Mouse Anti-AIFM2 Recombinant Antibody (Pab122) in WB
Western blot assay of the protein expression level of FSP1 in FSP1 DNA vector and insert transfected ARPE-19 cells. n = 3 (independent experiments). ...View More
Yang, M., Tsui, M. G., Tsang, J. K. W., Goit, R. K., Yao, K. M., So, K. F., ... & Lo, A. C. Y. (2022). Involvement of FSP1-CoQ10-NADH and GSH-GPx-4 pathways in retinal pigment epithelium ferroptosis. Cell death & disease, 13(5), 468.
Mouse Anti-AIFM2 Recombinant Antibody (Pab122) in WB
Western blot analyses and relative quantifications of FSP1 protein levels in a panel of melanoma cell lines, normalized to protein level in melanocytes (HEMa-LP). 1-way ANOVA with Dunnett's multiple comparisons test (HEMa-LP as a control) was used to quantify statistical significance. Error bars represent SEM, n ≥ 4. ...View More
Arslanbaeva, L., Tosi, G., Ravazzolo, M., Simonato, M., Tucci, F. A., Pece, S., ... & Santoro, M. M. (2022). UBIAD1 and CoQ10 protect melanoma cells from lipid peroxidation-mediated cell death. Redox Biology, 51, 102272.
Mouse Anti-AIFM2 Recombinant Antibody (Pab122) in WB
Western blot of GPD2, DHODH, FSP1, and GPX4 protein levels in HCT116 cells with the indicated genotypes. ...View More
Ma, P. W., Wang, W. L., Chen, J. W., Yuan, H., Lu, P. H., Gao, W., ... & Lu, L. J. (2022). Treatment with the ferroptosis inhibitor ferrostatin-1 attenuates noise-induced hearing loss by suppressing ferroptosis and apoptosis. Oxidative Medicine and Cellular Longevity, 2022.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
V2-356448
Antibody Isotype
IgG2a, κ
Application
IP, WB, IF, ELISA

Basic Information

Immunogen
Amino acids 1-300 mapping at the N-terminus of AMID of human origin.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:30-1:3,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Apoptosis Inducing Factor, Mitochondria Associated 2
Introduction
AIFM2 is a flavoprotein oxidoreductase that binds single stranded DNA and is thought to contribute to apoptosis in the presence of bacterial and viral DNA. The expression of this gene is also found to be induced by tumor suppressor protein p53 in colon cancer cells.
Entrez Gene ID
Human84883
Mouse71361
Rat361843
Monkey711037
Hamster100763473
UniProt ID
HumanQ9BRQ8
MouseQ8BUE4
RatD4AA14
MonkeyH2Q210
HamsterA0A3L7IM23
Alternative Names
AMID; PRG3
Function
A NAD(P)H-dependent oxidoreductase involved in cellular oxidative stress response. At the plasma membrane, catalyzes reduction of coenzyme Q/ubiquinone-10 to ubiquinol-10, a lipophilic radical-trapping antioxidant that prevents lipid oxidative damage and consequently ferroptosis. Cooperates with GPX4 to suppress phospholipid peroxidation and ferroptosis. This anti-ferroptotic function is independent of cellular glutathione levels (PubMed:31634899, PubMed:31634900). May play a role in mitochondrial stress signaling. Upon oxidative stress, associates with the lipid peroxidation end product 4-hydroxy-2-nonenal (HNE) forming a lipid adduct devoid of oxidoreductase activity, which then translocates from mitochondria into the nucleus triggering DNA damage and cell death (PubMed:26689472). Capable of DNA binding in a non-sequence specific way (PubMed:15958387).
Biological Process
Apoptotic mitochondrial changes Source: MGI
Negative regulation of ferroptosis Source: UniProtKB
Positive regulation of apoptotic process Source: UniProtKB
Regulation of apoptotic process Source: Reactome
Regulation of cellular response to oxidative stress Source: UniProtKB
Ubiquinone metabolic process Source: UniProtKB
Cellular Location
Cytoplasm; Cell membrane; Nucleus; Mitochondrion membrane; Lipid droplet
Topology
Helical: 7-27 aa
PTM
N-myristoylation at Gly-2 mediates the recruitment to lipid droplets and plasma membrane.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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