Mouse Anti-ALDH6A1 Recombinant Antibody (C9) (CBMAB-A2302-YC)

Name: Mouse Anti-ALDH6A1 Recombinant Antibody (C9)
SKU: CBMAB-A2302-YC
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-ALDH6A1 Recombinant Antibody (C9) in WB

Protein levels in individual HCC patients were determined by western blotting. ...View More

Shin, H., Cha, H. J., Lee, M. J., Na, K., Park, D., Kim, C. Y., ... & Paik, Y. K. (2020). Identification of ALDH6A1 as a potential molecular signature in hepatocellular carcinoma via quantitative profiling of the mitochondrial proteome. Journal of proteome research, 19(4), 1684-1695.

Mouse Anti-ALDH6A1 Recombinant Antibody (C9) in WB

Western blot analysis of six proteins differentially expressed in ACC and normal adrenal
Differential expression of ALDH6A1 (A), Transferrin (B), Fascin-1 (C), Lamin A/C (D), CAP-1 (E) and Ferredoxin reductase (FNR, F) as detected by a representative Western blot of the same pool of ACC and normal adrenal (NOR) samples used in 2D-DIGE. (G) Bar charts show mean±SE of relative expression levels for identified proteins vs actin, used as internal loading control and determined by densitometric analysis; n=3 independent Western blots. Statistical analysis with Student's t test: *p<0.01, ** p<0.005. ...View More

Poli, G., Ceni, E., Armignacco, R., Ercolino, T., Canu, L., Baroni, G., ... & Luconi, M. (2015). 2D-DIGE proteomic analysis identifies new potential therapeutic targets for adrenocortical carcinoma. Oncotarget, 6(8), 5695.

Mouse Anti-ALDH6A1 Recombinant Antibody (C9) in IHC

Immunohistochemical detection of the six proteins differentially expressed in ACC and normal adrenal
Representative images of immunohistochemistry performed on tumor specimens (n=4) and normal adrenals (n=3) revealed marked positivity to ALDH6A1 (A), Transferrin (B), Fascin-1 (C), CAP-1 (H) and Ferredoxin reductase (I) in the cytosol of almost all tumor cells in the field, compared to no positivity in normal adrenal, respectively (D-F, K-L). Nuclear positivity to Lamin A/C was diffused in tumor cells (G) and less expressed in normal adrenal cortex (J). ...View More

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

Application

ELISA, ICC, IF, IHC, WB

Immunogen

Amino acids 49-75 near the N-terminus of ALDH6A1 of human origin.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:30-1:3,000
IHC	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Aldehyde Dehydrogenase 6 Family Member A1

Introduction

ALDH6A1 is a member of the aldehyde dehydrogenase protein family. The encoded protein is a mitochondrial methylmalonate semialdehyde dehydrogenase that plays a role in the valine and pyrimidine catabolic pathways. This protein catalyzes the irreversible o

Entrez Gene ID

4329

UniProt ID

Q02252

Alternative Names

Aldehyde Dehydrogenase 6 Family Member A1; MMSDH; Methylmalonate-Semialdehyde Dehydrogenase [Acylating], Mitochondrial; Mitochondrial Acylating Methylmalonate-Semialdehyde Dehydrogenase; Malonate-Semialdehyde Dehydrogenase [Acylating]; Aldehyde Dehydrogen

Function

Plays a role in valine and pyrimidine metabolism. Binds fatty acyl-CoA.

Biological Process

Branched-chain amino acid catabolic process Source: Reactome
Brown fat cell differentiation Source: Ensembl
Thymine catabolic process Source: BHF-UCL
Thymine metabolic process Source: UniProtKB
Valine catabolic process Source: BHF-UCL
Valine metabolic process Source: UniProtKB

Cellular Location

Mitochondrion

Involvement in disease

Methylmalonate semialdehyde dehydrogenase deficiency (MMSDHD): A metabolic disorder characterized by elevated beta-alanine, 3-hydroxypropionic acid, and both isomers of 3-amino and 3-hydroxyisobutyric acids in urine organic acids.

Cho, S. Y., Kang, S., Kim, D. S., Na, H. J., Kim, Y. J., Choi, Y. D., & Cho, N. H. (2018). HSP27, ALDH6A1 and prohibitin act as a trio-biomarker to predict survival in late metastatic prostate cancer. Anticancer research, 38(11), 6551-6560.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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