Mouse Anti-ALPK2 Recombinant Antibody (4B5) (CBMAB-H0015-FY)

Name: Mouse Anti-ALPK2 Recombinant Antibody (4B5)
SKU: CBMAB-H0015-FY
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-ALPK2 Recombinant Antibody (4B5) in WB

The p.Q1853E variant of alpha-kinase 2 (ALPK2) reduces caspase-3 cleavage in HCT116 spheroids. A: Western blot analyses of ALPK2 expression in HKe3, parental (Par) HCT116 and HCT116 cells stably expressing wild-type (wt)ALPK2 (HCT116-wtALPK2) or amino acid-substituted (sub) ALPK2 (HCT116-subALPK2) grown in 2D culture. B: The area of spheroids formed by HCT116-derived cells at day 8 in 3D floating (3DF) culture. One thousand each of HCT116-wtALPK2 and -subALPK2 cells were seeded for 3DF culture. The areas of spheroids were measured at day 8. n.s.: Not significantly different. C: Expression of cleaved caspase-3 in HKe3, HCT116, HCT116-wtALPK2 and HCT116-subALPK2 cells at day 6 in a 3DF culture. β-Actin was used as a loading control. The upper panel shows western blotting results and the lower panel shows quantitative analyses of the relative expression levels. Bars represent the relative signal intensities of cells normalized to the signal of HCT116 cells. Significantly different at *p<0.05 and **p<0.005. ...View More

Nishi, K., Luo, H., Nakabayashi, K., Doi, K., Ishikura, S., Iwaihara, Y., ... & Tsunoda, T. (2017). An alpha-kinase 2 gene variant disrupts filamentous actin localization in the surface cells of colorectal cancer spheroids. Anticancer Research, 37(7), 3855-3862.

Mouse Anti-ALPK2 Recombinant Antibody (4B5) in IF

Signals for antibody to ALPK2 (green), phalloidin-labelled F-actin (red) and 4',6-diamidino-2-phenylindole (DAPI)-labelled nuclei (blue) in HKe3 cells grown in 2D culture. Scale bar=10 μm. ...View More

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

4B5

Application

ELISA, WB

Immunogen

ALPK2 (NP_443179, 201 a.a. ~ 309 a.a) partial recombinant protein with GST tag.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4

Preservative

None

Concentration

Batch dependent

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Alpha Kinase 2

Introduction

Kinase that recognizes phosphorylation sites in which the surrounding peptides have an alpha-helical conformation.

Entrez Gene ID

115701

UniProt ID

Q86TB3

Alternative Names

Alpha Kinase 2; Heart Alpha-Protein Kinase; Heart Alpha-Kinase; HAK; Alpha-Protein Kinase 2; EC 2.7.11.-

Function

Protein kinase that recognizes phosphorylation sites in which the surrounding peptides have an alpha-helical conformation (PubMed:10021370). Regulates cardiac development and cardiomyocyte differentiation by negatively regulating Wnt/beta-catenin signaling (PubMed:29888752).

Biological Process

Cardiac muscle cell development Source: UniProtKB
Epicardium morphogenesis Source: UniProtKB
Establishment of cell polarity Source: UniProtKB
Heart morphogenesis Source: UniProtKB
Negative regulation of Wnt signaling pathway involved in heart development Source: UniProtKB
Regulation of apoptotic process Source: UniProtKB
Regulation of gene expression Source: UniProtKB

Cellular Location

Basolateral cell membrane

Mamoor, S. (2021). ALPK2 is a differentially expressed gene in brain metastatic human breast cancer.

Wang, Y., Wu, J., Luo, W., Zhang, H., Shi, G., Shen, Y., ... & Zhu, Y. (2021). ALPK2 acts as tumor promotor in development of bladder cancer through targeting DEPDC1A. Cell death & disease, 12(7), 1-10.

McIntosh, A. T., Wei, R., Ahn, J., Aouizerat, B. E., Kassaye, S. G., Augenbraun, M. H., ... & Goldman, R. (2021). A genomic variant of ALPK2 is associated with increased liver fibrosis risk in HIV/HCV coinfected women. PloS one, 16(3), e0247277.

Bogomolovas, J., Feng, W., Yu, M. D., Huang, S., Zhang, L., Trexler, C., ... & Chen, J. (2020). Atypical ALPK2 kinase is not essential for cardiac development and function. American Journal of Physiology-Heart and Circulatory Physiology, 318(6), H1509-H1515.

Zhu, X., Yan, S., Xiao, S., & Xue, M. (2020). Knockdown of ALPK2 inhibits the development and progression of Ovarian Cancer. Cancer Cell International, 20(1), 1-10.

Jiang, J., Han, P., Qian, J., Zhang, S., Wang, S., Cao, Q., & Shao, P. (2020). Knockdown of ALPK2 blocks development and progression of renal cell carcinoma. Experimental cell research, 392(2), 112029.

Hofsteen, P., Robitaille, A. M., Strash, N., Palpant, N., Moon, R. T., Pabon, L., & Murry, C. E. (2018). ALPK2 promotes cardiogenesis in zebrafish and human pluripotent stem cells. Iscience, 2, 88-100.

Nishi, K., Luo, H., Nakabayashi, K., Doi, K., Ishikura, S., Iwaihara, Y., ... & Tsunoda, T. (2017). An Alpha-kinase 2 gene variant disrupts filamentous actin localization in the surface cells of colorectal cancer spheroids. Anticancer research, 37(7), 3855-3862.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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