Mouse Anti-AMIGO1 Recombinant Antibody (S86-36) (CBMAB-A2539-YC)

Name: Mouse Anti-AMIGO1 Recombinant Antibody (S86-36)
SKU: CBMAB-A2539-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

S86-36

Application

ICC, IHC, WB

Immunogen

Fusion protein amino acids 394-492 (cytoplasmic C-terminus) of mouse AMIGO-1.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH7.4, 50% glycerol

Preservative

0.09% sodium azide

Concentration

1 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

aa 554-574

More Infomation

Target

Full Name

Adhesion Molecule With Ig Like Domain 1

Introduction

AMIGO1 promotes growth and fasciculation of neurites from cultured hippocampal neurons. AMIGO1 may be involved in fasciculation as well as myelination of developing neural axons. AMIGO1 may have a role in regeneration as well as neural plasticity in the a

Entrez Gene ID

57463

UniProt ID

Q86WK6

Alternative Names

Adhesion Molecule With Ig Like Domain 1; Alivin-2; AMIGO-1; AMIGO; ALI2; Amphoterin-Induced Gene And Open Reading Frame; Amphoterin-Induced Gene And ORF; Amphoterin-Induced Protein 1; KIAA1163;

Function

Promotes growth and fasciculation of neurites from cultured hippocampal neurons. May be involved in fasciculation as well as myelination of developing neural axons. May have a role in regeneration as well as neural plasticity in the adult nervous system. May mediate homophilic as well as heterophilic cell-cell interaction and contribute to signal transduction through its intracellular domain. Assembled with KCNB1 modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1.

Biological Process

Axonal fasciculation Source: UniProtKB
Axonogenesis Source: Ensembl
Brain development Source: GO_Central
Cellular response to L-glutamate Source: Ensembl
Heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules Source: UniProtKB
Homophilic cell adhesion via plasma membrane adhesion molecules Source: UniProtKB
Myelination Source: UniProtKB
Positive regulation of axonogenesis Source: UniProtKB
Positive regulation of neuron projection development Source: Ensembl
Positive regulation of potassium ion transmembrane transport Source: UniProtKB
Positive regulation of synapse assembly Source: Ensembl
Positive regulation of voltage-gated potassium channel activity Source: Ensembl

Cellular Location

Cell membrane; Perikaryon; Dendrite; Axon. Colocalizes with KCNB1 at high-density somatodendritic clusters on the surface of hippocampal and cortical neurons. Associated with axons of neuronal cells.

Topology

Extracellular: 28-372 aa
Helical: 373-393 aa
Cytoplasmic: 394-493 aa

Maverick, E. E., Leek, A. N., & Tamkun, M. M. (2021). Kv2 channel–AMIGO β-subunit assembly modulates both channel function and cell adhesion molecule surface trafficking. Journal of cell science, 134(12), jcs256339.

Bishop, H. I., Cobb, M. M., Kirmiz, M., Parajuli, L. K., Mandikian, D., Philp, A. M., ... & Trimmer, J. S. (2018). Kv2 ion channels determine the expression and localization of the associated AMIGO-1 cell adhesion molecule in adult brain neurons. Frontiers in Molecular Neuroscience, 11, 1.

Nagatti, D. (2017). Analyzing Intracellular Short Linear Motifs of AMIGO and NGL Orthologs.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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