Mouse Anti-AMT Recombinant Antibody (4C9) (CBMAB-A2590-YC)

Provided herein is a Mouse monoclonal antibody against Human Aminomethyltransferase. The antibody can be used for immunoassay techniques, such as WB.
See all AMT antibodies

Summary

Host Animal

Mouse

Specificity

Human, Mouse, Rat

Clone

4C9

Antibody Isotype

IgG1

Application

Basic Information

Immunogen

Human recombinant protein fragment corresponding to amino acids 146-403 of human AMT(NP_000472) produced in E. coli

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:2,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.3, 1% BSA, 50% glycerol

Preservative

0.02% sodium azide

Concentration

1 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

Aminomethyltransferase

Introduction

AMT is one of four critical components of the glycine cleavage system. Mutations in this gene have been associated with glycine encephalopathy.

Entrez Gene ID

275

UniProt ID

P48728

Alternative Names

Aminomethyltransferase; Glycine Cleavage System T Protein; EC 2.1.2.10; GCST; GCVT; Aminomethyltransferase (Glycine Cleavage System Protein T);

Function

The glycine cleavage system catalyzes the degradation of glycine.

Biological Process

Glycine catabolic process Source: Reactome
Glycine decarboxylation via glycine cleavage system Source: UniProtKB

Cellular Location

Mitochondrion

Involvement in disease

Non-ketotic hyperglycinemia (NKH): Autosomal recessive disease characterized by accumulation of a large amount of glycine in body fluid and by severe neurological symptoms.

References

Simmons, R. M., McKnight, S. M., Edwards, A. K., Wu, G., & Satterfield, M. C. (2020). Obesity increases hepatic glycine dehydrogenase and aminomethyltransferase expression while dietary glycine supplementation reduces white adipose tissue in Zucker diabetic fatty rats. Amino Acids, 52(10), 1413-1423.

Silverstein, S., Veerapandiyan, A., Hayes-Rosen, C., Ming, X., & Kornitzer, J. (2019). A novel intronic homozygous mutation in the AMT gene of a patient with nonketotic hyperglycinemia and hyperammonemia. Metabolic brain disease, 34(1), 373-376.

Devi, A. R. R., Lingappa, L., & Naushad, S. M. (2018). Identification of Two Novel Mutations in Aminomethyltransferase Gene in Cases of Glycine Encephalopathy. Journal of pediatric genetics, 7(03), 097-102.

Kornitzer, J., Veerapandiyan, A., Jakubowski, L., Hayes-Rosen, C., & Ming, X. (2017). Homozygous AMT mutation in a patient with neonatal onset nonketotic hyperglycinemia and hyperammonemia (P4. 178).

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Related Products

Mouse Anti-AMT Recombinant Antibody (6F4)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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