Mouse Anti-ANAPC10 Recombinant Antibody (CBT4225) (V2LY-0625-LY666)

Name: Mouse Anti-ANAPC10 Recombinant Antibody (CBT4225)
SKU: V2LY-0625-LY666
Rating: 5 (1 reviews)

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Tested Data

Mouse Anti-ANAPC10 Recombinant Antibody (CBT4225) (V2LY-0625-LY666) in ELISA

Mouse Anti-ANAPC10 Recombinant Antibody (CBT4225) (V2LY-0625-LY666) in WB

Mouse Anti-ANAPC10 Recombinant Antibody (CBT4225) (V2LY-0625-LY666) in IF

Mouse Anti-ANAPC10 Recombinant Antibody (CBT4225) (V2LY-0625-LY666) in IHC

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBT4225

Application

WB, IHC, ICC

Immunogen

Purified recombinant fragment of human ANAPC10 (AA: 1-185) expressed in E. Coli.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
IHC-P	1:200-1:1,000
ICC	1:200-1:1,000
ELISA	1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Anaphase Promoting Complex Subunit 10

Entrez Gene ID

10393

UniProt ID

Q9UM13

Function

Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.

Biological Process

Anaphase-promoting complex-dependent catabolic process Source: Reactome
Cell cycle Source: UniProtKB-KW
Cell division Source: UniProtKB-KW
Protein K11-linked ubiquitination Source: UniProtKB
Regulation of exit from mitosis Source: Reactome
Regulation of mitotic cell cycle phase transition Source: Reactome
Ubiquitin-dependent protein catabolic process Source: Reactome

Cellular Location

Cytosol; Anaphase-promoting complex; Nucleoplasm

Huang, S., Wan, P., Huang, S., Xiang, Q., Yang, G., Shereen, M., ... & Wu, J. (2021). Anapc10 orchestrates the NLRP3 inflammasome activation during cell cycle control.

Saito, K., Michon, F., Yamada, A., Inuzuka, H., Yamaguchi, S., Fukumoto, E., ... & Fukumoto, S. (2020). Sox21 regulates Anapc10 expression and determines the fate of ectodermal organ. Iscience, 23(7), 101329.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-ANAPC10 Recombinant Antibody (CBT4292) (CAT#: V2LY-0625-LY665)

Human Recombinant ANAPC10 protein, His Tag (CAT#: V2LY-0526-LY2133)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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