Human Recombinant ANAPC10 protein, His Tag (V2LY-0526-LY2133)

Name: Human Recombinant ANAPC10 protein, His Tag
SKU: V2LY-0526-LY2133
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant ANAPC10 protein, His Tag consist of Amino Acid: 1-185 and predicts a molecular mass of 24 kDa.

Molecule Mass

24 kDa

Sequence

Amino Acid: 1-185

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

Batch dependent.

Endotoxin

Please contact us for more information.

Format

Liquid

Buffer

Tris, NaCl, Brij-35

Preservative

None

Storage

Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

More Infomation

Target

Full Name

Anaphase Promoting Complex Subunit 10

Function

Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.

Biological Process

Anaphase-promoting complex-dependent catabolic process Source: Reactome
Cell cycle Source: UniProtKB-KW
Cell division Source: UniProtKB-KW
Protein K11-linked ubiquitination Source: UniProtKB
Regulation of exit from mitosis Source: Reactome
Regulation of mitotic cell cycle phase transition Source: Reactome
Ubiquitin-dependent protein catabolic process Source: Reactome

Cellular Location

Cytosol; Anaphase-promoting complex; Nucleoplasm

Huang, S., Wan, P., Huang, S., Xiang, Q., Yang, G., Shereen, M., ... & Wu, J. (2021). Anapc10 orchestrates the NLRP3 inflammasome activation during cell cycle control.

Saito, K., Michon, F., Yamada, A., Inuzuka, H., Yamaguchi, S., Fukumoto, E., ... & Fukumoto, S. (2020). Sox21 regulates Anapc10 expression and determines the fate of ectodermal organ. Iscience, 23(7), 101329.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Mouse Anti-ANAPC10 Recombinant Antibody (CBT4292) (CAT#: V2LY-0625-LY665)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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