Mouse Anti-ANG Recombinant Antibody (CAP131) (CBMAB-AP311LY)

Name: Mouse Anti-ANG Recombinant Antibody (CAP131)
SKU: CBMAB-AP311LY
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CAP131

Application

ELISA IHC, IP, WB

Immunogen

ANGPT4 (NP_057069, 25 a.a. ~ 111 a.a) partial recombinant protein with GST tag.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
IHC-P	3 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4

Preservative

None

Concentration

Batch dependent

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Angiogenin

Introduction

The protein encoded by this gene is an exceedingly potent mediator of new blood vessel formation. It hydrolyzes cellular tRNAs resulting in decreased protein synthesis and is similar to pancreatic ribonuclease. In addition, the mature peptide has antimicrobial activity against some bacteria and fungi, including S. pneumoniae and C. albicans. Alternative splicing results in two transcript variants encoding the same protein. This gene and the gene that encodes ribonuclease, RNase A family, 4 share promoters and 5' exons. Each gene splices to a unique downstream exon that contains its complete coding region. [provided by RefSeq, Aug 2014]

Entrez Gene ID

777597

UniProt ID

P10152

Alternative Names

Angiogenin; Angiogenin, Ribonuclease, RNase A Family, 5; Ribonuclease A Family Member 5; Ribonuclease 5; RNase 5; RNASE5; Epididymis Luminal Protein 168; Ribonuclease A A1;

Function

Binds to actin on the surface of endothelial cells; once bound, angiogenin is endocytosed and translocated to the nucleus. Stimulates ribosomal RNA synthesis including that containing the initiation site sequences of 45S rRNA. Cleaves tRNA within anticodon loops to produce tRNA-derived stress-induced fragments (tiRNAs) which inhibit protein synthesis and triggers the assembly of stress granules (SGs). Angiogenin induces vascularization of normal and malignant tissues. Angiogenic activity is regulated by interaction with RNH1 in vivo.

Biological Process

Actin filament polymerization Source: UniProtKB
Activation of phospholipase A2 activity Source: UniProtKB
Activation of phospholipase C activity Source: UniProtKB
Activation of protein kinase B activity Source: UniProtKB
Adherens junction organization Source: Reactome
Angiogenesis Source: MGI
Antibacterial humoral response Source: UniProtKB
Antifungal humoral response Source: UniProtKB
Antimicrobial humoral immune response mediated by antimicrobial peptide Source: UniProtKB
Cell communication Source: UniProtKB
Cell migration Source: UniProtKB
Defense response to Gram-positive bacterium Source: UniProtKB
Diacylglycerol biosynthetic process Source: UniProtKB
Homeostatic process Source: UniProtKB
Innate immune response Source: UniProtKB
Negative regulation of smooth muscle cell proliferation Source: UniProtKB
Negative regulation of translation Source: UniProtKB-KW
Oocyte maturation Source: UniProtKB
Ovarian follicle development Source: UniProtKB
Placenta development Source: UniProtKB
Positive regulation of endothelial cell proliferation Source: UniProtKB
Positive regulation of phosphorylation Source: UniProtKB
Positive regulation of protein secretion Source: UniProtKB
Response to hormone Source: UniProtKB
Response to hypoxia Source: UniProtKB
Response to yeast Source: UniProtKB
rRNA transcription Source: UniProtKB

Cellular Location

Nucleus; Nucleolus; Secreted; Secretory vesicle lumen. Rapidly endocytosed by target cells and translocated to the nucleus where it accumulates in the nucleolus and binds to DNA (PubMed:12051708).

Involvement in disease

Amyotrophic lateral sclerosis 9 (ALS9): A neurodegenerative disorder affecting upper motor neurons in the brain and lower motor neurons in the brain stem and spinal cord, resulting in fatal paralysis. Sensory abnormalities are absent. The pathologic hallmarks of the disease include pallor of the corticospinal tract due to loss of motor neurons, presence of ubiquitin-positive inclusions within surviving motor neurons, and deposition of pathologic aggregates. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of the cases.

Aluri, K. C., Salisbury, J. P., Prehn, J. H., & Agar, J. N. (2020). Loss of angiogenin function is related to earlier ALS onset and a paradoxical increase in ALS duration. Scientific reports, 10(1), 1-13.

Su, Z., Kuscu, C., Malik, A., Shibata, E., & Dutta, A. (2019). Angiogenin generates specific stress-induced tRNA halves and is not involved in tRF-3–mediated gene silencing. Journal of Biological Chemistry, 294(45), 16930-16941.

Li, S., Shi, X., Chen, M., Xu, N., Sun, D., Bai, R., ... & Xu, Z. (2019). Angiogenin promotes colorectal cancer metastasis via tiRNA production. International journal of cancer, 145(5), 1395-1407.

Thomas, S. P., Hoang, T. T., Ressler, V. T., & Raines, R. T. (2018). Human angiogenin is a potent cytotoxin in the absence of ribonuclease inhibitor. Rna, 24(8), 1018-1027.

Yu, D., Cai, Y., Zhou, W., Sheng, J., & Xu, Z. (2018). The potential of angiogenin as a serum biomarker for diseases: systematic review and meta-analysis. Disease markers, 2018.

Zhang, Y., Xia, X., Yan, J., Yan, L., Lu, C., Zhu, X., ... & Qiao, J. (2017). Mesenchymal stem cell-derived angiogenin promotes primodial follicle survival and angiogenesis in transplanted human ovarian tissue. Reproductive Biology and Endocrinology, 15(1), 1-12.

Yu, W., Goncalves, K. A., Li, S., Kishikawa, H., Sun, G., Yang, H., ... & Hu, G. F. (2017). Plexin-B2 mediates physiologic and pathologic functions of angiogenin. Cell, 171(4), 849-864.

Lyons, S. M., Fay, M. M., Akiyama, Y., Anderson, P. J., & Ivanov, P. (2017). RNA biology of angiogenin: Current state and perspectives. RNA biology, 14(2), 171-178.

Mo, Y., Guo, R., Zhang, Y., Xue, W., Cheng, B., & Zhang, Y. (2017). Controlled dual delivery of angiogenin and curcumin by electrospun nanofibers for skin regeneration. Tissue Engineering Part A, 23(13-14), 597-608.

Hoang, T. T., & Raines, R. T. (2017). Molecular basis for the autonomous promotion of cell proliferation by angiogenin. Nucleic acids research, 45(2), 818-831.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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