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Mouse Anti-ANGPT2 Recombinant Antibody (CBYC-A587) (CBMAB-A2681-YC)

Provided herein is a Mouse monoclonal antibody against Human Angiopoietin 2. The antibody can be used for immunoassay techniques, such as ELISA, FuncS.
See all ANGPT2 antibodies
Published Data
Mouse Anti-ANGPT2 Recombinant Antibody (CBYC-A587) in WB
Effect of TSLP (10 µg) on VEGFA and Ang-2 expression in the infarct area of rats following MCAO. ...View More
Yu, X., Peng, Y., Liang, H., Fu, K., Zhao, Z., Xie, C., ... & Zhang, K. (2018). TSLP/TSLPR promote angiogenesis following ischemic stroke via activation of the PI3K/AKT pathway. Molecular medicine reports, 17(2), 3411-3417.
Mouse Anti-ANGPT2 Recombinant Antibody (CBYC-A587) in IHC
Immunohistochemical and quantification analysis of ANG2 protein expression in normal liver tissue (up) and OSCC (bottom) samples using specific anti-ANG2 antibody. Representative photographs were obtained at × 200 magnification. ...View More
Li, C., Li, Q., Cai, Y., He, Y., Lan, X., Wang, W., ... & Sun, R. (2016). Overexpression of angiopoietin 2 promotes the formation of oral squamous cell carcinoma by increasing epithelial-mesenchymal transition-induced angiogenesis. Cancer gene therapy, 23(9), 295-302.
Mouse Anti-ANGPT2 Recombinant Antibody (CBYC-A587) in WB
Western blot analysis of the three peptides' effects on renin, ACE, Ang II, ACE2, and Ang (1-7) in myocardium tissue of spontaneously hypertensive rats (SHRs) compared with that of WKY rats. GAPDH was used as internal control. ...View More
He, R., Yang, Y. J., Wang, Z., Xing, C. R., Yuan, J., Wang, L. F., ... & Ju, X. R. (2019). Rapeseed protein-derived peptides, LY, RALP, and GHS, modulates key enzymes and intermediate products of renin-angiotensin system pathway in spontaneously hypertensive rat. NPJ science of food, 3(1), 1.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBYC-A587
Antibody Isotype
IgG1, κ
Application
WB, IHC, ICC, IF

Basic Information

Immunogen
Amino acids 171-240 mapping within an internal region of Ang-2 of Ang-2 of human origin.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:30-1:3,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Angiopoietin 2
Introduction
ANGPT2 is an antagonist of angiopoietin 1 (ANGPT1) and endothelial TEK tyrosine kinase (TIE-2, TEK). The encoded protein disrupts the vascular remodeling ability of ANGPT1 and may induce endothelial cell apoptosis.
Entrez Gene ID
Human285
Mouse11601
UniProt ID
HumanO15123
MouseO35608
Alternative Names
Angiopoietin 2; ANG-2; Angiopoietin-2B; Angiopoietin-2a; Angiopoietin-2; Tie2-Ligand; AGPT2; ANG2;
Function
Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal.
Biological Process
Angiogenesis Source: GO_Central
Animal organ regeneration Source: Ensembl
Cellular response to growth factor stimulus Source: Ensembl
Gene expression Source: MGI
Germ cell development Source: Ensembl
Glomerulus vasculature development Source: UniProtKB
Leukocyte migration Source: Reactome
Maternal process involved in female pregnancy Source: Ensembl
Negative regulation of angiogenesis Source: Ensembl
Negative regulation of blood vessel endothelial cell migration Source: UniProtKB
Negative regulation of cell-substrate adhesion Source: Ensembl
Negative regulation of positive chemotaxis Source: UniProtKB
Positive regulation of angiogenesis Source: Ensembl
Response to activity Source: Ensembl
Response to glucose Source: Ensembl
Response to hypoxia Source: Ensembl
Response to mechanical stimulus Source: Ensembl
Response to organic cyclic compound Source: Ensembl
Response to radiation Source: Ensembl
Signal transduction Source: ProtInc
Tie signaling pathway Source: UniProtKB
Cellular Location
Secreted

Kamiyama, M., & Augustin, H. G. (2021). Alternatively Spliced Form of Angiopoietin-2 as a New Vascular Rheostat. Cancer Research, 81(1), 35-37.

Aarsetøy, R., Ueland, T., Aukrust, P., Michelsen, A. E., de la Fuente, R. L., Pönitz, V., ... & Nilsen, D. W. (2021). Angiopoietin‐2 and angiopoietin‐like 4 protein provide prognostic information in patients with suspected acute coronary syndrome. Journal of internal medicine.

Hultstrom, M., Fromell, K., Larsson, A., Quaggin, S. E., Betsholtz, C., Frithiof, R., ... & Jeansson, M. (2021). Elevated Angiopoietin-2 inhibits thrombomodulin-mediated anticoagulation in critically ill COVID-19 patients. medRxiv.

Xie, J. Y., Wei, J. X., Lv, L. H., Han, Q. F., Yang, W. B., Li, G. L., ... & Min, J. (2020). Angiopoietin-2 induces angiogenesis via exosomes in human hepatocellular carcinoma. Cell Communication and Signaling, 18(1), 1-13.

Pari, A. A. A., Singhal, M., Hübers, C., Mogler, C., Schieb, B., Gampp, A., ... & Felcht, M. (2020). Tumor Cell–Derived Angiopoietin-2 Promotes Metastasis in Melanoma. Cancer research, 80(12), 2586-2598.

Crist, A. M., Zhou, X., Garai, J., Lee, A. R., Thoele, J., Ullmer, C., ... & Meadows, S. M. (2019). Angiopoietin-2 inhibition rescues arteriovenous malformation in a Smad4 hereditary hemorrhagic telangiectasia mouse model. Circulation, 139(17), 2049-2063.

Schlosser, K., Taha, M., Deng, Y., McIntyre, L. A., Mei, S. H., & Stewart, D. J. (2018). High circulating angiopoietin-2 levels exacerbate pulmonary inflammation but not vascular leak or mortality in endotoxin-induced lung injury in mice. Thorax, 73(3), 248-261.

Souma, T., Thomson, B. R., Heinen, S., Carota, I. A., Yamaguchi, S., Onay, T., ... & Quaggin, S. E. (2018). Context-dependent functions of angiopoietin 2 are determined by the endothelial phosphatase VEPTP. Proceedings of the National Academy of Sciences, 115(6), 1298-1303.

Reilly, J. P., Wang, F., Jones, T. K., Palakshappa, J. A., Anderson, B. J., Shashaty, M. G., ... & Meyer, N. J. (2018). Plasma angiopoietin-2 as a potential causal marker in sepsis-associated ARDS development: evidence from Mendelian randomization and mediation analysis. Intensive care medicine, 44(11), 1849-1858.

Wu, X., Giobbie-Hurder, A., Liao, X., Connelly, C., Connolly, E. M., Li, J., ... & Hodi, F. S. (2017). Angiopoietin-2 as a biomarker and target for immune checkpoint therapy. Cancer immunology research, 5(1), 17-28.

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For research use only. Not intended for any clinical use.

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