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Mouse Anti-ANKS1B Recombinant Antibody (CBYC-A613) (CBMAB-A2806-YC)

Provided herein is a Mouse monoclonal antibody against Human Ankyrin Repeat And Sterile Alpha Motif Domain Containing 1B. The antibody can be used for immunoassay techniques, such as WB, IHC-P, ELISA.
See all ANKS1B antibodies
Published Data
Mouse Anti-ANKS1B Recombinant Antibody (CBYC-A613) in WB
Western blots showing the approximately 50 and 57 kD isoforms of Anks1b (AIDA-1e and AIDA-1d respectively) in mice of different genotypes for the tm1a and tm1b alleles. ...View More
Enga, R. M., Rice, A. C., Weller, P., Subler, M. A., Lee, D., Hall, C. P., ... & McClay, J. L. (2017). Initial characterization of behavior and ketamine response in a mouse knockout of the post-synaptic effector gene Anks1b. Neuroscience letters, 641, 26-32.
Mouse Anti-ANKS1B Recombinant Antibody (CBYC-A613) in IP
Western blots showing AIDA-1 isoforms immunoprecipitated by AIDA-1 antibody combinations. Only antibody 1A11 (included in AIDA-1 Mix) was able to pull down AIDA-1B. ...View More
Carbonell, A. U., Cho, C. H., Tindi, J. O., Counts, P. A., Bates, J. C., Erdjument-Bromage, H., ... & Jordan, B. A. (2019). Haploinsufficiency in the ANKS1B gene encoding AIDA-1 leads to a neurodevelopmental syndrome. Nature communications, 10(1), 3529.

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBYC-A613
Antibody Isotype
IgG1, κ
Application
WB, IP, IF, ELISA

Basic Information

Immunogen
Amino acids 847-1054 mapping near the C-terminus of AIDA-1 of human origin.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:30-1:3,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Ankyrin Repeat And Sterile Alpha Motif Domain Containing 1B
Introduction
ANKS1B is a multi-domain protein that is predominantly expressed in brain and testis. This protein interacts with amyloid beta protein precursor (AbetaPP) and may have a role in normal brain development, and in the pathogenesis of Alzheimer's disease. Exp
Entrez Gene ID
Human56899
Rat314721
UniProt ID
HumanQ7Z6G8
RatP0C6S7
Alternative Names
Ankyrin Repeat And Sterile Alpha Motif Domain Containing 1B; AIDA-1; EB-1; Amyloid-Beta Precursor Protein Intracellular Domain Associated Protein 1; Ankyrin Repeat And Sterile Alpha Motif Domain-Containing Protein 1B; Amyloid-Beta Protein Intracellular Do
Function
Isoform 2 may participate in the regulation of nucleoplasmic coilin protein interactions in neuronal and transformed cells.
Isoform 3 can regulate global protein synthesis by altering nucleolar numbers.
Isoform 4 may play a role as a modulator of APP processing. Overexpression can down-regulate APP processing.
Biological Process
Ephrin receptor signaling pathway Source: GO_Central
Regulation of synaptic plasticity by receptor localization to synapse Source: InterPro
Cellular Location
Cytoplasm
Isoform 2: Nucleus
Isoform 3: Nucleus; Cajal body; Postsynaptic density; Dendritic spine. The synaptic localization requires DLG4 interaction. Translocation to the nucleus in response to stimulation of NMDA receptors (NMDARs) in a calcium-independent manner (By similarity).
Isoform 4: Nucleus. The interaction with APP causes its partial exclusion from the nucleus, when APP is overexpressed.
Isoform 6: Nucleus
PTM
Isoform 3 nuclear translocation requires an NMDAR-dependent proteolytic cleavage.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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