Rat Anti-ANKS1B Recombinant Antibody (KT51) (CBMAB-A2805-YC)

Name: Rat Anti-ANKS1B Recombinant Antibody (KT51)
SKU: CBMAB-A2805-YC
Rating: 5 (1 reviews)

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Published Data

Rat Anti-ANKS1B Recombinant Antibody (KT51) in IP

Test native EB1 protein in immunoprecipitates with GFP rabbit Ab (left) and dsR rabbit Ab (right). WCL and three lanes for each IP reaction (Super = WCL after IP, [-] IP without Ab, [+] IP with Ab) were fractionated by SDS-PAGE. After proteins were transferred to PVDF membrane, the membrane was cut below 75 kDa size marker band. The upper portion was probed with GFP goat Ab. The lower portion was first probed with HA mouse Ab, and after stripping, reprobed with EB1 rat Ab. ...View More

Wilson, Z. T., Jiang, M., Geng, J., Kaur, S., Workman, S. W., Hao, J., ... & Tseng, G. N. (2021). Delayed KCNQ1/KCNE1 assembly on the cell surface helps IKs fulfil its function as a repolarization reserve in the heart. The Journal of physiology, 599(13), 3337-3361.

Rat Anti-ANKS1B Recombinant Antibody (KT51) in IF

Immunofluorescence/confocal microscopy: Fixed myocytes without membrane permeabilization were incubated with Q1 ext rabbit Ab to label cell surface Q1. After membrane permeabilization, myocytes were incubated with GFP mouse Ab (binding total pool of Q1-GFP) and EB1 rat Ab (binding native EB1). ...View More

Rat Anti-ANKS1B Recombinant Antibody (KT51) in IP

Representative Western blot analysis of the endogenous p150Glued co-immunoprecipitated with EB1 in shCTL or shSTIM1 ECs (left) and its quantification by normalized densitometry (right). Negative control (CTL) was performed incubating cell lysate with protein A- or G-Sepharose and empty mouse IgG. Results are the average ± SD of three independent assays. shCTL value of each biological replicate was normalized on itself and so shSTIM1 experimental value. Results were analyzed by a parametric two-tailed analysis of variance (ANOVA) with Bonferroni post hoc analysis. ANOVA P ≤ 0.001; Bonferroni for shCTL and shSTIM1 P ≤ 0.05*. ...View More

Villari, G., Enrico Bena, C., Del Giudice, M., Gioelli, N., Sandri, C., Camillo, C., ... & Serini, G. (2020). Distinct retrograde microtubule motor sets drive early and late endosome transport. The EMBO journal, 39(24), e103661.

Datasheet

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rat

Clone

KT51

Application

ELISA, WB, IP, IF

Immunogen

Recombinant human EB1(aa 125-268)-GST fusion protein

Specificity

Human, Mouse, Monkey

Antibody Isotype

IgG2a

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
FC	1 µg/10^6 cells

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Ankyrin Repeat And Sterile Alpha Motif Domain Containing 1B

Introduction

ANKS1B is a multi-domain protein that is predominantly expressed in brain and testis. This protein interacts with amyloid beta protein precursor (AbetaPP) and may have a role in normal brain development, and in the pathogenesis of Alzheimer's disease. Exp

Entrez Gene ID

Human	56899
Mouse	77531
Monkey	694033

UniProt ID

Human	Q7Z6G8
Mouse	Q8BIZ1
Monkey	A0A2K6ULF1

Alternative Names

Ankyrin Repeat And Sterile Alpha Motif Domain Containing 1B; AIDA-1; EB-1; Amyloid-Beta Precursor Protein Intracellular Domain Associated Protein 1; Ankyrin Repeat And Sterile Alpha Motif Domain-Containing Protein 1B; Amyloid-Beta Protein Intracellular Do

Function

Isoform 2 may participate in the regulation of nucleoplasmic coilin protein interactions in neuronal and transformed cells.
Isoform 3 can regulate global protein synthesis by altering nucleolar numbers.
Isoform 4 may play a role as a modulator of APP processing. Overexpression can down-regulate APP processing.

Biological Process

Ephrin receptor signaling pathway Source: GO_Central
Regulation of synaptic plasticity by receptor localization to synapse Source: InterPro

Cellular Location

Cytoplasm
Isoform 2: Nucleus
Isoform 3: Nucleus; Cajal body; Postsynaptic density; Dendritic spine. The synaptic localization requires DLG4 interaction. Translocation to the nucleus in response to stimulation of NMDA receptors (NMDARs) in a calcium-independent manner (By similarity).
Isoform 4: Nucleus. The interaction with APP causes its partial exclusion from the nucleus, when APP is overexpressed.
Isoform 6: Nucleus

PTM

Isoform 3 nuclear translocation requires an NMDAR-dependent proteolytic cleavage.

Younis, R. M., Taylor, R. M., Beardsley, P. M., & McClay, J. L. (2019). The ANKS1B gene and its associated phenotypes: focus on CNS drug response. Pharmacogenomics, 20(9), 669-684.

Enga, R. M., Rice, A. C., Weller, P., Subler, M. A., Lee, D., Hall, C. P., ... & McClay, J. L. (2017). Initial characterization of behavior and ketamine response in a mouse knockout of the post-synaptic effector gene Anks1b. Neuroscience letters, 641, 26-32.

Kang, S. G., Chee, I. S., Lee, K., & Lee, J. (2017). rs7968606 polymorphism of ANKS1B is associated with improvement in the PANSS general score of schizophrenia caused by amisulpride. Human Psychopharmacology: Clinical and Experimental, 32(2), e2562.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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