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Mouse Anti-ANO1 Recombinant Antibody (CBYJT-1038) (CBMAB-T0042-YJ)

Provided herein is a Mouse monoclonal antibody, which binds to ANO1 (Anoctamin 1). The antibody can be used for immunoassay techniques, such as WB, IP, IHC-P, IHC-Fr, FC, ICC, IF.
See all ANO1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBYJT-1038
Antibody Isotype
IgG2b, κ
Application
IHC, MA

Basic Information

Immunogen
Recombinant human canine1/TMEM16A protein fragment (around aa 2-101).
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
IHC-P1-2 μg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.05% BSA
Preservative
0.05% sodium azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
aa 1-986

Target

Full Name
Anoctamin 1
Introduction
ANO1 is a calcium-activated chloride channel (CaCC) which functions in transepithelial anion transport and smooth muscle contraction. It is required for the normal functioning of the interstitial cells of Cajal (ICCs) which generate electrical pacemaker activity in gastrointestinal smooth muscles. ANO1 acts as a major contributor to basal and stimulated chloride conductance in airway epithelial cells and plays an important role in tracheal cartilage development.
Entrez Gene ID
55107
UniProt ID
Q5XXA6
Alternative Names
Anoctamin 1; Discovered On Gastrointestinal Stromal Tumors Protein 1; Anoctamin 1, Calcium Activated Chloride Channel; Tumor-Amplified And Overexpressed Sequence 2; Oral Cancer Overexpressed 2; Transmembrane Protein 16A; TMEM16A; ORAOV2
Function
Calcium-activated chloride channel (CaCC) which plays a role in transepithelial anion transport and smooth muscle contraction. Required for the normal functioning of the interstitial cells of Cajal (ICCs) which generate electrical pacemaker activity in gastrointestinal smooth muscles. Acts as a major contributor to basal and stimulated chloride conductance in airway epithelial cells and plays an important role in tracheal cartilage development.
Biological Process
Cation transport Source: UniProtKB
Cellular response to heat Source: UniProtKB
Cellular response to peptide Source: Ensembl
Chloride transmembrane transport Source: UniProtKB
Chloride transport Source: UniProtKB
Detection of temperature stimulus involved in sensory perception of pain Source: UniProtKB
Iodide transport Source: UniProtKB
Ion transmembrane transport Source: Reactome
Multicellular organism development Source: UniProtKB-KW
Phospholipase C-activating G protein-coupled receptor signaling pathway Source: UniProtKB
Positive regulation of insulin secretion involved in cellular response to glucose stimulus Source: UniProtKB
Transmembrane transport Source: GO_Central
Cellular Location
Cytoplasm; Cell membrane. Cytoplasmic localization seen in neoplastic cells of head and neck squamous cell carcinoma (HNSCC) tumors.
Topology
Cytoplasmic: 1-333 aa
Helical: 334-354 aa
Extracellular: 355-406 aa
Helical: 407-427 aa
Cytoplasmic: 428-519 aa
Helical: 520-540 aa
Extracellular: 541-568 aa
Helical: 569-589 aa
Cytoplasmic: 590-607 aa
Helical: 608-628 aa
Extracellular: 629-657 aa
Helical: 658-678 aa
Cytoplasmic: 679-725 aa
Helical: 726-746 aa
Helical: 747-767 aa
Cytoplasmic: 768-784 aa
Helical: 785-805 aa
Extracellular: 806-892 aa
Helical: 893-913 aa
Cytoplasmic: 914-986 aa

Wang, T., Wang, H., Yang, F., Gao, K., Luo, S., Bai, L., ... & Xiao, Q. (2021). Honokiol inhibits proliferation of colorectal cancer cells by targeting Anoctamin 1/TMEM16A Ca2+‐activated Cl‐channels. British Journal of Pharmacology.

Heng, J., Wu, D., Lu, S., & Zhao, Y. (2020). miR-106a Targets Anoctamin 1 (ANO1) to Regulate Lipopolysaccharide (LPS)-Induced Inflammatory Response in Macrophages. Medical Science Monitor: International Medical Journal of Experimental and Clinical Research, 26, e922479-1.

Vanoni, S., Zeng, C., Marella, S., Uddin, J., Wu, D., Arora, K., ... & Hogan, S. P. (2020). Identification of anoctamin 1 (ANO1) as a key driver of esophageal epithelial proliferation in eosinophilic esophagitis. Journal of Allergy and Clinical Immunology, 145(1), 239-254.

Dulin, N. O. (2020). Calcium-activated chloride channel ANO1/TMEM16A: regulation of expression and signaling. Frontiers in Physiology, 11.

Mazzone, A., Gibbons, S. J., Eisenman, S. T., Strege, P. R., Zheng, T., D'Amato, M., ... & Farrugia, A. G. (2019). Direct repression of anoctamin 1 (ANO1) gene transcription by Gli proteins. The FASEB Journal, 33(5), 6632-6642.

Park, Y. R., Lee, S. T., Kim, S. L., Zhu, S. M., Lee, M. R., Kim, S. H., ... & Kim, S. W. (2019). Down-regulation of miR-9 promotes epithelial mesenchymal transition via regulating anoctamin-1 (ANO1) in CRC cells. Cancer genetics, 231, 22-31.

Lee, B., Hong, G. S., Lee, S. H., Kim, H., Kim, A., Hwang, E. M., ... & Oh, U. (2019). Anoctamin 1/TMEM16A controls intestinal Cl− secretion induced by carbachol and cholera toxin. Experimental & molecular medicine, 51(8), 1-14.

Allawzi, A. M., Vang, A., Clements, R. T., Jhun, B. S., Kue, N. R., Mancini, T. J., ... & Choudhary, G. (2018). Activation of anoctamin-1 limits pulmonary endothelial cell proliferation via p38–Mitogen-activated protein Kinase–dependent apoptosis. American journal of respiratory cell and molecular biology, 58(5), 658-667.

Hahn, A., Salomon, J. J., Leitz, D., Feigenbutz, D., Korsch, L., Lisewski, I., ... & Möhrlen, F. (2018). Expression and function of Anoctamin 1/TMEM16A calcium-activated chloride channels in airways of in vivo mouse models for cystic fibrosis research. Pflügers Archiv-European Journal of Physiology, 470(9), 1335-1348.

He, Y., Li, H., Chen, Y., Li, P., Gao, L., Zheng, Y., ... & Qian, X. (2017). Expression of anoctamin 1 is associated with advanced tumor stage in patients with non-small cell lung cancer and predicts recurrence after surgery. Clinical and Translational Oncology, 19(9), 1091-1098.

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For research use only. Not intended for any clinical use.

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