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Mouse Anti-ANP32A Recombinant Antibody (CBYJL-017) (CBMAB-L0042-YJ)

Provided herein is a Mouse monoclonal antibody, which binds to Acidic Nuclear Phosphoprotein 32 Family Member A (ANP32A). The antibody can be used for immunoassay techniques, such as ICC, IP, WB.
See all ANP32A antibodies
Published Data
Mouse Anti-ANP32A Recombinant Antibody (CBYJL-017) in WB
RT-PCR analysis of the A99505 DF1 clone (left) and Western blot analysis ANP32A and ACTB protein expression in wild-type cells and in clones A99505 and AKO107 (right). ...View More
Park, Y. H., Chungu, K., Lee, S. B., Woo, S. J., Cho, H. Y., Lee, H. J., ... & Han, J. Y. (2020). Host-specific restriction of avian influenza virus caused by differential dynamics of ANP32 family members. The Journal of Infectious Diseases, 221(1), 71-80.
Mouse Anti-ANP32A Recombinant Antibody (CBYJL-017) in WB
Proteins that Increase in Failing and Non-Failing Human Hearts. Western blot analysis was determined as outlined in the methods. Proteins levels were normalized to β-actin. A. Acidic Nuclear Phosphoprotein 32A; B. Apolipoprotein A1-Preprotein; C. Nucleolar protein 3; D. Brain Creatine Kinase. Bands were analyzed using image J software and the relative density shown. NF, non-failing hearts; F, failing hearts. *p<0.05 compared to non-failing control. ...View More
Shi, T., Moravec, C. S., & Perez, D. M. (2013). Novel proteins associated with human dilated cardiomyopathy: Selective reduction in α1A-adrenergic receptors and increased desensitization proteins. Journal of Receptors and Signal Transduction, 33(2), 96-106.

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBYJL-017
Antibody Isotype
IgG1, κ
Application
WB, IP, IHC-P, IF, ELISA

Basic Information

Immunogen
Recombinant ANP32A of human origin.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:30-1:3,000
IHC-P1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.1 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Acidic Nuclear Phosphoprotein 32 Family Member A
Introduction
ANP32A is implicated in a number of cellular processes, including proliferation, caspase-dependent and caspase-independent apoptosis, differentiation, suppression of transformation (tumor suppressor), regulation of mRNA trafficking and stability in association with ELAVL1, inhibition of protein phosphatase 2A, and inhibition of acetyltransferases as part of the INHAT (inhibitor of histone acetyltransferases) complex. ANP32A is involved in E4F1-mediated transcriptional repression.
Entrez Gene ID
8125
UniProt ID
P39687
Alternative Names
Acidic Nuclear Phosphoprotein 32 Family Member A; Acidic (Leucine-Rich) Nuclear Phosphoprotein 32 Family; Member A; Potent Heat-Stable Protein Phosphatase 2A Inhibitor I1PP2A; Putative HLA-DR-Associated Protein I; Leucine-Rich Acidic Nuclear Protein; Acidic Nuclear Phosphoprotein Pp32; Mapmodulin; C15orf1; PHAP1; PHAPI; PP32
Function
Multifunctional protein that is involved in the regulation of many processes including tumor suppression, apoptosis, cell cycle progression or transcription (PubMed:16341127, PubMed:11360199, PubMed:18439902, PubMed:10400610). Promotes apoptosis by favouring the activation of caspase-9/CASP9 and allowing apoptosome formation (PubMed:18439902). In addition, plays a role in the modulation of histone acetylation and transcription as part of the INHAT (inhibitor of histone acetyltransferases) complex. Inhibits the histone-acetyltranferase activity of EP300/CREBBP (CREB-binding protein) and EP300/CREBBP-associated factor by histone masking (PubMed:11830591). Preferentially binds to unmodified histone H3 and sterically inhibiting its acetylation and phosphorylation leading to cell growth inhibition (PubMed:16341127). Participates in other biochemical processes such as regulation of mRNA nuclear-to-cytoplasmic translocation and stability by its association with ELAVL1 (Hu-antigen R) (PubMed:18180367). Plays a role in E4F1-mediated transcriptional repression as well as inhibition of protein phosphatase 2A (PubMed:15642345, PubMed:17557114).
(Microbial infection) Plays an essential role in influenza A, B and C viral genome replication (PubMed:32694517, PubMed:33045004, PubMed:33208942, PubMed:30666459). Mechanistically, mediates the assembly of the viral replicase asymmetric dimers composed of PB1, PB2 and PA via its N-terminal region (PubMed:33208942). Plays also an essential role in foamy virus mRNA export from the nucleus (PubMed:21159877).
Biological Process
Intracellular signal transduction Source: ProtInc
Nucleocytoplasmic transport Source: UniProtKB
Regulation of apoptotic process Source: GO_Central
Regulation of mRNA stability Source: Reactome
Cellular Location
Nucleus; Cytoplasm; Endoplasmic reticulum. Translocates to the cytoplasm during the process of neuritogenesis (By similarity). Shuttles between nucleus and cytoplasm.
PTM
Phosphorylated on serine residues, at least in part by casein kinase 2/CK2.
The N-terminus is blocked.
Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group (By similarity).

Park, Y. H., Woo, S. J., Chungu, K., Lee, S. B., Shim, J. H., Lee, H. J., ... & Han, J. Y. (2021). Asp149 and Asp152 in chicken and human ANP32A play an essential role in the interaction with influenza viral polymerase. The FASEB Journal, 35(6), e21630.

Zhang, C., Wei, Y., Zhang, Y., Zhang, J., Xing, Q., Zhou, M., & Shan, Y. (2021). Establishment of a CRISPR/Cas9-mediated ANP32A homozygous knockout human embryonic stem cell line. Stem Cell Research, 52, 102234.

Park, Y. H., Chungu, K., Lee, S. B., Woo, S. J., Cho, H. Y., Lee, H. J., ... & Han, J. Y. (2020). Host-specific restriction of avian influenza virus caused by differential dynamics of ANP32 family members. The Journal of infectious diseases, 221(1), 71-80.

Carrique, L., Fan, H., Walker, A. P., Keown, J. R., Sharps, J., Staller, E., ... & Grimes, J. M. (2020). Host ANP32A mediates the assembly of the influenza virus replicase. Nature, 587(7835), 638-643.

Huang, S., Huang, Z., Ma, C., Luo, L., Li, Y. F., Wu, Y. L., ... & Feng, C. (2020). Acidic leucine-rich nuclear phosphoprotein-32A expression contributes to adverse outcome in acute myeloid leukemia. Annals of translational medicine, 8(6).

Wei, X., Liu, Z., Wang, J., Yang, R., Yang, J., Guo, Y., ... & Liu, L. (2019). The interaction of cellular protein ANP32A with influenza A virus polymerase component PB2 promotes vRNA synthesis. Archives of virology, 164(3), 787-798.

Yang, X., Lu, B., Sun, X., Han, C., Fu, C., Xu, K., ... & Huang, Z. (2018). ANP32A regulates histone H3 acetylation and promotes leukemogenesis. Leukemia, 32(7), 1587-1597.

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For research use only. Not intended for any clinical use.

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