Mouse Anti-AOC3 Recombinant Antibody (CBYC-A641) (CBMAB-A2954-YC)

Name: Mouse Anti-AOC3 Recombinant Antibody (CBYC-A641)
SKU: CBMAB-A2954-YC
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-AOC3 Recombinant Antibody (CBYC-A641) in IF

Antibody staining of recombinant proteins expressed on the surface of CHO-745 cells. Immunofluorescence analyses (IFA) with non-permeabilized CHO-745 cells confirm surface localization of the overexpressed endothelial proteins. TNFR2sh, HRH1, VAP-1 (green) as well as untagged LOX1 expressed in CHO-745 cells were labelled with respective antibodies (red). No antibodies were available which are directed against any of the nine extracellular domains of MRP2. ...View More

Esser, C., Bachmann, A., Kuhn, D., Schuldt, K., Förster, B., Thiel, M., ... & Horstmann, R. D. (2014). Evidence of promiscuous endothelial binding by P lasmodium falciparum-infected erythrocytes. Cellular microbiology, 16(5), 701-708.

Mouse Anti-AOC3 Recombinant Antibody (CBYC-A641) in WB

Western blot analysis of adipose tissue from 6 wild type VAP-1 (WT) and 6 VAP-1Y471F mice. The upper panel shows the blot probed for VAP-1 and the lower panel shows the same blot which was probed for βTubulin. The VAP-1 and βTubulin bands are indicated with arrows in each case. The molecular weight standard is indicated by Mol. Wt. Stds (kDa) and the 98 and 49 kDa bands are highlighted. ...View More

Noonan, T., Lukas, S., Peet, G. W., Pelletier, J., Panzenbeck, M., Hanidu, A., ... & Modis, L. K. (2013). The oxidase activity of vascular adhesion protein-1 (VAP-1) is essential for function. American journal of clinical and experimental immunology, 2(2), 172.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBYC-A641

Application

WB, IF, FC

Immunogen

Affinity purified VAP-1 from tonsil stroma of human origin.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Amine Oxidase, Copper Containing 3

Introduction

AOC3 is a member of the semicarbazide-sensitive amine oxidase family. Copper amine oxidases catalyze the oxidative conversion of amines to aldehydes in the presence of copper and quinone cofactor. The encoded protein is localized to the cell surface, has

Entrez Gene ID

8639

UniProt ID

Q16853

Alternative Names

Amine Oxidase, Copper Containing 3; Semicarbazide-Sensitive Amine Oxidase; Vascular Adhesion Protein 1; Copper Amine Oxidase; EC 1.4.3.21; VAP-1; SSAO;

Function

Cell adhesion protein that participates in lymphocyte extravasation and recirculation by mediating the binding of lymphocytes to peripheral lymph node vascular endothelial cells in an L-selectin-independent fashion. Has semicarbazide-sensitive (SSAO) monoamine oxidase activity. May play a role in adipogenesis.

Biological Process

Amine metabolic process Source: UniProtKB
Cell adhesion Source: UniProtKB
Inflammatory response Source: UniProtKB
Negative regulation of primary amine oxidase activity Source: UniProtKB
Response to antibiotic Source: UniProtKB
Xenobiotic metabolic process Source: Reactome

Cellular Location

Cell membrane

Topology

Cytoplasmic: 1-5 aa
Helical: 6-26 aa
Extracellular: 27-763 aa

PTM

Topaquinone (TPQ) is generated by copper-dependent autoxidation of a specific tyrosyl residue.
N- and O-glycosylated.

Boyer, D. S., Rippmann, J. F., Ehrlich, M. S., Bakker, R. A., Chong, V., & Nguyen, Q. D. (2021). Amine oxidase copper-containing 3 (AOC3) inhibition: a potential novel target for the management of diabetic retinopathy. International Journal of Retina and Vitreous, 7(1), 1-12.

Chang, C. Y., Wu, K. L., Chang, Y. Y., Tsai, P. H., Hung, J. Y., Chang, W. A., ... & Hsu, Y. L. (2021). Amine oxidase, copper containing 3 exerts anti‑mesenchymal transformation and enhances CD4+ T‑cell recruitment to prolong survival in lung cancer. Oncology Reports, 46(3), 1-15.

Jargaud, V., Bour, S., Tercé, F., Collet, X., Valet, P., Bouloumié, A., ... & Carpéné, C. (2021). Obesity of mice lacking VAP-1/SSAO by Aoc3 gene deletion is reproduced in mice expressing a mutated vascular adhesion protein-1 (VAP-1) devoid of amine oxidase activity. Journal of physiology and biochemistry, 77(1), 141-154.

Romauch, M. (2019). Zinc-α2-glycoprotein as an inhibitor of amine oxidase copper-containing 3. Open biology, 10(4), 190035.

Yang, H., Ralle, M., Wolfgang, M. J., Dhawan, N., Burkhead, J. L., Rodriguez, S., ... & Lutsenko, S. (2018). Copper-dependent amino oxidase 3 governs selection of metabolic fuels in adipocytes. PLoS biology, 16(9), e2006519.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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