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Mouse Anti-AP1AR Recombinant Antibody (C-6) (CBMAB-A2958-YC)

Provided herein is a Mouse monoclonal antibody against Human Adaptor Related Protein Complex 1 Associated Regulatory Protein. The antibody can be used for immunoassay techniques, such as WB, IP, IF, ELISA.
See all AP1AR antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
C-6
Antibody Isotype
IgG1, κ
Application
WB, IP, IF, ELISA

Basic Information

Immunogen
Amino acids 131-302 mapping at the C-terminus of AP1AR of human origin.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:30-1:3,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Adaptor Related Protein Complex 1 Associated Regulatory Protein
Introduction
Necessary for adaptor protein complex 1 (AP-1)-dependent transport between the trans-Golgi network and endosomes. Regulates the membrane association of AP1G1/gamma1-adaptin, one of the subunits of the AP-1 adaptor complex. The direct interaction with AP1G
Entrez Gene ID
55435
UniProt ID
Q63HQ0
Alternative Names
Adaptor Related Protein Complex 1 Associated Regulatory Protein; Gamma-1-Adaptin Brefeldin A Resistance Protein; Gamma-A1-Adaptin And Kinesin Interactor; Gamma-BAR; C4orf16; Gadkin; 2C18; GBAR; Adaptor-Related Protein Complex 1 Associated Regulatory Prote
Function
Necessary for adaptor protein complex 1 (AP-1)-dependent transport between the trans-Golgi network and endosomes. Regulates the membrane association of AP1G1/gamma1-adaptin, one of the subunits of the AP-1 adaptor complex. The direct interaction with AP1G1/gamma1-adaptin attenuates the release of the AP-1 complex from membranes. Regulates endosomal membrane traffic via association with AP-1 and KIF5B thus linking kinesin-based plus-end-directed microtubular transport to AP-1-dependent membrane traffic. May act as effector of AP-1 in calcium-induced endo-lysosome secretion. Inhibits Arp2/3 complex function; negatively regulates cell spreading, size and motility via intracellular sequestration of the Arp2/3 complex.
Biological Process
Cellular protein localization Source: Ensembl
Negative regulation of cell motility Source: Ensembl
Negative regulation of receptor recycling Source: UniProtKB
Negative regulation of substrate adhesion-dependent cell spreading Source: MGI
Protein transport Source: UniProtKB-KW
Regulation of Arp2/3 complex-mediated actin nucleation Source: Ensembl
Vesicle targeting, trans-Golgi to endosome Source: UniProtKB
Cellular Location
Late endosome; Early endosome; trans-Golgi network. Localizes to the juxta-nuclear Golgi region and to tubular structures throughout the cytoplasm, which are highly mobile and cycle between the juxta-nuclear area and the cell periphery.
PTM
Palmitoylated.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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