Mouse Anti-AP1AR Recombinant Antibody (C-6) (CBMAB-A2958-YC)

Name: Mouse Anti-AP1AR Recombinant Antibody (C-6)
SKU: CBMAB-A2958-YC
Rating: 5 (1 reviews)

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Datasheet

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Basic Information

Host Animal

Mouse

Clone

C-6

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 131-302 mapping at the C-terminus of AP1AR of human origin.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:30-1:3,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Adaptor Related Protein Complex 1 Associated Regulatory Protein

Introduction

Entrez Gene ID

55435

UniProt ID

Q63HQ0

Alternative Names

Adaptor Related Protein Complex 1 Associated Regulatory Protein; Gamma-1-Adaptin Brefeldin A Resistance Protein; Gamma-A1-Adaptin And Kinesin Interactor; Gamma-BAR; C4orf16; Gadkin; 2C18; GBAR; Adaptor-Related Protein Complex 1 Associated Regulatory Prote

Function

Necessary for adaptor protein complex 1 (AP-1)-dependent transport between the trans-Golgi network and endosomes. Regulates the membrane association of AP1G1/gamma1-adaptin, one of the subunits of the AP-1 adaptor complex. The direct interaction with AP1G1/gamma1-adaptin attenuates the release of the AP-1 complex from membranes. Regulates endosomal membrane traffic via association with AP-1 and KIF5B thus linking kinesin-based plus-end-directed microtubular transport to AP-1-dependent membrane traffic. May act as effector of AP-1 in calcium-induced endo-lysosome secretion. Inhibits Arp2/3 complex function; negatively regulates cell spreading, size and motility via intracellular sequestration of the Arp2/3 complex.

Biological Process

Cellular protein localization Source: Ensembl
Negative regulation of cell motility Source: Ensembl
Negative regulation of receptor recycling Source: UniProtKB
Negative regulation of substrate adhesion-dependent cell spreading Source: MGI
Protein transport Source: UniProtKB-KW
Regulation of Arp2/3 complex-mediated actin nucleation Source: Ensembl
Vesicle targeting, trans-Golgi to endosome Source: UniProtKB

Cellular Location

Late endosome; Early endosome; trans-Golgi network. Localizes to the juxta-nuclear Golgi region and to tubular structures throughout the cytoplasm, which are highly mobile and cycle between the juxta-nuclear area and the cell periphery.

PTM

Palmitoylated.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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