Mouse Anti-AP1G1 Recombinant Antibody (CBLG1-3125) (CBMAB-G1260-LY)

Name: Mouse Anti-AP1G1 Recombinant Antibody (CBLG1-3125)
SKU: CBMAB-G1260-LY
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-AP1G1 Recombinant Antibody (CBLG1-3125) in Immunoblot

ZnT5 protein is concentrated into the fractions of secretory apparatus. The post-nuclear supernatant derived from HeLa cells was separated into 10 fractions by centrifugation on a 0.7-1.7 m sucrose gradient. Membrane protein from each fraction was analyzed by immunoblotting, and the distribution of ZnT5 in the gradient was compared with that of indicated proteins. Indicated proteins are markers of the following organelles: GM130 for Golgi apparatus, SEC23 for COPII vesicles, EEA1 for endosomes, γ1-adaptin for AP-1 vesicles, and Calreticulin for ER. The asterisk in the panel of GM130 indicates a nonspecific band. ...View More

Suzuki, T., Ishihara, K., Migaki, H., Matsuura, W., Kohda, A., Okumura, K., ... & Kambe, T. (2005). Zinc transporters, ZnT5 and ZnT7, are required for the activation of alkaline phosphatases, zinc-requiring enzymes that are glycosylphosphatidylinositol-anchored to the cytoplasmic membrane. Journal of Biological Chemistry, 280(1), 637-643.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBLG1-3125

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 653-821 of γ1-Adaptin of mouse origin.

Host Species

Mouse

Specificity

Mouse, Rat

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:30-1:3,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Adaptor Related Protein Complex 1 Gamma 1 Subunit

Introduction

Adaptins are important components of clathrin-coated vesicles transporting ligand-receptor complexes from the plasma membrane or from the trans-Golgi network to lysosomes. The adaptin family of proteins is composed of four classes of molecules named alpha, beta-, beta prime- and gamma- adaptins. Adaptins, together with medium and small subunits, form a heterotetrameric complex called an adaptor, whose role is to promote the formation of clathrin-coated pits and vesicles. The protein encoded by this gene is a gamma-adaptin protein and it belongs to the adaptor complexes large subunits family. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Entrez Gene ID

Human	164
Mouse	11765
Rat	171494

UniProt ID

Human	O43747
Mouse	P22892
Rat	B2RYN6

Function

Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules. In association with AFTPH/aftiphilin in the aftiphilin/p200/gamma-synergin complex, involved in the trafficking of transferrin from early to recycling endosomes, and the membrane trafficking of furin and the lysosomal enzyme cathepsin D between the trans-Golgi network (TGN) and endosomes (PubMed:15758025).

Biological Process

Antigen processing and presentation of exogenous peptide antigen via MHC class II Source: Reactome
Endosome to melanosome transport Source: ParkinsonsUK-UCL
Golgi to lysosome transport Source: UniProtKB
Golgi to vacuole transport Source: GO_Central
Intracellular protein transport Source: InterPro
Melanosome organization Source: ParkinsonsUK-UCL
Positive regulation of natural killer cell degranulation Source: UniProtKB
Positive regulation of natural killer cell mediated cytotoxicity Source: UniProtKB
Post-Golgi vesicle-mediated transport Source: Reactome
Receptor-mediated endocytosis Source: GO_Central
Regulation of defense response to virus by virus Source: Reactome

Cellular Location

Cytoplasm; Golgi apparatus; Clathrin-coated vesicle membrane; Perinuclear region; Clathrin-coated vesicle. Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex (PubMed:12773381). Co-localizes with AFTPH/aftiphilin in the cytoplasm (PubMed:15758025).

Usmani, M. A., Ahmed, Z. M., Pamela, M., Pienkowski, V. M., Rasmussen, K. J., Hernan, R., ... & Riazuddin, S. (2021). De novo and bi-allelic variants in AP1G1 cause neurodevelopmental disorder with developmental delay, intellectual disability, and epilepsy. The American Journal of Human Genetics.

Tao, X., Lu, Y., Qiu, S., Wang, Y., Qin, J., & Fan, Z. (2017). AP1G1 is involved in cetuximab-mediated downregulation of ASCT2-EGFR complex and sensitization of human head and neck squamous cell carcinoma cells to ROS-induced apoptosis. Cancer letters, 408, 33-42.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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