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Mouse Anti-AP1G1 Recombinant Antibody (CBLG1-3125) (CBMAB-G1260-LY)

This product is antibody recognizes AP1G1. The antibody CBLG1-3125 immunoassay techniques such as: WB.
See all AP1G1 antibodies
Published Data

Summary

Host Animal
Mouse
Specificity
Mouse, Rat
Clone
CBLG1-3125
Antibody Isotype
IgG2a, κ
Application
WB, IP, IF, ELISA

Basic Information

Immunogen
Amino acids 653-821 of γ1-Adaptin of mouse origin.
Host Species
Mouse
Specificity
Mouse, Rat
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:30-1:3,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Adaptor Related Protein Complex 1 Gamma 1 Subunit
Introduction
Adaptins are important components of clathrin-coated vesicles transporting ligand-receptor complexes from the plasma membrane or from the trans-Golgi network to lysosomes. The adaptin family of proteins is composed of four classes of molecules named alpha, beta-, beta prime- and gamma- adaptins. Adaptins, together with medium and small subunits, form a heterotetrameric complex called an adaptor, whose role is to promote the formation of clathrin-coated pits and vesicles. The protein encoded by this gene is a gamma-adaptin protein and it belongs to the adaptor complexes large subunits family. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Entrez Gene ID
Human164
Mouse11765
Rat171494
UniProt ID
HumanO43747
MouseP22892
RatB2RYN6
Function
Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules. In association with AFTPH/aftiphilin in the aftiphilin/p200/gamma-synergin complex, involved in the trafficking of transferrin from early to recycling endosomes, and the membrane trafficking of furin and the lysosomal enzyme cathepsin D between the trans-Golgi network (TGN) and endosomes (PubMed:15758025).
Biological Process
Antigen processing and presentation of exogenous peptide antigen via MHC class II Source: Reactome
Endosome to melanosome transport Source: ParkinsonsUK-UCL
Golgi to lysosome transport Source: UniProtKB
Golgi to vacuole transport Source: GO_Central
Intracellular protein transport Source: InterPro
Melanosome organization Source: ParkinsonsUK-UCL
Positive regulation of natural killer cell degranulation Source: UniProtKB
Positive regulation of natural killer cell mediated cytotoxicity Source: UniProtKB
Post-Golgi vesicle-mediated transport Source: Reactome
Receptor-mediated endocytosis Source: GO_Central
Regulation of defense response to virus by virus Source: Reactome
Cellular Location
Cytoplasm; Golgi apparatus; Clathrin-coated vesicle membrane; Perinuclear region; Clathrin-coated vesicle. Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex (PubMed:12773381). Co-localizes with AFTPH/aftiphilin in the cytoplasm (PubMed:15758025).
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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