Mouse Anti-APH1A Recombinant Antibody (665404) (CBMAB-1099-CN)

Name: Mouse Anti-APH1A Recombinant Antibody (665404)
SKU: CBMAB-1099-CN
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

665404

Application

IHC

Immunogen

Human APH1A synthetic peptide.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
IHC	8-25 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Lyophilized

Buffer

PBS, Trehalose

Preservative

None

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Aph-1 Homolog A, Gamma-Secretase Subunit

Introduction

This gene encodes a component of the gamma secretase complex that cleaves integral membrane proteins such as Notch receptors and beta-amyloid precursor protein. The gamma secretase complex contains this gene product, or the paralogous anterior pharynx defective 1 homolog B (APH1B), along with the presenilin, nicastrin, and presenilin enhancer-2 proteins. This is a non-catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (amyloid-beta precursor protein).

Entrez Gene ID

51107

UniProt ID

Q96BI3

Alternative Names

APH-1; APH-1A; CGI-78; 6530402N02Rik

Function

Non-catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (amyloid-beta precursor protein) (PubMed:12297508, PubMed:12522139, PubMed:12763021, PubMed:12679784, PubMed:25043039, PubMed:26280335, PubMed:30598546, PubMed:30630874). Required for normal gamma-secretase assembly (PubMed:12522139, PubMed:12471034, PubMed:12763021, PubMed:19369254). The gamma-secretase complex plays a role in Notch and Wnt signaling cascades and regulation of downstream processes via its role in processing key regulatory proteins, and by regulating cytosolic CTNNB1 levels (Probable).

Biological Process

Amyloid-beta formation Source: ARUK-UCL
Amyloid fibril formation Source: Reactome
Amyloid precursor protein catabolic process Source: ARUK-UCL
Amyloid precursor protein metabolic process Source: UniProtKB
Ephrin receptor signaling pathway Source: Reactome
Membrane protein ectodomain proteolysis Source: HGNC-UCL
Membrane protein intracellular domain proteolysis Source: UniProtKB
Metanephros development Source: Ensembl
Notch receptor processing Source: ARUK-UCL
Notch receptor processing, ligand-dependent Source: Reactome
Notch signaling pathway Source: GO_Central
Positive regulation of apoptotic process Source: Reactome
Positive regulation of catalytic activity Source: HGNC-UCL
Positive regulation of endopeptidase activity Source: ARUK-UCL
Protein processing Source: HGNC-UCL

Cellular Location

Golgi stack membrane; Endoplasmic reticulum membrane. Predominantly located in the endoplasmic reticulum and in the cis-Golgi.

Topology

Lumenal: 1-2 aa
Helical: 3-23 aa
Cytoplasmic: 24-31 aa
Helical: 32-52 aa
Lumenal: 53-68 aa
Helical: 69-89 aa
Cytoplasmic: 90-118 aa
Helical: 119-139 aa
Lumenal: 140-158 aa
Helical: 159-179 aa
Cytoplasmic: 180-186 aa
Helical: 187-207 aa
Lumenal: 208-213 aa
Helical: 214-234 aa
Cytoplasmic: 235-265 aa

Souma, R., Koyama, K., Koyama, A., Abe, S., Nakano, K., Yoshida, N., ... & Morioka, J. (2017). Polymorphisms of the aph-1 homolog A, gamma-secretase subunit (APH1A) gene in Japanese patients with aspirin-exacerbated respiratory disease (AERD).

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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