Human APH1A ELISA Kit (V2LY-0626-LY4606)

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Tested Data
Datasheet
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Basic Information

Sensitivity
0.0093 ng/mL
Detection Range
0.02-3.8 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
29.0 kDa
Components
  • Pre-coated ELISA Plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Aph-1 Homolog A, Gamma-Secretase Subunit
Function
Non-catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (amyloid-beta precursor protein) (PubMed:12297508, PubMed:12522139, PubMed:12763021, PubMed:12679784, PubMed:25043039, PubMed:26280335, PubMed:30598546, PubMed:30630874). Required for normal gamma-secretase assembly (PubMed:12522139, PubMed:12471034, PubMed:12763021, PubMed:19369254). The gamma-secretase complex plays a role in Notch and Wnt signaling cascades and regulation of downstream processes via its role in processing key regulatory proteins, and by regulating cytosolic CTNNB1 levels (Probable).
Biological Process
Amyloid-beta formation Source: ARUK-UCL
Amyloid fibril formation Source: Reactome
Amyloid precursor protein catabolic process Source: ARUK-UCL
Amyloid precursor protein metabolic process Source: UniProtKB
Ephrin receptor signaling pathway Source: Reactome
Membrane protein ectodomain proteolysis Source: HGNC-UCL
Membrane protein intracellular domain proteolysis Source: UniProtKB
Metanephros development Source: Ensembl
Notch receptor processing Source: ARUK-UCL
Notch receptor processing, ligand-dependent Source: Reactome
Notch signaling pathway Source: GO_Central
Positive regulation of apoptotic process Source: Reactome
Positive regulation of catalytic activity Source: HGNC-UCL
Positive regulation of endopeptidase activity Source: ARUK-UCL
Protein processing Source: HGNC-UCL
Cellular Location
Golgi stack membrane; Endoplasmic reticulum membrane. Predominantly located in the endoplasmic reticulum and in the cis-Golgi.
Topology
Lumenal: 1-2 aa
Helical: 3-23 aa
Cytoplasmic: 24-31 aa
Helical: 32-52 aa
Lumenal: 53-68 aa
Helical: 69-89 aa
Cytoplasmic: 90-118 aa
Helical: 119-139 aa
Lumenal: 140-158 aa
Helical: 159-179 aa
Cytoplasmic: 180-186 aa
Helical: 187-207 aa
Lumenal: 208-213 aa
Helical: 214-234 aa
Cytoplasmic: 235-265 aa

Souma, R., Koyama, K., Koyama, A., Abe, S., Nakano, K., Yoshida, N., ... & Morioka, J. (2017). Polymorphisms of the aph-1 homolog A, gamma-secretase subunit (APH1A) gene in Japanese patients with aspirin-exacerbated respiratory disease (AERD).

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Mouse Anti-APH1A Recombinant Antibody (665404) (CAT#: CBMAB-1099-CN)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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