Mouse Anti-APIP Recombinant Antibody (CBFYM-0864) (CBMAB-M1011-FY)

Name: Mouse Anti-APIP Recombinant Antibody (CBFYM-0864)
SKU: CBMAB-M1011-FY
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-APIP Recombinant Antibody (CBFYM-0864) in WB

Western blot analysis of the miRNA-transfected cells. Cell lysates were prepared two days after transfection. ...View More

Tran, P. X., Inoue, J., Harada, H., & Inazawa, J. (2022). Potential for reversing miR-634-mediated cytoprotective processes to improve efficacy of chemotherapy against oral squamous cell carcinoma. Molecular Therapy-Oncolytics, 24, 897-908.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYM-0864

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 141-179 within an internal region of APIP of human origin.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:30-1:3,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

APAF1 Interacting Protein

Introduction

APIP is an APAF1 -interacting protein that acts as a negative regulator of ischemic/hypoxic injury.

Entrez Gene ID

51074

UniProt ID

Q96GX9

Alternative Names

APAF1 Interacting Protein; Methylthioribulose-1-Phosphate Dehydratase; MTRu-1-P Dehydratase; HAPIP; Probable Methylthioribulose-1-Phosphate Dehydratase; APAF1-Interacting Protein

Function

Catalyzes the dehydration of methylthioribulose-1-phosphate (MTRu-1-P) into 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P). Functions in the methionine salvage pathway, which plays a key role in cancer, apoptosis, microbial proliferation and inflammation. May inhibit the CASP1-related inflammatory response (pyroptosis), the CASP9-dependent apoptotic pathway and the cytochrome c-dependent and APAF1-mediated cell death.

Biological Process

Apoptotic process Source: UniProtKB-KW
L-methionine salvage from methylthioadenosine Source: UniProtKB
L-methionine salvage from S-adenosylmethionine Source: UniProtKB-UniRule
Negative regulation of apoptotic process Source: UniProtKB
Protein homotetramerization Source: UniProtKB
Pyroptosis Source: UniProtKB
Regulation of ERK1 and ERK2 cascade Source: UniProtKB

Cellular Location

Cytoplasm

Lim, B., Jung, K., Gwon, Y., Oh, J. G., Roh, J. I., Hong, S. H., ... & Jung, Y. K. (2019). Cardioprotective role of APIP in myocardial infarction through ADORA2B. Cell death & disease, 10(7), 1-13.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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