Rabbit Anti-APLN Recombinant Antibody (BA0076) (CBMAB-0065CQ)

Name: Rabbit Anti-APLN Recombinant Antibody (BA0076)
SKU: CBMAB-0065CQ
Rating: 5 (1 reviews)

See all APLN antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

BA0076

Application

ELISA

Immunogen

Synthetic amidated peptide, from residues in Human Apelin

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

59% PBS, pH 7.2, 40% Glycerol, 0.5% BSA

Preservative

0.01% sodium azide

Concentration

Batch dependent

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Apelin

Introduction

Apelin is a neuropeptide expressed in the supraoptic and paraventricular nuclei that acts on specific receptors located on vasopressinergic neurons. The gene encodes a 77-amino acid polypeptide that includes a secretory signal sequence. Synthetic 36-amino acid apelin (apelin-36), corresponding to the most C-terminal 36 residues of preproapelin, caused the acidification of cells expressing transfected APJ, but not other receptors. The peptide fragments activate different tissue specific signaling pathways that regulate diverse biological functions including fluid homeostasis, cardiovascular function and insulin secretion. This protein also functions as a coreceptor for the human immunodeficiency virus 1.

Entrez Gene ID

8862

UniProt ID

Q9ULZ1

Alternative Names

Apelin; APJ Endogenous Ligand; APEL; Apelin, AGTRL1 Ligand; AGTRL1 Ligand; XNPEP2

Function

Endogenous ligand for the apelin receptor (APLNR) (PubMed:10525157). Drives internalization of the apelin receptor (By similarity). Apelin-36 dissociates more hardly than (pyroglu)apelin-13 from APLNR (By similarity). Hormone involved in the regulation of cardiac precursor cell movements during gastrulation and heart morphogenesis (By similarity). Has an inhibitory effect on cytokine production in response to T-cell receptor/CD3 cross-linking; the oral intake of apelin in the colostrum and the milk might therefore modulate immune responses in neonates (By similarity). Plays a role in early coronary blood vessels formation (By similarity). Mediates myocardial contractility in an ERK1/2-dependent manner (By similarity). May also have a role in the central control of body fluid homeostasis by influencing vasopressin release and drinking behavior (By similarity).
(Microbial infection) Endogenous ligand for the apelin receptor (APLNR), an alternative coreceptor with CD4 for HIV-1 infection (PubMed:11090199). Inhibits HIV-1 entry in cells coexpressing CD4 and APLNR (PubMed:11090199). Apelin-36 has a greater inhibitory activity on HIV infection than other synthetic apelin derivatives (PubMed:11090199).

Biological Process

Angiogenesis Source: UniProtKB-KW
Apelin receptor signaling pathway Source: UniProtKB
Coronary vasculature development Source: UniProtKB
Drinking behavior Source: UniProtKB
Gastrulation Source: UniProtKB-KW
G protein-coupled receptor signaling pathway Source: Reactome
Immune response Source: ProtInc
Lactation Source: ProtInc
Negative regulation of blood pressure Source: UniProtKB
Negative regulation of fibroblast growth factor receptor signaling pathway Source: BHF-UCL
Negative regulation of gene expression Source: BHF-UCL
Negative regulation of vascular associated smooth muscle cell proliferation Source: BHF-UCL
Negative regulation of vasoconstriction Source: Ensembl
Positive regulation of corticotropin-releasing hormone secretion Source: Ensembl
Positive regulation of corticotropin secretion Source: Ensembl
Positive regulation of G protein-coupled receptor internalization Source: UniProtKB
Positive regulation of heart contraction Source: UniProtKB
Positive regulation of heat generation Source: Ensembl
Positive regulation of phosphorylation Source: Ensembl
Positive regulation of pri-miRNA transcription by RNA polymerase II Source: BHF-UCL
Positive regulation of vascular endothelial cell proliferation Source: BHF-UCL
Regulation of respiratory gaseous exchange Source: Ensembl
Regulation of the force of heart contraction Source: Ensembl
Signal transduction Source: ProtInc
Viral process Source: UniProtKB-KW

Cellular Location

Secreted; Extracellular space. Abundantly secreted in the colostrum. Lower level in milk. Decreases rapidly within several days after parturition in milk, but is still detectable even in commercial milk.

PTM

Several active peptides may be produced by proteolytic processing of the peptide precursor.

Chen, L., Zhou, T., White, T., O'Brien, A., Chakraborty, S., Liangpunsakul, S., ... & Glaser, S. (2021). The Apelin–Apelin Receptor Axis Triggers Cholangiocyte Proliferation and Liver Fibrosis During Mouse Models of Cholestasis. Hepatology, 73(6), 2411-2428.

Suriyaprom, K., Pheungruang, B., Tungtrongchitr, R., & Orn-uma, Y. S. (2020). Relationships of apelin concentration and APLN T-1860C polymorphism with obesity in Thai children. BMC pediatrics, 20(1), 1-10.

Wang, Y., Liu, W., Xiao, Y., Yuan, H., Wang, F., Jiang, P., & Luo, Z. (2020). Association of apelin and apelin receptor polymorphisms with the risk of comorbid depression and anxiety in coronary heart disease patients. Frontiers in genetics, 11, 893.

Griffiths, P. R., Lolait, S. J., Bijabhai, A., O’Carroll-Lolait, A., Paton, J. F., & O’Carroll, A. M. (2020). Increased apelin receptor gene expression in the subfornical organ of spontaneously hypertensive rats. PloS one, 15(4), e0231844.

Tolkach, Y., Ellinger, J., Kremer, A., Esser, L., Müller, S. C., Stephan, C., ... & Hauser, S. (2019). Apelin and apelin receptor expression in renal cell carcinoma. British journal of cancer, 120(6), 633-639.

Mercati, F., Scocco, P., Maranesi, M., Acuti, G., Petrucci, L., Cocci, P., ... & Dall’Aglio, C. (2019). Apelin system detection in the reproductive apparatus of ewes grazing on semi-natural pasture. Theriogenology, 139, 156-166.

Wang, W., Shen, M., Fischer, C., Basu, R., Hazra, S., Couvineau, P., ... & Oudit, G. Y. (2019). Apelin protects against abdominal aortic aneurysm and the therapeutic role of neutral endopeptidase resistant apelin analogs. Proceedings of the National Academy of Sciences, 116(26), 13006-13015.

Elsehmawy, A. A. E. W., El-Toukhy, S. E., Seliem, N. M. A., Moustafa, R. S., & Mohammed, D. S. (2019). Apelin and chemerin as promising adipokines in children with type 1 diabetes mellitus. Diabetes, metabolic syndrome and obesity: targets and therapy, 12, 383.

Wang, C., Liu, X., Kong, D., Qin, X., Li, Y., Teng, X., & Huang, X. (2017). Apelin as a novel drug for treating preeclampsia. Experimental and Therapeutic Medicine, 14(6), 5917-5923.

Roche, J., Ramé, C., Reverchon, M., Mellouk, N., Rak, A., Froment, P., & Dupont, J. (2017). Apelin (APLN) regulates progesterone secretion and oocyte maturation in bovine ovarian cells. Reproduction, 153(5), 589-603.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Rabbit Anti-APLN Recombinant Antibody (BA0076)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Antibody Pairs

APLN Matched Antibody Pair (084) (CAT#: APMAB-084LY)

APLN Matched Antibody Pair (085) (CAT#: APMAB-085LY)

APLN Matched Antibody Pair (086) (CAT#: APMAB-086LY)

APLN Matched Antibody Pair (087) (CAT#: APMAB-087LY)

APLN Matched Antibody Pair (088) (CAT#: APMAB-088LY)

APLN Matched Antibody Pair (089) (CAT#: APMAB-089LY)

Related Products

APLN Matched Antibody Pair (084) (CAT#: APMAB-084LY)

Mouse Anti-APLN Recombinant Antibody (1A8-2A5) (CAT#: CBMAB-A0407-LY)

Mouse Anti-APLN Recombinant Antibody (2B6) (CAT#: CBMAB-A3076-YC)

APLN Matched Antibody Pair (088) (CAT#: APMAB-088LY)

Mouse Anti-APLN Recombinant Antibody (2A1-2D5) (CAT#: CBMAB-A3075-YC)

Mouse Anti-APLN Recombinant Antibody (CBYCX-101) (CAT#: CBMAB-X0002-YC)

APLN Matched Antibody Pair (086) (CAT#: APMAB-086LY)

APLN Matched Antibody Pair (087) (CAT#: APMAB-087LY)

APLN Matched Antibody Pair (089) (CAT#: APMAB-089LY)

APLN Matched Antibody Pair (085) (CAT#: APMAB-085LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry