Mouse Recombinant APLN protein, Fc Tag (V2LY-0526-LY7814)

Name: Mouse Recombinant APLN protein, Fc Tag
SKU: V2LY-0526-LY7814
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Mouse

Tag

Fc Tag

Protein Construction

This product is Mouse Recombinant APLN protein, Fc Tag consist of Amino Acid: 1-77 and predicts a molecular mass of 33.57 kDa.

Molecule Mass

33.57 kDa

Verified

HPLC

Sequence

Amino Acid: 1-77

Species

Mouse

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by SDS-PAGE.>95% as determined by SEC-HPLC.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Apelin

Function

Endogenous ligand for the apelin receptor (APLNR) (PubMed:10525157). Drives internalization of the apelin receptor (By similarity). Apelin-36 dissociates more hardly than (pyroglu)apelin-13 from APLNR (By similarity). Hormone involved in the regulation of cardiac precursor cell movements during gastrulation and heart morphogenesis (By similarity). Has an inhibitory effect on cytokine production in response to T-cell receptor/CD3 cross-linking; the oral intake of apelin in the colostrum and the milk might therefore modulate immune responses in neonates (By similarity). Plays a role in early coronary blood vessels formation (By similarity). Mediates myocardial contractility in an ERK1/2-dependent manner (By similarity). May also have a role in the central control of body fluid homeostasis by influencing vasopressin release and drinking behavior (By similarity).
(Microbial infection) Endogenous ligand for the apelin receptor (APLNR), an alternative coreceptor with CD4 for HIV-1 infection (PubMed:11090199). Inhibits HIV-1 entry in cells coexpressing CD4 and APLNR (PubMed:11090199). Apelin-36 has a greater inhibitory activity on HIV infection than other synthetic apelin derivatives (PubMed:11090199).

Biological Process

Angiogenesis Source: UniProtKB-KW
Apelin receptor signaling pathway Source: UniProtKB
Coronary vasculature development Source: UniProtKB
Drinking behavior Source: UniProtKB
Gastrulation Source: UniProtKB-KW
G protein-coupled receptor signaling pathway Source: Reactome
Immune response Source: ProtInc
Lactation Source: ProtInc
Negative regulation of blood pressure Source: UniProtKB
Negative regulation of fibroblast growth factor receptor signaling pathway Source: BHF-UCL
Negative regulation of gene expression Source: BHF-UCL
Negative regulation of vascular associated smooth muscle cell proliferation Source: BHF-UCL
Negative regulation of vasoconstriction Source: Ensembl
Positive regulation of corticotropin-releasing hormone secretion Source: Ensembl
Positive regulation of corticotropin secretion Source: Ensembl
Positive regulation of G protein-coupled receptor internalization Source: UniProtKB
Positive regulation of heart contraction Source: UniProtKB
Positive regulation of heat generation Source: Ensembl
Positive regulation of phosphorylation Source: Ensembl
Positive regulation of pri-miRNA transcription by RNA polymerase II Source: BHF-UCL
Positive regulation of vascular endothelial cell proliferation Source: BHF-UCL
Regulation of respiratory gaseous exchange Source: Ensembl
Regulation of the force of heart contraction Source: Ensembl
Signal transduction Source: ProtInc
Viral process Source: UniProtKB-KW

Cellular Location

Secreted; Extracellular space. Abundantly secreted in the colostrum. Lower level in milk. Decreases rapidly within several days after parturition in milk, but is still detectable even in commercial milk.

PTM

Several active peptides may be produced by proteolytic processing of the peptide precursor.

Chen, L., Zhou, T., White, T., O'Brien, A., Chakraborty, S., Liangpunsakul, S., ... & Glaser, S. (2021). The Apelin–Apelin Receptor Axis Triggers Cholangiocyte Proliferation and Liver Fibrosis During Mouse Models of Cholestasis. Hepatology, 73(6), 2411-2428.

Suriyaprom, K., Pheungruang, B., Tungtrongchitr, R., & Orn-uma, Y. S. (2020). Relationships of apelin concentration and APLN T-1860C polymorphism with obesity in Thai children. BMC pediatrics, 20(1), 1-10.

Wang, Y., Liu, W., Xiao, Y., Yuan, H., Wang, F., Jiang, P., & Luo, Z. (2020). Association of apelin and apelin receptor polymorphisms with the risk of comorbid depression and anxiety in coronary heart disease patients. Frontiers in genetics, 11, 893.

Griffiths, P. R., Lolait, S. J., Bijabhai, A., O’Carroll-Lolait, A., Paton, J. F., & O’Carroll, A. M. (2020). Increased apelin receptor gene expression in the subfornical organ of spontaneously hypertensive rats. PloS one, 15(4), e0231844.

Tolkach, Y., Ellinger, J., Kremer, A., Esser, L., Müller, S. C., Stephan, C., ... & Hauser, S. (2019). Apelin and apelin receptor expression in renal cell carcinoma. British journal of cancer, 120(6), 633-639.

Mercati, F., Scocco, P., Maranesi, M., Acuti, G., Petrucci, L., Cocci, P., ... & Dall’Aglio, C. (2019). Apelin system detection in the reproductive apparatus of ewes grazing on semi-natural pasture. Theriogenology, 139, 156-166.

Wang, W., Shen, M., Fischer, C., Basu, R., Hazra, S., Couvineau, P., ... & Oudit, G. Y. (2019). Apelin protects against abdominal aortic aneurysm and the therapeutic role of neutral endopeptidase resistant apelin analogs. Proceedings of the National Academy of Sciences, 116(26), 13006-13015.

Elsehmawy, A. A. E. W., El-Toukhy, S. E., Seliem, N. M. A., Moustafa, R. S., & Mohammed, D. S. (2019). Apelin and chemerin as promising adipokines in children with type 1 diabetes mellitus. Diabetes, metabolic syndrome and obesity: targets and therapy, 12, 383.

Wang, C., Liu, X., Kong, D., Qin, X., Li, Y., Teng, X., & Huang, X. (2017). Apelin as a novel drug for treating preeclampsia. Experimental and Therapeutic Medicine, 14(6), 5917-5923.

Roche, J., Ramé, C., Reverchon, M., Mellouk, N., Rak, A., Froment, P., & Dupont, J. (2017). Apelin (APLN) regulates progesterone secretion and oocyte maturation in bovine ovarian cells. Reproduction, 153(5), 589-603.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

APLN Matched Antibody Pair (084) (CAT#: APMAB-084LY)

APLN Matched Antibody Pair (085) (CAT#: APMAB-085LY)

APLN Matched Antibody Pair (086) (CAT#: APMAB-086LY)

APLN Matched Antibody Pair (087) (CAT#: APMAB-087LY)

APLN Matched Antibody Pair (088) (CAT#: APMAB-088LY)

APLN Matched Antibody Pair (089) (CAT#: APMAB-089LY)

Antibody Pairs

APLN Matched Antibody Pair (084) (CAT#: APMAB-084LY)

APLN Matched Antibody Pair (085) (CAT#: APMAB-085LY)

APLN Matched Antibody Pair (086) (CAT#: APMAB-086LY)

APLN Matched Antibody Pair (087) (CAT#: APMAB-087LY)

APLN Matched Antibody Pair (088) (CAT#: APMAB-088LY)

APLN Matched Antibody Pair (089) (CAT#: APMAB-089LY)

Related Products

APLN Matched Antibody Pair (084) (CAT#: APMAB-084LY)

APLN Matched Antibody Pair (085) (CAT#: APMAB-085LY)

APLN Matched Antibody Pair (089) (CAT#: APMAB-089LY)

Mouse Anti-APLN Recombinant Antibody (2B6) (CAT#: CBMAB-A3076-YC)

APLN Matched Antibody Pair (088) (CAT#: APMAB-088LY)

Mouse Anti-APLN Recombinant Antibody (2A1-2D5) (CAT#: CBMAB-A3075-YC)

Rabbit Anti-APLN Recombinant Antibody (BA0076) (CAT#: CBMAB-0065CQ)

APLN Matched Antibody Pair (087) (CAT#: APMAB-087LY)

Mouse Anti-APLN Recombinant Antibody (1A8-2A5) (CAT#: CBMAB-A0407-LY)

APLN Matched Antibody Pair (086) (CAT#: APMAB-086LY)

Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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