Human APLN ELISA Kit (V2LY-0626-LY2298)

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Tested Data
Datasheet
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Basic Information

Sensitivity
0.0035 ng/mL
Detection Range
0.007-1.5 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
8.6 kDa
Components
  • Pre-coated ELISA Plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Apelin
Function
Endogenous ligand for the apelin receptor (APLNR) (PubMed:10525157). Drives internalization of the apelin receptor (By similarity). Apelin-36 dissociates more hardly than (pyroglu)apelin-13 from APLNR (By similarity). Hormone involved in the regulation of cardiac precursor cell movements during gastrulation and heart morphogenesis (By similarity). Has an inhibitory effect on cytokine production in response to T-cell receptor/CD3 cross-linking; the oral intake of apelin in the colostrum and the milk might therefore modulate immune responses in neonates (By similarity). Plays a role in early coronary blood vessels formation (By similarity). Mediates myocardial contractility in an ERK1/2-dependent manner (By similarity). May also have a role in the central control of body fluid homeostasis by influencing vasopressin release and drinking behavior (By similarity).
(Microbial infection) Endogenous ligand for the apelin receptor (APLNR), an alternative coreceptor with CD4 for HIV-1 infection (PubMed:11090199). Inhibits HIV-1 entry in cells coexpressing CD4 and APLNR (PubMed:11090199). Apelin-36 has a greater inhibitory activity on HIV infection than other synthetic apelin derivatives (PubMed:11090199).
Biological Process
Angiogenesis Source: UniProtKB-KW
Apelin receptor signaling pathway Source: UniProtKB
Coronary vasculature development Source: UniProtKB
Drinking behavior Source: UniProtKB
Gastrulation Source: UniProtKB-KW
G protein-coupled receptor signaling pathway Source: Reactome
Immune response Source: ProtInc
Lactation Source: ProtInc
Negative regulation of blood pressure Source: UniProtKB
Negative regulation of fibroblast growth factor receptor signaling pathway Source: BHF-UCL
Negative regulation of gene expression Source: BHF-UCL
Negative regulation of vascular associated smooth muscle cell proliferation Source: BHF-UCL
Negative regulation of vasoconstriction Source: Ensembl
Positive regulation of corticotropin-releasing hormone secretion Source: Ensembl
Positive regulation of corticotropin secretion Source: Ensembl
Positive regulation of G protein-coupled receptor internalization Source: UniProtKB
Positive regulation of heart contraction Source: UniProtKB
Positive regulation of heat generation Source: Ensembl
Positive regulation of phosphorylation Source: Ensembl
Positive regulation of pri-miRNA transcription by RNA polymerase II Source: BHF-UCL
Positive regulation of vascular endothelial cell proliferation Source: BHF-UCL
Regulation of respiratory gaseous exchange Source: Ensembl
Regulation of the force of heart contraction Source: Ensembl
Signal transduction Source: ProtInc
Viral process Source: UniProtKB-KW
Cellular Location
Secreted; Extracellular space. Abundantly secreted in the colostrum. Lower level in milk. Decreases rapidly within several days after parturition in milk, but is still detectable even in commercial milk.
PTM
Several active peptides may be produced by proteolytic processing of the peptide precursor.

Chen, L., Zhou, T., White, T., O'Brien, A., Chakraborty, S., Liangpunsakul, S., ... & Glaser, S. (2021). The Apelin–Apelin Receptor Axis Triggers Cholangiocyte Proliferation and Liver Fibrosis During Mouse Models of Cholestasis. Hepatology, 73(6), 2411-2428.

Suriyaprom, K., Pheungruang, B., Tungtrongchitr, R., & Orn-uma, Y. S. (2020). Relationships of apelin concentration and APLN T-1860C polymorphism with obesity in Thai children. BMC pediatrics, 20(1), 1-10.

Wang, Y., Liu, W., Xiao, Y., Yuan, H., Wang, F., Jiang, P., & Luo, Z. (2020). Association of apelin and apelin receptor polymorphisms with the risk of comorbid depression and anxiety in coronary heart disease patients. Frontiers in genetics, 11, 893.

Griffiths, P. R., Lolait, S. J., Bijabhai, A., O’Carroll-Lolait, A., Paton, J. F., & O’Carroll, A. M. (2020). Increased apelin receptor gene expression in the subfornical organ of spontaneously hypertensive rats. PloS one, 15(4), e0231844.

Tolkach, Y., Ellinger, J., Kremer, A., Esser, L., Müller, S. C., Stephan, C., ... & Hauser, S. (2019). Apelin and apelin receptor expression in renal cell carcinoma. British journal of cancer, 120(6), 633-639.

Mercati, F., Scocco, P., Maranesi, M., Acuti, G., Petrucci, L., Cocci, P., ... & Dall’Aglio, C. (2019). Apelin system detection in the reproductive apparatus of ewes grazing on semi-natural pasture. Theriogenology, 139, 156-166.

Wang, W., Shen, M., Fischer, C., Basu, R., Hazra, S., Couvineau, P., ... & Oudit, G. Y. (2019). Apelin protects against abdominal aortic aneurysm and the therapeutic role of neutral endopeptidase resistant apelin analogs. Proceedings of the National Academy of Sciences, 116(26), 13006-13015.

Elsehmawy, A. A. E. W., El-Toukhy, S. E., Seliem, N. M. A., Moustafa, R. S., & Mohammed, D. S. (2019). Apelin and chemerin as promising adipokines in children with type 1 diabetes mellitus. Diabetes, metabolic syndrome and obesity: targets and therapy, 12, 383.

Wang, C., Liu, X., Kong, D., Qin, X., Li, Y., Teng, X., & Huang, X. (2017). Apelin as a novel drug for treating preeclampsia. Experimental and Therapeutic Medicine, 14(6), 5917-5923.

Roche, J., Ramé, C., Reverchon, M., Mellouk, N., Rak, A., Froment, P., & Dupont, J. (2017). Apelin (APLN) regulates progesterone secretion and oocyte maturation in bovine ovarian cells. Reproduction, 153(5), 589-603.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Recombinant APLN protein, Fc Tag (CAT#: V2LY-0526-LY7814)

Mouse Apln ELISA Kit (CAT#: V2LY-0626-LY1308)

Rat Apln ELISA Kit (CAT#: V2LY-0626-LY1331)

Cattle APLN ELISA Kit (CAT#: V2LY-0626-LY505)

APLN Matched Antibody Pair (084) (CAT#: APMAB-084LY)

APLN Matched Antibody Pair (085) (CAT#: APMAB-085LY)

APLN Matched Antibody Pair (086) (CAT#: APMAB-086LY)

APLN Matched Antibody Pair (087) (CAT#: APMAB-087LY)

APLN Matched Antibody Pair (088) (CAT#: APMAB-088LY)

APLN Matched Antibody Pair (089) (CAT#: APMAB-089LY)

Antibody Pairs

APLN Matched Antibody Pair (084) (CAT#: APMAB-084LY)

APLN Matched Antibody Pair (085) (CAT#: APMAB-085LY)

APLN Matched Antibody Pair (086) (CAT#: APMAB-086LY)

APLN Matched Antibody Pair (087) (CAT#: APMAB-087LY)

APLN Matched Antibody Pair (088) (CAT#: APMAB-088LY)

APLN Matched Antibody Pair (089) (CAT#: APMAB-089LY)

Related Products

APLN Matched Antibody Pair (086) (CAT#: APMAB-086LY)

Mouse Anti-APLN Recombinant Antibody (1A8-2A5) (CAT#: CBMAB-A0407-LY)

Mouse Recombinant APLN protein, Fc Tag (CAT#: V2LY-0526-LY7814)

Mouse Anti-APLN Recombinant Antibody (2B6) (CAT#: CBMAB-A3076-YC)

APLN Matched Antibody Pair (087) (CAT#: APMAB-087LY)

APLN Matched Antibody Pair (089) (CAT#: APMAB-089LY)

Mouse Anti-APLN Recombinant Antibody (2A1-2D5) (CAT#: CBMAB-A3075-YC)

APLN Matched Antibody Pair (084) (CAT#: APMAB-084LY)

Rabbit Anti-APLN Recombinant Antibody (BA0076) (CAT#: CBMAB-0065CQ)

APLN Matched Antibody Pair (088) (CAT#: APMAB-088LY)

Isotype control

For research use only. Not intended for any clinical use.

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