Mouse Anti-APOC2 Recombinant Antibody (3E4) (CBMAB-A3194-YC)

Provided herein is a Mouse monoclonal antibody against Human Apolipoprotein C2. The antibody can be used for immunoassay techniques, such as ELISA, WB.
See all APOC2 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

3E4

Antibody Isotype

IgG2a, κ

Application

ELISA, WB

Basic Information

Immunogen

Partial recombinant corresponding to aa 23-101 from human APOC2 (AAH05348) with GST tag.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4

Preservative

None

Concentration

Batch dependent

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

apolipoprotein C-II

Introduction

APOC2 is a lipid-binding protein belonging to the apolipoprotein gene family. The protein is secreted in plasma where it is a component of very low density lipoprotein. This protein activates the enzyme lipoprotein lipase, which hydrolyzes triglycerides a

Entrez Gene ID

344

UniProt ID

P02655

Alternative Names

Apolipoprotein C2; Apolipoprotein C-II; APO-CII; APOC-II; APC2;

Function

Component of chylomicrons, very low-density lipoproteins (VLDL), low-density lipoproteins (LDL), and high-density lipoproteins (HDL) in plasma. Plays an important role in lipoprotein metabolism as an activator of lipoprotein lipase. Both proapolipoprotein C-II and apolipoprotein C-II can activate lipoprotein lipase. In normolipidemic individuals, it is mainly distributed in the HDL, whereas in hypertriglyceridemic individuals, predominantly found in the VLDL and LDL.

Biological Process

Cholesterol efflux Source: BHF-UCL
Cholesterol homeostasis Source: BHF-UCL
Chylomicron assembly Source: Reactome
Chylomicron remnant clearance Source: BHF-UCL
Chylomicron remodeling Source: Reactome
High-density lipoprotein particle clearance Source: BHF-UCL
High-density lipoprotein particle remodeling Source: Reactome
Intracellular receptor signaling pathway Source: Reactome
Lipid catabolic process Source: UniProtKB-KW
Negative regulation of cholesterol transport Source: BHF-UCL
Negative regulation of lipid metabolic process Source: BHF-UCL
Negative regulation of receptor-mediated endocytosis Source: BHF-UCL
Negative regulation of very-low-density lipoprotein particle clearance Source: BHF-UCL
Phospholipid efflux Source: BHF-UCL
Positive regulation of fatty acid biosynthetic process Source: BHF-UCL
Positive regulation of lipoprotein lipase activity Source: BHF-UCL
Positive regulation of phospholipase activity Source: BHF-UCL
Positive regulation of phospholipid catabolic process Source: BHF-UCL
Positive regulation of triglyceride catabolic process Source: BHF-UCL
Positive regulation of very-low-density lipoprotein particle remodeling Source: BHF-UCL
Retinoid metabolic process Source: Reactome
Reverse cholesterol transport Source: BHF-UCL
Triglyceride homeostasis Source: BHF-UCL
Triglyceride-rich lipoprotein particle remodeling Source: BHF-UCL
Very-low-density lipoprotein particle remodeling Source: BHF-UCL

Cellular Location

Secreted

Involvement in disease

Hyperlipoproteinemia 1B (HLPP1B): Autosomal recessive trait characterized by hypertriglyceridemia, xanthomas, and increased risk of pancreatitis and early atherosclerosis.

PTM

Proapolipoprotein C-II is synthesized as a sialic acid containing glycoprotein which is subsequently desialylated prior to its proteolytic processing.
Proapolipoprotein C-II, the major form found in plasma undergoes proteolytic cleavage of its N-terminal hexapeptide to generate apolipoprotein C-II, which occurs as the minor form in plasma.

References

Liu, Y., Meng, Y., Zhang, T., & Alachkar, H. (2021). Deregulation of apolipoprotein C2 gene in cancer: A potential metabolic vulnerability. Clinical and Translational Medicine, 11(6).

Xenoulis, P. G., Tate, N. M., Bishop, M. A., Steiner, J. M., Suchodolski, J. S., & Furrow, E. (2020). Sequence analysis of the coding regions of the apolipoprotein C2 (APOC2) gene in Miniature Schnauzers with idiopathic hypertriglyceridemia. The Veterinary Journal, 265, 105559.

Zhang, T., Yang, J., Vaikari, V. P., Beckford, J. S., Wu, S., Akhtari, M., & Alachkar, H. (2020). Apolipoprotein C2-CD36 promotes leukemia growth and presents a targetable axis in acute myeloid leukemia. Blood cancer discovery, 1(2), 198.

Li, L., Xu, W., Fu, X., Huang, Y., Wen, Y., Xu, Q., ... & Lv, Z. (2020). Blood miR-1275 is associated with risk of ischemic stroke and inhibits macrophage foam cell formation by targeting ApoC2 gene. Gene, 731, 144364.

Lizunov, A. V., Okunevich, I. V., Pyotrovskiy, L. B., & Shabanov, P. D. (2020). Effects of kramizole on the expression of apo AI, APOC2, and SR-B1 genes in the hypercholesterol diet-induced rat model. Atherosclerosis, 315, e186-e187.

Yang, C., Tian, W., Ma, S., Guo, M., Lin, X., Gao, F., ... & Xian, X. (2020). AAV-Mediated ApoC2 gene therapy: reversal of severe hypertriglyceridemia and rescue of neonatal death in ApoC2-deficient hamsters. Molecular Therapy-Methods & Clinical Development, 18, 692-701.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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