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Mouse Anti-APOO Recombinant Antibody (2F1) (CBMAB-A3283-YC)

Provided herein is a Mouse monoclonal antibody against Human Apolipoprotein O. The antibody can be used for immunoassay techniques, such as ELISA, IHC, WB.
See all APOO antibodies
Published Data
Fig.1 GDP-exchange deficient ARF1 inhibits uptake of GPI-APs and the fluid-phase.
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Fig.2 The amount of ARF protein as detected on western blots is normalized to the actin level per lane, and expressed as a ratio with respect to the value obtained in mock-transfected sample.
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Fig.3 ASAP1 BARPZA alone or the BARPZA and GSTFIP3C complexes were immobilized on Ni-beads and then incubated with GTPS-loaded GFP-Rab11, Arf1-HA, Arf5-HA, or Arf6-HA.
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Summary

Host Animal
Mouse
Specificity
Human, Mouse
Clone
2F1
Antibody Isotype
IgG1
Application
ELISA, IHC, WB

Basic Information

Immunogen
Purified recombinant fragment of ApoO expressed in E.coli.
Host Species
Mouse
Specificity
Human, Mouse
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
ELISA1:10,000
WB1:500-1:2,000
IHC1:200-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Ascites
Buffer
0.5% BSA, 50% Glycerol
Preservative
0.03% sodium azide
Concentration
Batch dependent
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Apolipoprotein O
Introduction
APOO belongs to the apolipoprotein family. Members of this protein family are involved in the transport and metabolism of lipids. The encoded protein associates with HDL, LDL and VLDL lipoproteins and is characterized by chondroitin-sulfate glycosylation.
Entrez Gene ID
Human79135
Mouse68316
UniProt ID
HumanQ9BUR5
MouseQ9DCZ4
Alternative Names
Apolipoprotein O; Family With Sequence Similarity 121B; MICOS Complex Subunit MIC23; FAM121B; Mic23; MIC26;
Function
Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane. Plays a crucial role in crista junction formation and mitochondrial function (PubMed:25764979). Can promote cardiac lipotoxicity by enhancing mitochondrial respiration and fatty acid metabolism in cardiac myoblasts (PubMed:24743151). Promotes cholesterol efflux from macrophage cells. Detected in HDL, LDL and VLDL. Secreted by a microsomal triglyceride transfer protein (MTTP)-dependent mechanism, probably as a VLDL-associated protein that is subsequently transferred to HDL (PubMed:16956892).
Biological Process
Cristae formation Source: UniProtKB
Inner mitochondrial membrane organization Source: UniProtKB
Lipid transport Source: UniProtKB-KW
Cellular Location
Endoplasmic reticulum membrane; Secreted; Golgi apparatus membrane; Mitochondrion inner membrane; Mitochondrion. Exists in three distinct forms: a glycosylated and secreted form, an ER/Golgi-resident form and a non-glycosylated mitochondrial form.
Topology
Helical: 108-128 aa
PTM
O-glycosylation; glycosaminoglycan of chondroitin-sulfate type.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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