Mouse Anti-ApoO Recombinant Antibody (CBT4651) (V2LY-0625-LY2248)

Name: Mouse Anti-ApoO Recombinant Antibody (CBT4651)
SKU: V2LY-0625-LY2248
Rating: 5 (1 reviews)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human, Mouse

Clone

CBT4651

Antibody Isotype

IgG1

Application

WB, IHC

Basic Information

Immunogen

Purified recombinant fragment of ApoO expressed in E. Coli.

Host Species

Mouse

Specificity

Human, Mouse

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
IHC-P	1:200-1:1,000
ELISA	1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Supernatant

Preservative

Sodium azide

Concentration

Batch dependent

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

Apolipoprotein O

Entrez Gene ID

79135

UniProt ID

Q9BUR5

Function

Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane. Plays a crucial role in crista junction formation and mitochondrial function (PubMed:25764979). Can promote cardiac lipotoxicity by enhancing mitochondrial respiration and fatty acid metabolism in cardiac myoblasts (PubMed:24743151). Promotes cholesterol efflux from macrophage cells. Detected in HDL, LDL and VLDL. Secreted by a microsomal triglyceride transfer protein (MTTP)-dependent mechanism, probably as a VLDL-associated protein that is subsequently transferred to HDL (PubMed:16956892).

Biological Process

Cristae formation Source: UniProtKB
Inner mitochondrial membrane organization Source: UniProtKB
Lipid transport Source: UniProtKB-KW

Cellular Location

Endoplasmic reticulum membrane; Secreted; Golgi apparatus membrane; Mitochondrion inner membrane; Mitochondrion. Exists in three distinct forms: a glycosylated and secreted form, an ER/Golgi-resident form and a non-glycosylated mitochondrial form.

Topology

Helical: 108-128 aa

PTM

O-glycosylation; glycosaminoglycan of chondroitin-sulfate type.

More Infomation

References

Ghanbari Niaki, A., Aghababaeian, A., Joshaghani, H., Vakili, M. A., & Hoseini, M. (2019). Effect of Various Intensities of Circuit Resistance Training on Plasma Levels of High-Density Lipoprotein-Associated Apolipoprotein O, Total Cholesterol and Triglyceride in Untrained Men. Medical Laboratory Journal, 13(4), 23-28.

Laggner, H., Weijler, A. M., Schmidinger, B., Kapiotis, S., & Hermann, M. (2018). Oleic acid induces the novel Apolipoprotein O and reduces mitochondrial membrane potential in chicken and human hepatoma cells. Free Radical Biology and Medicine, 120, S108-S109.

Montasser, M. E., O’Hare, E. A., Wang, X., Howard, A. D., McFarland, R., Perry, J. A., ... & Chang, Y. P. C. (2018). An APOO pseudogene on chromosome 5q is associated with low-density lipoprotein cholesterol levels. Circulation, 138(13), 1343-1355.

Tian, F., Wu, C. L., Yu, B. L., Liu, L., & Hu, J. R. (2017). Apolipoprotein O expression in mouse liver enhances hepatic lipid accumulation by impairing mitochondrial function. Biochemical and biophysical research communications, 491(1), 8-14.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Related Products

Mouse Anti-APOO Recombinant Antibody (2F1)

Mouse Anti-APOO Recombinant Antibody (CBT176)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Documents

Datasheet
SDS
Request for COA

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Second antibody and isotype control

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