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Rabbit Anti-AQP5 Recombinant Antibody (CBYC-A725) (CBMAB-A3366-YC)

Provided herein is a Rabbit monoclonal antibody against Human Aquaporin 5. The antibody can be used for immunoassay techniques, such as ICC, IF, IHC, IHC-P, WB.
See all AQP5 antibodies

Summary

Host Animal
Rabbit
Specificity
Human
Clone
CBYC-A725
Antibody Isotype
IgG
Application
ICC, IF, IHC, WB

Basic Information

Immunogen
A synthetic peptide corresponding to residues within Human Aquaporin 5
Host Species
Rabbit
Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:1,000-1:2,000
IF(ICC)1:50-1:200
IHC-P1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.4, 150mM NaCl, 50% glycerol
Preservative
0.02% sodium azide
Concentration
Batch dependent
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Aquaporin 5
Introduction
Aquaporin 5 (AQP5) is a water channel protein. Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein (MIP or AQP0). Aquaporin 5 plays a role in the generation of saliva, tears and pulmonary secretions. AQP0, AQ
Entrez Gene ID
362
UniProt ID
P55064
Alternative Names
Aquaporin 5; AQP-5; Aquaporin-5; PPKB;
Function
Forms a water-specific channel (PubMed:8621489, PubMed:18768791).
Plays an important role in fluid secretion in salivary glands (By similarity).
Required for TRPV4 activation by hypotonicity. Together with TRPV4, controls regulatory volume decrease in salivary epithelial cells (PubMed:16571723).
Seems to play a redundant role in water transport in the eye, lung and in sweat glands (By similarity).
Biological Process
Camera-type eye morphogenesis Source: Ensembl
Carbon dioxide transport Source: UniProtKB
Cellular hypotonic response Source: UniProtKB
Odontogenesis Source: UniProtKB
Pancreatic juice secretion Source: UniProtKB
Protein homotetramerization Source: UniProtKB
Saliva secretion Source: Ensembl
Water transport Source: UniProtKB
Cellular Location
Apical cell membrane; Cell membrane; Cytoplasmic vesicle membrane. Hypotonicity increases location at the cell membrane. Phosphorylation decreases location at the cell membrane.
Involvement in disease
Keratoderma, palmoplantar, Bothnian type (PPKB): A dermatological disorder characterized by diffuse non-epidermolytic hyperkeratosis of the skin of palms and soles. PPKB is frequently complicated by fungal infections.
Topology
Cytoplasmic: 1-12 aa
Helical: 13-33 aa
Extracellular: 34-39 aa
Helical: 40-60 aa
Cytoplasmic: 61-65 aa
Discontinuously helical: 66-74 aa
Cytoplasmic: 75-87 aa
Helical: 88-108 aa
Extracellular: 109-126 aa
Helical: 127-147 aa
Cytoplasmic: 148-158 aa
Helical: 159-179 aa
Extracellular: 180 aa
Discontinuously helical: 181-191 aa
Extracellular: 192-203 aa
Helical: 204-224 aa
Cytoplasmic: 225-265 aa

Login, F. H., Palmfeldt, J., Cheah, J. S., Yamada, S., & Nejsum, L. N. (2021). Aquaporin-5 regulation of cell–cell adhesion proteins: an elusive “tail” story. American Journal of Physiology-Cell Physiology, 320(3), C282-C292.

Fan, Y., Song, T. R., Wei, Q., Yang, L., Lin, T., Feng, X. B., ... & Feng, S. J. (2021). Modulatory effect of aquaporin 5 on estrogen-induced epithelial-mesenchymal transition in prostate epithelial cells. Chinese medical journal, 134(4), 448.

Elkhider, A., Wang, B., Ouyang, X., Al‑Azab, M., Walana, W., Sun, X., ... & Li, X. (2020). Aquaporin 5 promotes tumor migration and angiogenesis in non‑small cell lung cancer cell line H1299. Oncology letters, 19(3), 1665-1672.

Rodrigues, C., Pimpão, C., Mósca, A. F., Coxixo, A. S., Lopes, D., da Silva, I. V., ... & Soveral, G. (2019). Human aquaporin-5 facilitates hydrogen peroxide permeation affecting adaption to oxidative stress and cancer cell migration. Cancers, 11(7), 932.

Rump, K., Unterberg, M., Dahlke, A., Nowak, H., Koos, B., Bergmann, L., ... & Rahmel, T. (2019). DNA methylation of a NF-κB binding site in the aquaporin 5 promoter impacts on mortality in sepsis. Scientific reports, 9(1), 1-11.

Lee, S. Y., Ha, E. J., Cho, H. W., Kim, H. R., Lee, D., & Eom, Y. B. (2019). Potential forensic application of receptor for advanced glycation end products (RAGE) and aquaporin 5 (AQP5) as novel biomarkers for diagnosis of drowning. Journal of forensic and legal medicine, 62, 56-62.

Kumari, S. S., Varadaraj, M., Menon, A. G., & Varadaraj, K. (2018). Aquaporin 5 promotes corneal wound healing. Experimental eye research, 172, 152-158.

Chen, C., Ma, T., Zhang, C., Zhang, H., Bai, L., Kong, L., & Luo, J. (2017). Down‐regulation of aquaporin 5‐mediated epithelial‐mesenchymal transition and anti‐metastatic effect by natural product Cairicoside E in colorectal cancer. Molecular carcinogenesis, 56(12), 2692-2705.

Direito, I., Paulino, J., Vigia, E., Brito, M. A., & Soveral, G. (2017). Differential expression of aquaporin‐3 and aquaporin‐5 in pancreatic ductal adenocarcinoma. Journal of surgical oncology, 115(8), 980-996.

Flodby, P., Li, C., Liu, Y., Wang, H., Rieger, M. E., Minoo, P., ... & Zhou, B. (2017). Cell-specific expression of aquaporin-5 (Aqp5) in alveolar epithelium is directed by GATA6/Sp1 via histone acetylation. Scientific reports, 7(1), 1-12.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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