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Mouse Anti-AQP5 Recombinant Antibody (D-7) (CBMAB-A3365-YC)

Provided herein is a Mouse monoclonal antibody against Human Aquaporin 5. The antibody can be used for immunoassay techniques, such as WB, IP, IF, ELISA.
See all AQP5 antibodies
Published Data
Mouse Anti-AQP5 Recombinant Antibody (D-7) in WB
The expression pattern of AQP5 analyzed by Western blot analysis. ...View More
Hu, S., Chen, B., Zhou, J., Liu, F., Mao, T., Pathak, J. L., ... & Li, J. (2023). Dental pulp stem cell-derived exosomes revitalize salivary gland epithelial cell function in NOD mice via the GPER-mediated cAMP/PKA/CREB signaling pathway. Journal of Translational Medicine, 21(1), 1-16.
Mouse Anti-AQP5 Recombinant Antibody (D-7) in WB
The expression levels of aquaporin-5 (AQP5) and E-cadherin (E-cad) were monitored in the submandibular gland of WT (n = 4) and Negr1 KO mice (n = 4). ...View More
Lee, J., Kim, S., Lee, B., Kim, Y. B., Kim, K. H., Chung, G., ... & Choi, S. Y. (2023). Major depression-related factor NEGR1 controls salivary secretion in mouse submandibular glands. Iscience, 26(5).

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
D-7
Antibody Isotype
IgG1, κ
Application
WB, IP, IF, ELISA, IHC-P

Basic Information

Immunogen
Amino acids 66-265 mapping at the C-terminus of AQP5 of human origin.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
IHC-P1:50-1:500
ELISA1:30-1:3,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Aquaporin 5
Introduction
Aquaporin 5 (AQP5) is a water channel protein. Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein (MIP or AQP0). Aquaporin 5 plays a role in the generation of saliva, tears and pulmonary secretions. AQP0, AQ
Entrez Gene ID
Human362
Mouse11830
Rat25241
UniProt ID
HumanP55087
MouseP55088
RatP47863
Alternative Names
Aquaporin 5; AQP-5; Aquaporin-5; PPKB;
Function
Forms a water-specific channel (PubMed:8621489, PubMed:18768791).
Plays an important role in fluid secretion in salivary glands (By similarity).
Required for TRPV4 activation by hypotonicity. Together with TRPV4, controls regulatory volume decrease in salivary epithelial cells (PubMed:16571723).
Seems to play a redundant role in water transport in the eye, lung and in sweat glands (By similarity).
Biological Process
Camera-type eye morphogenesis Source: Ensembl
Carbon dioxide transport Source: UniProtKB
Cellular hypotonic response Source: UniProtKB
Odontogenesis Source: UniProtKB
Pancreatic juice secretion Source: UniProtKB
Protein homotetramerization Source: UniProtKB
Saliva secretion Source: Ensembl
Water transport Source: UniProtKB
Cellular Location
Apical cell membrane; Cell membrane; Cytoplasmic vesicle membrane. Hypotonicity increases location at the cell membrane. Phosphorylation decreases location at the cell membrane.
Involvement in disease
Keratoderma, palmoplantar, Bothnian type (PPKB): A dermatological disorder characterized by diffuse non-epidermolytic hyperkeratosis of the skin of palms and soles. PPKB is frequently complicated by fungal infections.
Topology
Cytoplasmic: 1-12 aa
Helical: 13-33 aa
Extracellular: 34-39 aa
Helical: 40-60 aa
Cytoplasmic: 61-65 aa
Discontinuously helical: 66-74 aa
Cytoplasmic: 75-87 aa
Helical: 88-108 aa
Extracellular: 109-126 aa
Helical: 127-147 aa
Cytoplasmic: 148-158 aa
Helical: 159-179 aa
Extracellular: 180 aa
Discontinuously helical: 181-191 aa
Extracellular: 192-203 aa
Helical: 204-224 aa
Cytoplasmic: 225-265 aa

Login, F. H., Palmfeldt, J., Cheah, J. S., Yamada, S., & Nejsum, L. N. (2021). Aquaporin-5 regulation of cell–cell adhesion proteins: an elusive “tail” story. American Journal of Physiology-Cell Physiology, 320(3), C282-C292.

Fan, Y., Song, T. R., Wei, Q., Yang, L., Lin, T., Feng, X. B., ... & Feng, S. J. (2021). Modulatory effect of aquaporin 5 on estrogen-induced epithelial-mesenchymal transition in prostate epithelial cells. Chinese medical journal, 134(4), 448.

Elkhider, A., Wang, B., Ouyang, X., Al‑Azab, M., Walana, W., Sun, X., ... & Li, X. (2020). Aquaporin 5 promotes tumor migration and angiogenesis in non‑small cell lung cancer cell line H1299. Oncology letters, 19(3), 1665-1672.

Rodrigues, C., Pimpão, C., Mósca, A. F., Coxixo, A. S., Lopes, D., da Silva, I. V., ... & Soveral, G. (2019). Human aquaporin-5 facilitates hydrogen peroxide permeation affecting adaption to oxidative stress and cancer cell migration. Cancers, 11(7), 932.

Rump, K., Unterberg, M., Dahlke, A., Nowak, H., Koos, B., Bergmann, L., ... & Rahmel, T. (2019). DNA methylation of a NF-κB binding site in the aquaporin 5 promoter impacts on mortality in sepsis. Scientific reports, 9(1), 1-11.

Lee, S. Y., Ha, E. J., Cho, H. W., Kim, H. R., Lee, D., & Eom, Y. B. (2019). Potential forensic application of receptor for advanced glycation end products (RAGE) and aquaporin 5 (AQP5) as novel biomarkers for diagnosis of drowning. Journal of forensic and legal medicine, 62, 56-62.

Kumari, S. S., Varadaraj, M., Menon, A. G., & Varadaraj, K. (2018). Aquaporin 5 promotes corneal wound healing. Experimental eye research, 172, 152-158.

Chen, C., Ma, T., Zhang, C., Zhang, H., Bai, L., Kong, L., & Luo, J. (2017). Down‐regulation of aquaporin 5‐mediated epithelial‐mesenchymal transition and anti‐metastatic effect by natural product Cairicoside E in colorectal cancer. Molecular carcinogenesis, 56(12), 2692-2705.

Direito, I., Paulino, J., Vigia, E., Brito, M. A., & Soveral, G. (2017). Differential expression of aquaporin‐3 and aquaporin‐5 in pancreatic ductal adenocarcinoma. Journal of surgical oncology, 115(8), 980-996.

Flodby, P., Li, C., Liu, Y., Wang, H., Rieger, M. E., Minoo, P., ... & Zhou, B. (2017). Cell-specific expression of aquaporin-5 (Aqp5) in alveolar epithelium is directed by GATA6/Sp1 via histone acetylation. Scientific reports, 7(1), 1-12.

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For research use only. Not intended for any clinical use.

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