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Mouse Anti-ARFIP1 Recombinant Antibody (1F10) (CBMAB-A3455-YC)

Provided herein is a Mouse monoclonal antibody against Human ADP Ribosylation Factor Interacting Protein 1. The antibody can be used for immunoassay techniques, such as ELISA, IHC-P, WB.
See all ARFIP1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
1F10
Antibody Isotype
IgG2a, κ
Application
ELISA, IHC-P, WB

Basic Information

Immunogen
ARFIP1 (NP_055262.1, 1 a.a. ~ 101 a.a) partial recombinant protein with GST tag.
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
IHC-P3 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.4
Preservative
None
Concentration
Batch dependent
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
ADP Ribosylation Factor Interacting Protein 1
Introduction
ARFIP1 is a putative target protein of ADP-ribosylation factor.
Entrez Gene ID
27236
UniProt ID
P53367
Alternative Names
ADP Ribosylation Factor Interacting Protein 1; ADP-Ribosylation Factor-Interacting Protein 1; Arfaptin 1; Arfaptin-1; HSU52521;
Function
Plays a role in controlling biogenesis of secretory granules at the trans-Golgi network. Mechanisitically, binds ARF-GTP at the neck of a growing secretory granule precursor and forms a protective scaffold. Once the granule precursor has been completely loaded, active PRKD1 phosphorylates ARFIP1 and releases it from ARFs. In turn, ARFs induce fission. Through this mechanism, ensures proper secretory granule formation at the Golgi of pancreatic beta cells.
Biological Process
Intracellular protein transport Source: MGI
Negative regulation of retrograde transport, endosome to Golgi Source: CACAO
Regulation of Arp2/3 complex-mediated actin nucleation Source: GO_Central
Regulation of protein secretion Source: MGI
Cellular Location
Golgi apparatus; Trans-Golgi network membrane
PTM
Phosphorylated by PRKD1; phosphorylation delocalizes ARFIP1 from the Golgi and disrupts its ability to inhibit the activity of ADP-ribosylation factor, an important component of the vesicle scission machinery.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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