ARFIP1

Putative target protein of ADP-ribosylation factor.

ADP Ribosylation Factor Interacting Protein 1

Plays a role in controlling biogenesis of secretory granules at the trans-Golgi network. Mechanisitically, binds ARF-GTP at the neck of a growing secretory granule precursor and forms a protective scaffold. Once the granule precursor has been completely loaded, active PRKD1 phosphorylates ARFIP1 and releases it from ARFs. In turn, ARFs induce fission. Through this mechanism, ensures proper secretory granule formation at the Golgi of pancreatic beta cells.

Intracellular protein transport Source: MGI
Negative regulation of retrograde transport, endosome to Golgi Source: CACAO
Regulation of Arp2/3 complex-mediated actin nucleation Source: GO_Central
Regulation of protein secretion Source: MGI

Golgi apparatus; Trans-Golgi network membrane

Phosphorylated by PRKD1; phosphorylation delocalizes ARFIP1 from the Golgi and disrupts its ability to inhibit the activity of ADP-ribosylation factor, an important component of the vesicle scission machinery.