Rabbit Anti-ARG2 Recombinant Antibody (
9A4) (V2LY-0725-LY1057)

Name: Rabbit Anti-ARG2 Recombinant Antibody (9A4)
SKU: V2LY-0725-LY1057
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-ARG2 Recombinant Antibody (9A4) (V2LY-0725-LY1057) in IHC

IHC image of V2LY-0725-LY1057 diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Rabbit Anti-ARG2 Recombinant Antibody (9A4) (V2LY-0725-LY1057) in IF

Immunofluorescence staining of PC-3 cell with V2LY-0725-LY1057 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 559-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Rabbit Anti-ARG2 Recombinant Antibody (9A4) (V2LY-0725-LY1057) in FC

Overlay Peak curve showing PC3 cells stained with V2LY-0725-LY1057 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of>10,000 events was performed.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

9A4

Application

ELISA, IHC, IF, FC

Immunogen

A synthesized peptide derived from human ARG2.

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

arginase, type II

Entrez Gene ID

29215

UniProt ID

O08701

Function

May play a role in the regulation of extra-urea cycle arginine metabolism and also in down-regulation of nitric oxide synthesis. Extrahepatic arginase functions to regulate L-arginine bioavailability to nitric oxid synthase (NOS). Arginine metabolism is a critical regulator of innate and adaptive immune responses. Seems to be involved in negative regulation of the survival capacity of activated CD4+ and CD8+ T cells (PubMed:27745970).
May suppress inflammation-related signaling in asthmatic airway epithelium (PubMed:27214549).
May contribute to the immune evasion of H.pylori by restricting M1 macrophage activation and polyamine metabolism (By similarity).
In fetal dendritic cells may play a role in promoting immune suppression and T cell TNF-alpha production during gestation (PubMed:28614294).
Regulates RPS6KB1 signaling, which promotes endothelial cell senescence and inflammation and implicates NOS3/eNOS dysfunction (PubMed:22928666).
Can inhibit endothelial autophagy independently of its enzymatic activity implicating mTORC2 signaling (PubMed:25484082).
Involved in vascular smooth muscle cell senescence and apoptosis independently of its enzymatic activity (PubMed:23832324).
Since NOS is found in the penile corpus cavernosum smooth muscle, the clitoral corpus cavernosum and the vagina, arginase-2 plays a role in both male and female sexual arousal (PubMed:12859189).

Biological Process

Adaptive immune response Source: UniProtKB-KW
Arginine catabolic process to ornithine Source: GO_Central
Innate immune response Source: UniProtKB-KW
Negative regulation of activated CD8-positive, alpha-beta T cell apoptotic process Source: Ensembl
Negative regulation of CD4-positive, alpha-beta T cell proliferation Source: Ensembl
Negative regulation of chemokine (C-C motif) ligand 4 production Source: Ensembl
Negative regulation of chemokine (C-C motif) ligand 5 production Source: Ensembl
Negative regulation of defense response to bacterium Source: Ensembl
Negative regulation of interleukin-13 production Source: Ensembl
Negative regulation of interleukin-17 production Source: Ensembl
Negative regulation of macrophage inflammatory protein 1 alpha production Source: Ensembl
Negative regulation of tumor necrosis factor production Source: UniProtKB
Negative regulation of type 2 immune response Source: Ensembl
Nitric oxide biosynthetic process Source: ProtInc
Positive regulation of cellular senescence Source: UniProtKB
Regulation of interleukin-1 beta production Source: Ensembl
Regulation of reactive oxygen species biosynthetic process Source: Ensembl
Striated muscle contraction Source: Ensembl
Urea cycle Source: Reactome
Ureteric bud development Source: Ensembl

Cellular Location

Mitochondrion

Dowling, J. K., Afzal, R., Gearing, L. J., Cervantes-Silva, M. P., Annett, S., Davis, G. M., ... & McCoy, C. E. (2021). Mitochondrial arginase-2 is essential for IL-10 metabolic reprogramming of inflammatory macrophages. Nature communications, 12(1), 1-14.

Wetzel, M. D., Stanley, K., Wang, W. W., Maity, S., Madesh, M., Reeves, W. B., & Awad, A. S. (2020). Selective inhibition of arginase-2 in endothelial cells but not proximal tubules reduces renal fibrosis. JCI insight, 5(19).

Hara, M., Torisu, K., Tomita, K., Kawai, Y., Tsuruya, K., Nakano, T., & Kitazono, T. (2020). Arginase 2 is a mediator of ischemia–reperfusion injury in the kidney through regulation of nitrosative stress. Kidney International, 98(3), 673-685.

Zhang, Y., Higgins, C. B., Fortune, H. M., Chen, P., Stothard, A. I., Mayer, A. L., ... & DeBosch, B. J. (2019). Hepatic arginase 2 (Arg2) is sufficient to convey the therapeutic metabolic effects of fasting. Nature communications, 10(1), 1-16.

i Líndez, A. A. M., Dunand-Sauthier, I., Conti, M., Gobet, F., Núñez, N., Hannich, J. T., ... & Reith, W. (2019). Mitochondrial arginase-2 is a cell‑autonomous regulator of CD8+ T cell function and antitumor efficacy. JCI insight, 4(24).

Ng, K. P., Manjeri, A., Lee, L. M., Chan, Z. E., Tan, C. Y., Tan, Q. D., ... & Ong, S. T. (2018). The arginase inhibitor Nω− hydroxy− nor− arginine (nor− NOHA) induces apoptosis in leukemic cells specifically under hypoxic conditions but CRISPR/Cas9 excludes arginase 2 (ARG2) as the functional target. Plos one, 13(10), e0205254.

Ochocki, J. D., Khare, S., Hess, M., Ackerman, D., Qiu, B., Daisak, J. I., ... & Simon, M. C. (2018). Arginase 2 suppresses renal carcinoma progression via biosynthetic cofactor pyridoxal phosphate depletion and increased polyamine toxicity. Cell metabolism, 27(6), 1263-1280.

Pandey, D., Nomura, Y., Rossberg, M. C., Hori, D., Bhatta, A., Keceli, G., ... & Romer, L. (2018). Hypoxia triggers SENP1 (Sentrin-Specific Protease 1) modulation of KLF15 (Kruppel-Like Factor 15) and transcriptional regulation of Arg2 (Arginase 2) in pulmonary endothelium. Arteriosclerosis, thrombosis, and vascular biology, 38(4), 913-926.

Zaytouni, T., Tsai, P. Y., Hitchcock, D. S., DuBois, C. D., Freinkman, E., Lin, L., ... & Kalaany, N. Y. (2017). Critical role for arginase 2 in obesity-associated pancreatic cancer. Nature communications, 8(1), 1-12.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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