Mouse Anti-ARHGAP4 Recombinant Antibody (3F7) (CBMAB-A0456-LY)

Name: Mouse Anti-ARHGAP4 Recombinant Antibody (3F7)
SKU: CBMAB-A0456-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

3F7

Application

WB, ELISA

Immunogen

ARHGAP4 (AAH52303, 881 a.a. ~ 986 a.a) partial recombinant protein with GST tag.

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4

Preservative

None

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Rho GTPase activating protein 4

Introduction

This gene encodes a member of the rhoGAP family of proteins which play a role in the regulation of small GTP-binding proteins belonging to the RAS superfamily. The protein encoded by the orthologous gene in rat is localized to the Golgi complex and can redistribute to microtubules. The rat protein stimulates the activity of some Rho GTPases in vitro. Genomic deletions of this gene and a neighboring gene have been found in patients with nephrogenic diabetes insipidus. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Entrez Gene ID

393

UniProt ID

P98171

Alternative Names

C1; KIAA0131; RGC1; RhoGAP4; p115

Function

Inhibitory effect on stress fiber organization. May down-regulate Rho-like GTPase in hematopoietic cells.

Biological Process

Cytoskeleton organization Source: ProtInc
Negative regulation of axon extension Source: Ensembl
Negative regulation of cell migration Source: GO_Central
Negative regulation of fibroblast migration Source: Ensembl
Regulation of small GTPase mediated signal transduction Source: Reactome
Rho protein signal transduction Source: ProtInc

Cellular Location

Cytoplasm. Just below the plasma membrane.

Kang, N., Matsui, T. S., Liu, S., Fujiwara, S., & Deguchi, S. (2020). Comprehensive analysis on the whole Rho‐GAP family reveals that ARHGAP4 suppresses EMT in epithelial cells under negative regulation by Septin9. The FASEB Journal, 34(6), 8326-8340.

Shen, Y., Chen, G., Gao, H., Li, Y., Zhuang, L., Meng, Z., & Liu, L. (2020). miR-939-5p Contributes to the migration and invasion of pancreatic cancer by targeting ARHGAP4. OncoTargets and therapy, 13, 389.

Shen, Y., Chen, G., Zhuang, L., Xu, L., Lin, J., & Liu, L. (2019). ARHGAP4 mediates the Warburg effect in pancreatic cancer through the mTOR and HIF-1α signaling pathways. OncoTargets and therapy, 12, 5003.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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