Mouse Anti-ARHGEF9 Recombinant Antibody (3C11) (CBMAB-A0473-LY)

The product is antibody recognizes ARHGEF9. The antibody 3C11 immunoassay techniques such as: WB, ELISA.
See all ARHGEF9 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

3C11

Antibody Isotype

IgG2a, κ

Application

WB, ELISA

Basic Information

Immunogen

ARHGEF9 (NP_056000.1, 419 a.a. ~ 516 a.a) partial recombinant protein with GST tag.

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4

Preservative

None

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

Cdc42 Guanine Nucleotide Exchange Factor 9

Introduction

ARHGEF9 belongs to a family of Rho-like GTPases that act as molecular switches by cycling from the active GTP-bound state to the inactive GDP-bound state. These proteins are key regulators of the actin cytoskeleton and are involved in cell signaling.[supplied by OMIM

Entrez Gene ID

23229

UniProt ID

O43307

Alternative Names

COLLYBISTIN; HPEM-2; KIAA0424; PEM-2; PEM2

Function

Acts as guanine nucleotide exchange factor (GEF) for CDC42. Promotes formation of GPHN clusters (By similarity).

Biological Process

G protein-coupled receptor signaling pathway Source: Reactome
Positive regulation of apoptotic process Source: Reactome
Regulation of small GTPase mediated signal transduction Source: Reactome

Cellular Location

Cytoplasm; Postsynaptic density

Involvement in disease

Developmental and epileptic encephalopathy 8 (DEE8): A disorder characterized by hyperekplexia and early infantile epileptic encephalopathy. Neurologic features include exaggerated startle response, seizures, impaired psychomotor development, and mental retardation. Seizures can be provoked by tactile stimulation or extreme emotion.

References

Ghesh, L., Besnard, T., Nizon, M., Trochu, E., Landeau‐Trottier, G., Breheret, F., ... & Cogné, B. (2021). Loss‐of‐function variants in ARHGEF9 are associated with an X‐linked intellectual disability dominant disorder. Human Mutation, 42(5), 498-505.

Yao, R., Zhang, Y., Liu, J., Wang, J., Xu, Y., Li, N., ... & Yu, T. (2020). Clinical and molecular characterization of three novel ARHGEF9 mutations in patients with developmental delay and epilepsy. Journal of Molecular Neuroscience, 1-8.

Wang, J. Y., Zhou, P., Wang, J., Tang, B., Su, T., Liu, X. R., ... & Liao, W. P. (2018). ARHGEF9 mutations in epileptic encephalopathy/intellectual disability: toward understanding the mechanism underlying phenotypic variation. Neurogenetics, 19(1), 9-16.

Ibaraki, K., Mizuno, M., Aoki, H., Niwa, A., Iwamoto, I., Hara, A., ... & Nagata, K. I. (2018). Biochemical and morphological characterization of a guanine nucleotide exchange factor ARHGEF9 in mouse tissues. Acta histochemica et cytochemica, 51(3), 119-128.

Striano, P., & Zara, F. (2017). ARHGEF9 mutations cause a specific recognizable X-linked intellectual disability syndrome.

Q & As

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Review & reward

Have you used Mouse Anti-ARHGEF9 Recombinant Antibody (3C11)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Related Products

Mouse Anti-ARHGEF9 Recombinant Antibody (CBYY-C3173)

Mouse Anti-ARHGEF9 Recombinant Antibody (161B11)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Datasheet
SDS
Request for COA

Request bulk or custom quote

Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email: