Mouse Anti-ARHGEF9 Recombinant Antibody (CBYY-C3173) (CBMAB-C4616-YY)

This product is mouse antibody that recognizes ARHGEF9. The antibody CBYY-C3173 can be used for immunoassay techniques such as: WB, IHC
See all ARHGEF9 antibodies

Published Data

Mouse Anti-ARHGEF9 Recombinant Antibody (CBYY-C3173) CBMAB-C4616-YY in WB

Western blot showing the expression of Gephyrin, the alpha1 subunit of the GABAA receptor, PSD-95, the GluA1 subunit of the AMPA receptor, the GluN2B subunit of the NMDA receptor, or the Gephyrin interacting proteins Collybistin II and GABARAP in neurons not transduced (Control) or transduced with GFE3, RandE3 or GPHN. ...View More

Gross, G. G., Straub, C., Perez-Sanchez, J., Dempsey, W. P., Junge, J. A., Roberts, R. W., ... & Arnold, D. B. (2016). An E3-ligase-based method for ablating inhibitory synapses. nAture methods, 13(8), 673-678.

Datasheet

Summary

Host Animal

Mouse

Specificity

Human, Mouse, Rat

Clone

CBYY-C3173

Antibody Isotype

IgG1, κ

Application

WB, IP

Basic Information

Immunogen

Amino acids 18-131 of ARHGEF9 of rat origin.

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

Cdc42 Guanine Nucleotide Exchange Factor 9

Entrez Gene ID

23229

UniProt ID

O43307

Function

Acts as guanine nucleotide exchange factor (GEF) for CDC42. Promotes formation of GPHN clusters (By similarity).

Biological Process

G protein-coupled receptor signaling pathway Source: Reactome
Positive regulation of apoptotic process Source: Reactome
Regulation of small GTPase mediated signal transduction Source: Reactome

Cellular Location

Cytoplasm; Postsynaptic density

Involvement in disease

Developmental and epileptic encephalopathy 8 (DEE8): A disorder characterized by hyperekplexia and early infantile epileptic encephalopathy. Neurologic features include exaggerated startle response, seizures, impaired psychomotor development, and mental retardation. Seizures can be provoked by tactile stimulation or extreme emotion.

References

Ghesh, L., Besnard, T., Nizon, M., Trochu, E., Landeau‐Trottier, G., Breheret, F., ... & Cogné, B. (2021). Loss‐of‐function variants in ARHGEF9 are associated with an X‐linked intellectual disability dominant disorder. Human Mutation, 42(5), 498-505.

Yao, R., Zhang, Y., Liu, J., Wang, J., Xu, Y., Li, N., ... & Yu, T. (2020). Clinical and molecular characterization of three novel ARHGEF9 mutations in patients with developmental delay and epilepsy. Journal of Molecular Neuroscience, 1-8.

Wang, J. Y., Zhou, P., Wang, J., Tang, B., Su, T., Liu, X. R., ... & Liao, W. P. (2018). ARHGEF9 mutations in epileptic encephalopathy/intellectual disability: toward understanding the mechanism underlying phenotypic variation. Neurogenetics, 19(1), 9-16.

Ibaraki, K., Mizuno, M., Aoki, H., Niwa, A., Iwamoto, I., Hara, A., ... & Nagata, K. I. (2018). Biochemical and morphological characterization of a guanine nucleotide exchange factor ARHGEF9 in mouse tissues. Acta histochemica et cytochemica, 51(3), 119-128.

Striano, P., & Zara, F. (2017). ARHGEF9 mutations cause a specific recognizable X-linked intellectual disability syndrome.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Related Products

Mouse Anti-ARHGEF9 Recombinant Antibody (3C11)

Mouse Anti-ARHGEF9 Recombinant Antibody (161B11)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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