Rabbit Anti-ARID4A Recombinant Antibody (CBCNR-283) (CBMAB-R0423-CN)

Name: Rabbit Anti-ARID4A Recombinant Antibody (CBCNR-283)
SKU: CBMAB-R0423-CN
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

CBCNR-283

Application

WB, IP

Immunogen

Synthetic peptide corresponding to residues surrounding Gly813 of human ARID4A/RbBP1 protein.

Specificity

Human, Monkey

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:1,000
IP	1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

10mM HEPES, pH 7.5, 0.15M NaCl, 0.1mg/mL BSA, 50% Glycerol

Preservative

0.02% sodium azide

Concentration

Batch dependent

More Infomation

Target

Full Name

AT rich interactive domain 4A (RBP1-like)

Introduction

The protein encoded by this gene is a ubiquitously expressed nuclear protein. It binds directly, with several other proteins, to retinoblastoma protein (pRB) which regulates cell proliferation. pRB represses transcription by recruiting the encoded protein. This protein, in turn, serves as a bridging molecule to recruit HDACs and, in addition, provides a second HDAC-independent repression function. The encoded protein possesses transcriptional repression activity. Multiple alternatively spliced transcripts have been observed for this gene, although not all transcript variants have been fully described. [provided by RefSeq, Jul 2008]

Entrez Gene ID

5926

UniProt ID

P29374

Alternative Names

AT-Rich Interaction Domain 4A; Retinoblastoma-Binding Protein 1; AT Rich Interactive Domain 4A (RBP1-Like); ARID Domain-Containing Protein 4A; RBBP-1; RBBP1; RBP1; AT-Rich Interactive Domain-Containing Protein 4A; RBP-1;

Function

DNA-binding protein which modulates activity of several transcription factors including RB1 (retinoblastoma-associated protein) and AR (androgen receptor) (By similarity).
May function as part of an mSin3A repressor complex (PubMed:14581478).
Has no intrinsic transcriptional activity (By similarity).
Plays a role in the regulation of epigenetic modifications at the PWS/AS imprinting center near the SNRPN promoter, where it might function as part of a complex with RB1 and ARID4B (By similarity).
Involved in spermatogenesis, together with ARID4B, where it acts as a transcriptional coactivator for AR and enhances expression of genes required for sperm maturation. Regulates expression of the tight junction protein CLDN3 in the testis, which is important for integrity of the blood-testis barrier (By similarity).
Plays a role in myeloid homeostasis where it regulates the histone methylation state of bone marrow cells and expression of various genes involved in hematopoiesis. May function as a leukemia suppressor (By similarity).

Biological Process

Erythrocyte development Source: Ensembl
Establishment of Sertoli cell barrier Source: Ensembl
Histone H3-K4 trimethylation Source: Ensembl
Histone H3-K9 trimethylation Source: Ensembl
Histone H4-K20 trimethylation Source: Ensembl
Negative regulation of transcription, DNA-templated Source: GDB
Positive regulation of transcription by RNA polymerase II Source: Ensembl
Regulation of gene expression by genetic imprinting Source: Ensembl
Regulation of transcription by RNA polymerase II Source: GO_Central
Spermatogenesis Source: UniProtKB-KW

Cellular Location

Nucleus

Liang, Y. K., Han, Z. D., Lu, J. M., Liu, Z. Z., Zhuo, Y. J., Zhu, X. J., ... & Zhong, W. D. (2018). Downregulation of ARID4A and ARID4B promote tumor progression and directly regulated by microRNA‐30d in patient with prostate cancer. Journal of cellular biochemistry, 119(9), 7245-7255.

Yang, C., Wang, J., Liu, J., Sun, Y., Guo, Y., Jiang, Q., ... & Wang, C. (2018). Functional haplotypes of ARID4A affect promoter activity and semen quality of bulls. Animal reproduction science, 197, 257-267.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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