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Mouse Anti-ARIH2 Recombinant Antibody (1C3) (CBMAB-A3565-YC)

Provided herein is a Mouse monoclonal antibody against Human Ariadne RBR E3 Ubiquitin Protein Ligase 2. The antibody can be used for immunoassay techniques, such as ELISA, WB.
See all ARIH2 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
1C3
Antibody Isotype
IgM, κ
Application
ELISA, WB

Basic Information

Immunogen
Partial recombinant corresponding to aa331-420 from ARIH2 (NP_006312) with GST tag.
Specificity
Human
Antibody Isotype
IgM, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.4
Preservative
None
Concentration
Batch dependent
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Ariadne RBR E3 Ubiquitin Protein Ligase 2
Introduction
ARIH2 is an E3 ubiquitin-protein ligase that polyubiquitinates some proteins, tagging them for degradation. The encoded protein upregulates p53 in some cancer cells and may inhibit myelopoiesis. Several transcript variants encoding different isoforms have
Entrez Gene ID
10425
UniProt ID
O95376
Alternative Names
Ariadne RBR E3 Ubiquitin Protein Ligase 2; All-Trans Retinoic Acid Inducible RING Finger; RING-Type E3 Ubiquitin Transferase ARIH2; Protein Ariadne-2 Homolog; TRIAD1; ARI2; E3 Ubiquitin-Protein Ligase ARIH2;
Function
E3 ubiquitin-protein ligase, which catalyzes ubiquitination of target proteins together with ubiquitin-conjugating enzyme E2 UBE2L3 (PubMed:16118314, PubMed:17646546, PubMed:19340006, PubMed:24076655).
Acts as an atypical E3 ubiquitin-protein ligase by working together with cullin-5-RING ubiquitin ligase complex (ECS complex, also named CRL5 complex) and initiating ubiquitination of ECS substrates: associates with ECS complex and specifically mediates addition of the first ubiquitin on ECS targets (By similarity).
The initial ubiquitin is then elongated (By similarity).
E3 ubiquitin-protein ligase activity is activated upon binding to neddylated form of the ECS complex (PubMed:24076655).
Mediates 'Lys-6', 'Lys-48'- and 'Lys-63'-linked polyubiquitination (PubMed:16118314, PubMed:17646546, PubMed:19340006).
May play a role in myelopoiesis (PubMed:19340006).
Biological Process
Developmental cell growth Source: UniProtKB
Hematopoietic stem cell proliferation Source: UniProtKB
Multicellular organism development Source: ProtInc
Positive regulation of proteasomal ubiquitin-dependent protein catabolic process Source: GO_Central
Positive regulation of protein targeting to mitochondrion Source: ParkinsonsUK-UCL
Protein K48-linked ubiquitination Source: UniProtKB
Protein K63-linked ubiquitination Source: UniProtKB
Protein polyubiquitination Source: UniProtKB
Protein ubiquitination Source: UniProtKB
Ubiquitin-dependent protein catabolic process Source: UniProtKB
Cellular Location
Cytoplasm; Nucleus
PTM
Ubiquitinated. Ubiquitination promotes proteasomal degradation.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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