Mouse Anti-ARIH2 Recombinant Antibody (CBYC-A770) (CBMAB-A3568-YC)

Name: Mouse Anti-ARIH2 Recombinant Antibody (CBYC-A770)
SKU: CBMAB-A3568-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBYC-A770

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 314-481 mapping near the C-terminus of TRIAD1 of human origin.

Specificity

Human, Mouse, Rat, Cattle, Chicken, Horse, Pig

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Ariadne RBR E3 Ubiquitin Protein Ligase 2

Introduction

ARIH2 is an E3 ubiquitin-protein ligase that polyubiquitinates some proteins, tagging them for degradation. The encoded protein upregulates p53 in some cancer cells and may inhibit myelopoiesis. Several transcript variants encoding different isoforms have

Entrez Gene ID

10425

UniProt ID

O95376

Alternative Names

Ariadne RBR E3 Ubiquitin Protein Ligase 2; All-Trans Retinoic Acid Inducible RING Finger; RING-Type E3 Ubiquitin Transferase ARIH2; Protein Ariadne-2 Homolog; TRIAD1; ARI2; E3 Ubiquitin-Protein Ligase ARIH2;

Function

E3 ubiquitin-protein ligase, which catalyzes ubiquitination of target proteins together with ubiquitin-conjugating enzyme E2 UBE2L3 (PubMed:16118314, PubMed:17646546, PubMed:19340006, PubMed:24076655).
Acts as an atypical E3 ubiquitin-protein ligase by working together with cullin-5-RING ubiquitin ligase complex (ECS complex, also named CRL5 complex) and initiating ubiquitination of ECS substrates: associates with ECS complex and specifically mediates addition of the first ubiquitin on ECS targets (By similarity).
The initial ubiquitin is then elongated (By similarity).
E3 ubiquitin-protein ligase activity is activated upon binding to neddylated form of the ECS complex (PubMed:24076655).
Mediates 'Lys-6', 'Lys-48'- and 'Lys-63'-linked polyubiquitination (PubMed:16118314, PubMed:17646546, PubMed:19340006).
May play a role in myelopoiesis (PubMed:19340006).

Biological Process

Developmental cell growth Source: UniProtKB
Hematopoietic stem cell proliferation Source: UniProtKB
Multicellular organism development Source: ProtInc
Positive regulation of proteasomal ubiquitin-dependent protein catabolic process Source: GO_Central
Positive regulation of protein targeting to mitochondrion Source: ParkinsonsUK-UCL
Protein K48-linked ubiquitination Source: UniProtKB
Protein K63-linked ubiquitination Source: UniProtKB
Protein polyubiquitination Source: UniProtKB
Protein ubiquitination Source: UniProtKB
Ubiquitin-dependent protein catabolic process Source: UniProtKB

Cellular Location

Cytoplasm; Nucleus

PTM

Ubiquitinated. Ubiquitination promotes proteasomal degradation.

Kelsall, I. R., Kristariyanto, Y. A., Knebel, A., Wood, N. T., Kulathu, Y., & Alpi, A. F. (2019). Coupled monoubiquitylation of the co-E3 ligase DCNL1 by Ariadne-RBR E3 ubiquitin ligases promotes cullin-RING ligase complex remodeling. Journal of Biological Chemistry, 294(8), 2651-5314.

Wu, G., Xu, S., Zhang, W., Liu, Y., Wang, Q., & Man, C. (2019). Arih2 gene influences immune response and tissue development in chicken. Bioscience reports, 39(10), BSR20190933.

Kawashima, A., Karasawa, T., Tago, K., Kimura, H., Kamata, R., Usui-Kawanishi, F., ... & Takahashi, M. (2017). ARIH2 ubiquitinates NLRP3 and negatively regulates NLRP3 inflammasome activation in macrophages. The Journal of Immunology, 199(10), 3614-3622.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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