Mouse Anti-ARL1 Recombinant Antibody (CBYC-A771) (CBMAB-A3569-YC)

Name: Mouse Anti-ARL1 Recombinant Antibody (CBYC-A771)
SKU: CBMAB-A3569-YC
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-ARL1 Recombinant Antibody (CBYC-A771) CBMAB-A3569-YC in IF

Representative examples of neurons immunostained for a cis-/medial Golgi marker (GM130 or giantin), TGN38 and a protein of interest (ARL1, SMIM7, HID1 and TMEM87A). Images showing immunostaining of cis-/medial Golgi marker and TGN (a), TGN and protein of interest (b), cis-/medial Golgi and protein of interest (c). ...View More

van Bommel, D. M., Toonen, R. F., & Verhage, M. (2023). Mapping localization of 21 endogenous proteins in the Golgi apparatus of rodent neurons. Scientific Reports, 13(1), 2871.

Mouse Anti-ARL1 Recombinant Antibody (CBYC-A771) CBMAB-A3569-YC in ICC

Representative examples of neurons immunostained for ARL1. ...View More

van Bommel, D. M., Toonen, R. F., & Verhage, M. (2022). Vti1a/b support distinct aspects of TGN and cis-/medial Golgi organization. Scientific Reports, 12(1), 20870.

Mouse Anti-ARL1 Recombinant Antibody (CBYC-A771) CBMAB-A3569-YC in IP

Co-immunoprecipitation of ARG2 with ARL1. Cells were treated with H2O2 (200 μM) for 24 h, and the interaction between ARG2 and ARL1 was examined by Western blotting. ...View More

Kim, H. J., Kim, B., Byun, H. J., Yu, L., Nguyen, T. M., Nguyen, T. H., ... & Lee, C. H. (2021). Resolvin D1 suppresses H2O2-induced senescence in fibroblasts by inducing autophagy through the miR-1299/ARG2/ARL1 axis. Antioxidants, 10(12), 1924.

Mouse Anti-ARL1 Recombinant Antibody (CBYC-A771) CBMAB-A3569-YC in IF

Confocal microscopic analysis of ARG2 and ARL1 expression. Cells were treated with H2O2 for 24 h and examined by confocal microscopy. DAPI staining was used to identify the nucleus. Bar, 10 mm. ...View More

Datasheet

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBYC-A771

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 128-181 mapping at the C-terminus of ARL1 of human origin.

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

ADP Ribosylation Factor Like GTPase 1

Introduction

ARL1 belongs to the ARL (ADP-ribosylation factor-like) family of proteins, which are structurally related to ADP-ribosylation factors (ARFs). ARFs, described as activators of cholera toxin (CT) ADP-ribosyltransferase activity, regulate intracellular vesic

Entrez Gene ID

400

UniProt ID

P40616

Alternative Names

ADP Ribosylation Factor Like GTPase 1; ADP-Ribosylation Factor-Like 1; ADP-Ribosylation Factor-Like Protein 1; ARFL1;

Function

GTP-binding protein that recruits several effectors, such as golgins, arfaptins and Arf-GEFs to the trans-Golgi network, and modulates their functions at the Golgi complex (PubMed:9624189, PubMed:21239483, PubMed:27436755, PubMed:22679020, PubMed:27373159).
Plays thereby a role in a wide range of fundamental cellular processes, including cell polarity, innate immunity, or protein secretion mediated by arfaptins, which were shown to play a role in maintaining insulin secretion from pancreatic beta cells (PubMed:22981988).

Biological Process

Activation of phospholipase D activity Source: UniProtKB
Golgi organization Source: UniProtKB
Intracellular protein transport Source: GO_Central
Protein localization to Golgi apparatus Source: UniProtKB
Retrograde transport, endosome to Golgi Source: UniProtKB
Toxin metabolic process Source: UniProtKB
Vesicle-mediated transport Source: GO_Central

Cellular Location

Golgi apparatus membrane; Trans-Golgi network membrane; Membrane

Feng, H. P., Cheng, H. Y., Hsiao, T. F., Lin, T. W., Hsu, J. W., Huang, L. H., & Yu, C. J. (2021). ArfGAP1 acts as a GTPase‐activating protein for human ADP‐ribosylation factor‐like 1 protein. The FASEB Journal, 35(4), e21337.

Yu, C. J., & Lee, F. J. S. (2017). Multiple activities of Arl1 GTPase in the trans-Golgi network. Journal of cell science, 130(10), 1691-1699.

Gadahi, J. A., Ehsan, M., Wang, S., Zhang, Z., Yan, R., Song, X., ... & Li, X. (2017). Recombinant protein of Haemonchus contortus small GTPase ADP-ribosylation factor 1 (HcARF1) modulate the cell mediated immune response in vitro. Oncotarget, 8(68), 112211.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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