Sign in or Register   Sign in or Register
  |  

Mouse Anti-ARL1 Recombinant Antibody (CBYC-A771) (CBMAB-A3569-YC)

Provided herein is a Rabbit monoclonal antibody against Human ADP Ribosylation Factor Like GTPase 1. The antibody can be used for immunoassay techniques, such as IF, ICC, IHC, FC.
See all ARL1 antibodies
Published Data
Mouse Anti-ARL1 Recombinant Antibody (CBYC-A771) CBMAB-A3569-YC in IF
Representative examples of neurons immunostained for a cis-/medial Golgi marker (GM130 or giantin), TGN38 and a protein of interest (ARL1, SMIM7, HID1 and TMEM87A). Images showing immunostaining of cis-/medial Golgi marker and TGN (a), TGN and protein of interest (b), cis-/medial Golgi and protein of interest (c). ...View More
van Bommel, D. M., Toonen, R. F., & Verhage, M. (2023). Mapping localization of 21 endogenous proteins in the Golgi apparatus of rodent neurons. Scientific Reports, 13(1), 2871.
Mouse Anti-ARL1 Recombinant Antibody (CBYC-A771) CBMAB-A3569-YC in ICC
Representative examples of neurons immunostained for ARL1. ...View More
van Bommel, D. M., Toonen, R. F., & Verhage, M. (2022). Vti1a/b support distinct aspects of TGN and cis-/medial Golgi organization. Scientific Reports, 12(1), 20870.
Mouse Anti-ARL1 Recombinant Antibody (CBYC-A771) CBMAB-A3569-YC in IP
Co-immunoprecipitation of ARG2 with ARL1. Cells were treated with H2O2 (200 μM) for 24 h, and the interaction between ARG2 and ARL1 was examined by Western blotting. ...View More
Kim, H. J., Kim, B., Byun, H. J., Yu, L., Nguyen, T. M., Nguyen, T. H., ... & Lee, C. H. (2021). Resolvin D1 suppresses H2O2-induced senescence in fibroblasts by inducing autophagy through the miR-1299/ARG2/ARL1 axis. Antioxidants, 10(12), 1924.
Mouse Anti-ARL1 Recombinant Antibody (CBYC-A771) CBMAB-A3569-YC in IF
Confocal microscopic analysis of ARG2 and ARL1 expression. Cells were treated with H2O2 for 24 h and examined by confocal microscopy. DAPI staining was used to identify the nucleus. Bar, 10 mm. ...View More
Kim, H. J., Kim, B., Byun, H. J., Yu, L., Nguyen, T. M., Nguyen, T. H., ... & Lee, C. H. (2021). Resolvin D1 suppresses H2O2-induced senescence in fibroblasts by inducing autophagy through the miR-1299/ARG2/ARL1 axis. Antioxidants, 10(12), 1924.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBYC-A771
Antibody Isotype
IgG1, κ
Application
WB, IP, IF, ELISA

Basic Information

Immunogen
Amino acids 128-181 mapping at the C-terminus of ARL1 of human origin.
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
ADP Ribosylation Factor Like GTPase 1
Introduction
ARL1 belongs to the ARL (ADP-ribosylation factor-like) family of proteins, which are structurally related to ADP-ribosylation factors (ARFs). ARFs, described as activators of cholera toxin (CT) ADP-ribosyltransferase activity, regulate intracellular vesic
Entrez Gene ID
400
UniProt ID
P40616
Alternative Names
ADP Ribosylation Factor Like GTPase 1; ADP-Ribosylation Factor-Like 1; ADP-Ribosylation Factor-Like Protein 1; ARFL1;
Function
GTP-binding protein that recruits several effectors, such as golgins, arfaptins and Arf-GEFs to the trans-Golgi network, and modulates their functions at the Golgi complex (PubMed:9624189, PubMed:21239483, PubMed:27436755, PubMed:22679020, PubMed:27373159).
Plays thereby a role in a wide range of fundamental cellular processes, including cell polarity, innate immunity, or protein secretion mediated by arfaptins, which were shown to play a role in maintaining insulin secretion from pancreatic beta cells (PubMed:22981988).
Biological Process
Activation of phospholipase D activity Source: UniProtKB
Golgi organization Source: UniProtKB
Intracellular protein transport Source: GO_Central
Protein localization to Golgi apparatus Source: UniProtKB
Retrograde transport, endosome to Golgi Source: UniProtKB
Toxin metabolic process Source: UniProtKB
Vesicle-mediated transport Source: GO_Central
Cellular Location
Golgi apparatus membrane; Trans-Golgi network membrane; Membrane
Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-ARL1 Recombinant Antibody (CBYC-A771)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Go to
Compare