Rabbit Anti-ARNT Recombinant Antibody (
2F11) (V2LY-0725-LY604)

Name: Rabbit Anti-ARNT Recombinant Antibody (2F11)
SKU: V2LY-0725-LY604
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-ARNT Recombinant Antibody (2F11) (V2LY-0725-LY604) in WB

Western Blot
Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, K562 whole cell lysate
All lanes: ARNT antibody at 1.83μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 87, 85, 86 KDa
Observed band size: 87 KDa

Rabbit Anti-ARNT Recombinant Antibody (2F11) (V2LY-0725-LY604) in IHC

IHC image of V2LY-0725-LY604 diluted at 1:183 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Rabbit Anti-ARNT Recombinant Antibody (2F11) (V2LY-0725-LY604) in IP

Immunoprecipitating HIF-1 beta in Hela whole cell lysate
Lane 1: Rabbit control IgG instead of V2LY-0725-LY604 in Hela whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: V2LY-0725-LY604 (3μg) + Hela whole cell lysate (500μg)
Lane 3: Hela whole cell lysate (20μg)

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

2F11

Application

ELISA, WB, IHC, IP

Immunogen

A synthesized peptide derived from human ARNT.

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:5,000
IHC	1:50-1:200
IP	1:200-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Aryl Hydrocarbon Receptor Nuclear Translocator

Entrez Gene ID

405

UniProt ID

P27540

Alternative Names

Aryl Hydrocarbon Receptor Nuclear Translocator; Class E Basic Helix-Loop-Helix Protein 2; Dioxin Receptor, Nuclear Translocator; HIF-1-Beta; HIF1-Beta; BHLHe2; Hypoxia-Inducible Factor 1, Beta Subunit;

Function

Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The complex then initiates transcription of genes involved in the activation of PAH procarcinogens. The heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters and functions as a transcriptional regulator of the adaptive response to hypoxia (By similarity).
The heterodimer ARNT:AHR binds to core DNA sequence 5'-TGCGTG-3' within the dioxin response element (DRE) of target gene promoters and activates their transcription (PubMed:28396409).

Biological Process

Cell differentiation Source: Ensembl
Embryonic placenta development Source: Ensembl
Positive regulation of endothelial cell proliferation Source: BHF-UCL
Positive regulation of erythrocyte differentiation Source: BHF-UCL
Positive regulation of glycolytic process Source: BHF-UCL
Positive regulation of hormone biosynthetic process Source: BHF-UCL
Positive regulation of protein sumoylation Source: Ensembl
Positive regulation of transcription by RNA polymerase II Source: BHF-UCL
Positive regulation of vascular endothelial growth factor production Source: BHF-UCL
Positive regulation of vascular endothelial growth factor receptor signaling pathway Source: BHF-UCL
Regulation of transcription by RNA polymerase II Source: GO_Central
Regulation of transcription from RNA polymerase II promoter in response to hypoxia Source: Reactome
Regulation of transcription from RNA polymerase II promoter in response to oxidative stress Source: BHF-UCL
Response to hypoxia Source: BHF-UCL
Xenobiotic metabolic process Source: Reactome

Cellular Location

Nucleus

Nguyen, T., Zheng, M., Knapp, M., Sladojevic, N., Zhang, Q., Ai, L., ... & Wu, R. (2021). Endothelial Aryl Hydrocarbon Receptor Nuclear Translocator Mediates the Angiogenic Response to Peripheral Ischemia in Mice With Type 2 Diabetes Mellitus. Frontiers in Cell and Developmental Biology, 9, 1478.

Kim, S. A., Jo, S. H., Cho, J. H., Yu, M. Y., Shin, H. C., Kim, J. A., ... & Kim, J. H. (2020). Aryl Sulfonamides Induce Degradation of Aryl Hydrocarbon Receptor Nuclear Translocator through CRL4DCAF15 E3 Ligase. Molecules and cells, 43(11), 935.

Gunin, A. G., Golubtzova, N. N., & Kornilova, N. K. (2020). Aryl-Hydrocarbon Receptor Nuclear Translocator (ARNT) in Human Skin with Aging. Advances in Gerontology, 10(4), 388-392.

Scott, C., Stokes, R., Cha, K. M., Clouston, A., Eslam, M., Metwally, M., ... & Gunton, J. E. (2019). Myeloid cell deletion of Aryl hydrocarbon Receptor Nuclear Translocator (ARNT) induces non-alcoholic steatohepatitis. Plos one, 14(12), e0225332.

Ji, S., Xin, H., & Su, E. J. (2019). Overexpression of the aryl hydrocarbon receptor nuclear translocator partially rescues fetoplacental angiogenesis in severe fetal growth restriction. Clinical Science, 133(12), 1353-1365.

Peng, T., Chen, X., Pan, Y., Zheng, Z., Wei, X., Xi, J., ... & Shang, Q. (2017). Transcription factor aryl hydrocarbon receptor/aryl hydrocarbon receptor nuclear translocator is involved in regulation of the xenobiotic tolerance‐related cytochrome P450 CYP6DA2 in Aphis gossypii Glover. Insect molecular biology, 26(5), 485-495.

Scott, C. H., Cha, K. M., Ngai, J., Jiang, C., Cheng, K., Stokes, R. A., ... & Gunton, J. E. (2017). Hepatic Aryl hydrocarbon Receptor Nuclear Translocator (ARNT) regulates metabolism in mice. Plos one, 12(11), e0186543.

Hunter, S. R., Vonk, A., Grey, A. K. M., & Riddick, D. S. (2017). Role of glucocorticoid receptor and pregnane X receptor in dexamethasone induction of rat hepatic aryl hydrocarbon receptor nuclear translocator and NADPH-cytochrome P450 oxidoreductase. Drug Metabolism and Disposition, 45(2), 118-129.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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