Mouse Anti-ARRB2 Recombinant Antibody (B4) (CBMAB-A3674-YC)

Name: Mouse Anti-ARRB2 Recombinant Antibody (B4)
SKU: CBMAB-A3674-YC
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-ARRB2 Recombinant Antibody (B4) CBMAB-A3674-YC in IP

IP assays for studying GPR120-β-arrestin 2 interactions in WT and AMPKα2−/− VSMCs. All data shown represent one of two separate experiments. Quantification of western blots was performed using ImagePro plus software, distinctly from loading controls. Data are presented as the mean ± s.e.m. p value by two-sided Student's ttests. ...View More

Yan, C. H., Liu, H. W., Tian, X. X., Li, J., Ding, Y., Li, Y., ... & Han, Y. L. (2022). AMPKα2 controls the anti-atherosclerotic effects of fish oils by modulating the SUMOylation of GPR120. Nature Communications, 13(1), 7721.

Mouse Anti-ARRB2 Recombinant Antibody (B4) CBMAB-A3674-YC in WB

Western blot analysis of ARRB2 protein levels in the retina. ...View More

Harun-Or-Rashid, M., & Inman, D. M. (2018). Reduced AMPK activation and increased HCAR activation drive anti-inflammatory response and neuroprotection in glaucoma. Journal of neuroinflammation, 15(1), 1-15.

Mouse Anti-ARRB2 Recombinant Antibody (B4) CBMAB-A3674-YC in Immunoblot

Immunoblotting (IB) analysis of established upstream regulators of canonical Wnt signaling, including APC (adenomatosis polyposis coli protein), ATDC (ataxia-telangiectasia group D-associated protein), Axin, β-arrestin, β-TrCP (β-transducin repeat), CK-1α (casein kinase 1α), Dvl-1, Dvl-2, Dvl-3, GSK-3β (glycogen synthase kinase-3β), PAR-1 (protease activated receptor 1), PP2A (protein phosphatase 2A) and tankyrase, in control- or ASPMKD PC-3 cells. Right, the fold of reduction in the protein abundance level of the indicated Wnt regulator in control- vs. ASPM-KD PC-3 cells. ...View More

Pai, V. C., Hsu, C. C., Chan, T. S., Liao, W. Y., Chuu, C. P., Chen, W. Y., ... & Tsai, K. K. (2019). ASPM promotes prostate cancer stemness and progression by augmenting Wnt− Dvl-3− β-catenin signaling. Oncogene, 38(8), 1340-1353.

Datasheet

SDS

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Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

Application

ELISA, ICC, IF, WB, IHC-P

Immunogen

Amino acids 385-408 at the C-terminus of β-Arrestin-2 of human origin.

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
IHC-P	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

ARRB2

Introduction

Members of arrestin/beta-arrestin protein family are thought to participate in agonist-mediated desensitization of G-protein-coupled receptors and cause specific dampening of cellular responses to stimuli such as hormones, neurotransmitters, or sensory si

Entrez Gene ID

394099

UniProt ID

Q7T2D2

Alternative Names

Arrestin Beta 2; Non-Visual Arrestin-3; Arrestin 3; ARR2; ARB2; Arrestin, Beta 2; Beta-Arrestin-2; Arrestin Beta-2; BARR2;

Cellular Location

Cytoplasm; Nucleus; Cell membrane; Clathrin-coated pit; Cytoplasmic vesicle. Translocates to the plasma membrane and colocalizes with antagonist-stimulated GPCRs.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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